PLCD4 (NM_032726) Human Tagged ORF Clone
CAT#: RG202935
- TrueORF®
PLCD4 (tGFP-tagged) - Human phospholipase C, delta 4 (PLCD4)
ORF Plasmid: DDK
"NM_032726" in other vectors (4)
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CNY 7,184.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG202935 representing NM_032726.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGTCCCTGCTGCAAGACCAGCTGACCACTGATCAGGACTTGCTGCTGATGCAGGAAGGCATGTCG ATGCGCAAGGTGAGGTCCAAAAGCTGGAAGAAGCTAAGATACTTCAGACTTCAGAATGACGGCATGACA GTCTGGCATGCACGGCAGGCCAGGGGCAGTGCCAAGCCCAGCTTCTCAATCTCTGATGTGGAGACAATA CGTAATGGCCATGATTCCGAGTTGCTGCGTAGCCTGGCAGAGGAGCTCCCCCTGGAGCAGGGCTTCACC ATTGTCTTCCATGGCCGCCGCTCCAACCTGGACCTGATGGCCAACAGTGTTGAGGAGGCCCAGATATGG ATGCGAGGGCTCCAGCTGTTGGTGGATCTTGTCACCAGCATGGACCATCAGGAGCGCCTGGACCAATGG CTGAGCGATTGGTTTCAACGTGGAGACAAAAATCAGGATGGTAAGATGAGTTTCCAAGAAGTTCAGCGG TTATTGCACCTAATGAATGTGGAAATGGACCAAGAATATGCCTTCAGTCTTTTTCAGGCAGCAGACACG TCCCAGTCTGGAACCCTGGAAGGAGAAGAATTCGTACAGTTCTATAAGGCATTGACTAAACGTGCTGAG GTGCAGGAACTGTTTGAAAGTTTTTCAGCTGATGGGCAGAAGCTGACTCTGCTGGAATTTTTGGATTTC CTCCAAGAGGAGCAGAAGGAGAGAGACTGCACCTCTGAGCTTGCTCTGGAACTCATTGACCGCTATGAA CCTTCAGACAGTGGCAAACTGCGGCATGTGCTGAGTATGGATGGCTTCCTCAGCTACCTCTGCTCTAAG GATGGAGACATCTTCAACCCAGCCTGCCTCCCCATCTATCAGGATATGACTCAACCCCTGAACCACTAC TTCATCTGCTCTTCTCATAACACCTACCTAGTGGGGGACCAGCTTTGCGGCCAGAGCAGCGTCGAGGGA TATATACGGGCCCTGAAGCGGGGGTGCCGCTGCGTGGAGGTGGATGTATGGGATGGACCTAGCGGGGAA CCTGTCGTTTACCACGGACACACCCTGACCTCCCGCATCCTGTTCAAAGATGTCGTGGCCACAGTAGCA CAGTATGCCTTCCAGACATCAGACTACCCAGTCATCTTGTCCCTGGAGACCCACTGCAGCTGGGAGCAG CAGCAGACCATGGCCCGTCATCTGACTGAGATCCTGGGGGAGCAGCTGCTGAGCACCACCTTGGATGGG GTGCTGCCCACTCAGCTGCCCTCGCCTGAGGAGCTTCGGAGGAAGATCCTGGTGAAGGGGAAGAAGTTA ACACTTGAGGAAGACCTGGAATATGAGGAAGAGGAAGCAGAACCTGAGTTGGAAGAGTCAGAATTGGCG CTGGAGTCCCAGTTTGAGACTGAGCCTGAGCCCCAGGAGCAGAACCTTCAGAATAAGGACAAAAAGAAG AAATCCAAGCCCATCTTGTGTCCAGCCCTCTCTTCCCTGGTTATCTACTTGAAGTCTGTCTCATTCCGC AGCTTCACACATTCAAAGGAGCACTACCACTTCTACGAGATATCATCTTTCTCTGAAACCAAGGCCAAG CGCCTCATCAAGGAGGCTGGCAATGAGTTTGTGCAGCACAATACTTGGCAGTTAAGCCGTGTGTATCCC AGCGGCCTGAGGACAGACTCTTCCAACTACAACCCCCAGGAACTCTGGAATGCAGGCTGCCAGATGGTG GCCATGAATATGCAGACTGCAGGGCTTGAAATGGACATCTGTGATGGGCATTTCCGCCAGAATGGCGGC TGTGGCTATGTGCTGAAGCCAGACTTCCTGCGTGATATCCAGAGTTCTTTCCACCCTGAGAAGCCCATC AGCCCTTTCAAAGCCCAGACTCTCTTAATCCAGGTGATCAGCGGTCAGCAACTCCCCAAAGTGGACAAG ACCAAAGAGGGGTCCATTGTGGATCCACTGGTGAAAGTGCAGATCTTTGGCGTTCGTCTAGACACAGCA CGGCAGGAGACCAACTATGTGGAGAACAATGGTTTTAATCCATACTGGGGGCAGACACTATGTTTCCGG GTGCTGGTGCCTGAACTTGCCATGCTGCGTTTTGTGGTAATGGATTATGACTGGAAATCCCGAAATGAC TTTATTGGTCAGTACACCCTGCCTTGGACCTGCATGCAACAAGGTTACCGCCACATTCACCTGCTGTCC AAAGATGGCATCAGCCTCCGCCCAGCTTCCATCTTTGTGTATATCTGCATCCAGGAAGGCCTGGAGGGG GATGAGTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG202935
Blue=ORF Red=Cloning site Green=Tag(s) MASLLQDQLTTDQDLLLMQEGMSMRKVRSKSWKKLRYFRLQNDGMTVWHARQARGSAKPSFSISDVETI RNGHDSELLRSLAEELPLEQGFTIVFHGRRSNLDLMANSVEEAQIWMRGLQLLVDLVTSMDHQERLDQW LSDWFQRGDKNQDGKMSFQEVQRLLHLMNVEMDQEYAFSLFQAADTSQSGTLEGEEFVQFYKALTKRAE VQELFESFSADGQKLTLLEFLDFLQEEQKERDCTSELALELIDRYEPSDSGKLRHVLSMDGFLSYLCSK DGDIFNPACLPIYQDMTQPLNHYFICSSHNTYLVGDQLCGQSSVEGYIRALKRGCRCVEVDVWDGPSGE PVVYHGHTLTSRILFKDVVATVAQYAFQTSDYPVILSLETHCSWEQQQTMARHLTEILGEQLLSTTLDG VLPTQLPSPEELRRKILVKGKKLTLEEDLEYEEEEAEPELEESELALESQFETEPEPQEQNLQNKDKKK KSKPILCPALSSLVIYLKSVSFRSFTHSKEHYHFYEISSFSETKAKRLIKEAGNEFVQHNTWQLSRVYP SGLRTDSSNYNPQELWNAGCQMVAMNMQTAGLEMDICDGHFRQNGGCGYVLKPDFLRDIQSSFHPEKPI SPFKAQTLLIQVISGQQLPKVDKTKEGSIVDPLVKVQIFGVRLDTARQETNYVENNGFNPYWGQTLCFR VLVPELAMLRFVVMDYDWKSRNDFIGQYTLPWTCMQQGYRHIHLLSKDGISLRPASIFVYICIQEGLEG DES TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_032726 |
ORF Size | 2286 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 3107 bp |
RefSeq ORF | 2289 bp |
Locus ID | 84812 |
UniProt ID | Q9BRC7 |
Domains | C2, PI-PLC-X, PI-PLC-Y, PH, EFh |
Protein Families | Druggable Genome |
Protein Pathways | Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
MW | 87.6 kDa |
Gene Summary | This gene encodes a member of the delta class of phospholipase C enzymes. Phospholipase C enzymes play a critical role in many cellular processes by hydrolyzing phosphatidylinositol 4,5-bisphosphate into two intracellular second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. Expression of this gene may be a marker for cancer. [provided by RefSeq, Jan 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202935 | PLCD4 (Myc-DDK-tagged)-Human phospholipase C, delta 4 (PLCD4) |
CNY 5,584.00 |
|
RC202935L3 | Lenti ORF clone of Human phospholipase C, delta 4 (PLCD4), Myc-DDK-tagged |
CNY 7,700.00 |
|
RC202935L4 | Lenti ORF clone of Human phospholipase C, delta 4 (PLCD4), mGFP tagged |
CNY 7,700.00 |
|
SC320173 | PLCD4 (untagged)-Human phospholipase C, delta 4 (PLCD4) |
CNY 7,216.00 |