NSBP1 (HMGN5) (NM_030763) Human Tagged ORF Clone
CAT#: RG202766
- TrueORF®
HMGN5 (tGFP-tagged) - Human high mobility group nucleosome binding domain 5 (HMGN5). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_030763" in other vectors (2)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | NBP-45; NSBP1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG202766 ORF sequence, codon optimized.
Due to the complexity of NM_030763, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAAAACGAAAGGCTGCCGGTCAGGGGGACATGAGACAAGAGCCGAAACGCCGGTCCGCTAGGCTGA GTGCAATGCTGGTCCCGGTGACTCCCGAGGTGAAGCCTAAAAGAACTAGTAGTTCTCGGAAGATGAAGAC AAAAAGTGATATGATGGAGGAGAACATCGATACCTCCGCACAGGCCGTGGCCGAAACGAAGCAGGAGGCG GTCGTGGAAGAGGATTATAACGAGAATGCGAAAAATGGAGAAGCCAAGATCACCGAGGCTCCGGCTAGTG AAAAGGAGATCGTTGAAGTGAAAGAGGAAAATATCGAGGACGCCACAGAAAAGGGTGGAGAAAAGAAGGA GGCAGTTGCCGCCGAGGTTAAGAATGAAGAAGAGGACCAGAAAGAAGACGAAGAGGATCAGAACGAGGAA AAGGGCGAGGCAGGGAAGGAGGATAAAGACGAAAAGGGAGAAGAAGACGGAAAAGAAGATAAAAATGGCA ACGAGAAAGGGGAGGACGCCAAGGAAAAGGAGGATGGCAAGAAAGGAGAGGACGGGAAGGGGAATGGCGA AGACGGGAAAGAGAAGGGGGAGGATGAAAAGGAGGAGGAGGATCGCAAAGAGACGGGTGATGGCAAGGAG AACGAGGATGGGAAGGAAAAAGGCGACAAGAAAGAGGGAAAGGATGTGAAAGTAAAGGAAGATGAGAAAG AGAGAGAAGATGGCAAAGAAGACGAAGGTGGTAATGAGGAAGAAGCTGGCAAGGAGAAGGAAGACCTTAA AGAAGAGGAGGAGGGTAAAGAGGAGGACGAAATCAAGGAAGACGACGGAAAGAAGGAAGAACCTCAGAGT ATTGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202766 representing NM_030763
Red=Cloning site Green=Tags(s) MPKRKAAGQGDMRQEPKRRSARLSAMLVPVTPEVKPKRTSSSRKMKTKSDMMEENIDTSAQAVAETKQEA VVEEDYNENAKNGEAKITEAPASEKEIVEVKEENIEDATEKGGEKKEAVAAEVKNEEEDQKEDEEDQNEE KGEAGKEDKDEKGEEDGKEDKNGNEKGEDAKEKEDGKKGEDGKGNGEDGKEKGEDEKEEEDRKETGDGKE NEDGKEKGDKKEGKDVKVKEDEKEREDGKEDEGGNEEEAGKEKEDLKEEEEGKEEDEIKEDDGKKEEPQS IV TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_030763 |
ORF Size | 846 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_030763.2, NP_110390.1 |
RefSeq Size | 2126 bp |
RefSeq ORF | 849 bp |
Locus ID | 79366 |
UniProt ID | P82970 |
Domains | HMG14_17 |
Protein Families | Transcription Factors |
Gene Summary | This gene encodes a nuclear protein with similarities to the high mobility group proteins, HMG14 and HMG17, which suggests that this protein may function as a nucleosomal binding and transcriptional activating protein. [provided by RefSeq, Sep 2009] |
Documents
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