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PAR4 (PAWR) (NM_002583) Human Tagged ORF Clone
CAT＃: RG202733
- TrueORF®
PAWR (tGFP-tagged) - Human PRKC, apoptosis, WT1, regulator (PAWR)
ORF Plasmid: DDK
"NM_002583" in other vectors (7)
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CNY 5,256.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Par-4; PAR4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG202733 representing NM_002583
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCGGTGGCTACCGGACCAGCAGCGGCCTCGGCGGCAGCACCACAGACTTCCTGGAGGAGTGGA AGGCGAAACGCGAGAAGATGCGCGCCAAGCAGAACCCCCCGGGCCCGGCCCCCCCGGGAGGGGGCAGCAG CGACGCCGCTGGGAAGCCCCCCGCGGGGGCTCTGGGCACCCCGGCGGCCGCCGCTGCCAACGAGCTCAAC AACAACCTCCCGGGCGGCGCGCCGGCCGCACCTGCCGTCCCCGGTCCCGGGGGCGTGAACTGCGCGGTCG GCTCCGCCATGCTGACGCGGGCGGCCCCCGGCCCGCGGCGGTCGGAGGACGAGCCCCCAGCCGCCTCTGC CTCGGCTGCACCGCCGCCCCAGCGTGACGAGGAGGAGCCGGACGGCGTCCCAGAGAAGGGCAAGAGCTCG GGCCCCAGTGCCAGGAAAGGCAAGGGGCAGATCGAGAAGAGGAAGCTGCGGGAGAAGCGGCGCTCCACCG GCGTGGTCAACATCCCTGCCGCAGAGTGCTTAGATGAGTACGAAGATGATGAAGCAGGGCAGAAAGAGCG GAAACGAGAAGATGCAATTACACAACAGAACACTATTCAGAATGAAGCTGTAAACTTACTAGATCCAGGC AGTTCCTATCTGCTACAGGAGCCACCTAGAACAGTTTCAGGCAGATATAAAAGCACAACCAGTGTCTCTG AAGAAGATGTCTCAAGTAGATATTCTCGAACAGATAGAAGTGGGTTCCCTAGATATAACAGGGATGCAAA TGTTTCAGGTACTCTGGTTTCAAGTAGCACACTGGAAAAGAAAATTGAAGATCTTGAAAAGGAAGTAGTA AGAGAAAGACAAGAAAACCTAAGACTTGTGAGACTGATGCAAGATAAAGAGGAAATGATTGGAAAACTCA AAGAAGAAATTGATTTATTAAATAGAGACCTAGATGACATAGAAGATGAAAATGAACAGCTAAAGCAGGA AAATAAAACTCTTTTGAAAGTTGTGGGTCAGCTGACCAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202733 representing NM_002583
Red=Cloning site Green=Tags(s) MATGGYRTSSGLGGSTTDFLEEWKAKREKMRAKQNPPGPAPPGGGSSDAAGKPPAGALGTPAAAAANELN NNLPGGAPAAPAVPGPGGVNCAVGSAMLTRAAPGPRRSEDEPPAASASAAPPPQRDEEEPDGVPEKGKSS GPSARKGKGQIEKRKLREKRRSTGVVNIPAAECLDEYEDDEAGQKERKREDAITQQNTIQNEAVNLLDPG SSYLLQEPPRTVSGRYKSTTSVSEEDVSSRYSRTDRSGFPRYNRDANVSGTLVSSSTLEKKIEDLEKEVV RERQENLRLVRLMQDKEEMIGKLKEEIDLLNRDLDDIEDENEQLKQENKTLLKVVGQLTR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002583 |
| ORF Size | 1020 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002583.4 |
| RefSeq Size | 1967 bp |
| RefSeq ORF | 1023 bp |
| Locus ID | 5074 |
| UniProt ID | Q96IZ0 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | This gene encodes a tumor suppressor protein that selectively induces apoptosis in cancer cells through intracellular and extracellular mechanisms. The intracellular mechanism involves the inhibition of pro-survival pathways and the activation of Fas-mediated apoptosis, while the extracellular mechanism involves the binding of a secreted form of this protein to glucose regulated protein 78 (GRP78) on the cell surface, which leads to activation of the extrinsic apoptotic pathway. This gene is located on the unstable human chromosomal 12q21 region and is often deleted or mutated different tumors. The encoded protein also plays an important role in the progression of age-related diseases. [provided by RefSeq, Aug 2017] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Inhibition of AKT promotes FOXO3a-dependent apoptosis in prostate cancer
,null,
Cell Death & Disease
,PubMed ID 26913603
[PAWR]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202733 | PAWR (Myc-DDK-tagged)-Human PRKC, apoptosis, WT1, regulator (PAWR) |
CNY 3,656.00 |
|
| RC202733L1 | Lenti ORF clone of Human PRKC, apoptosis, WT1, regulator (PAWR), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC202733L2 | Lenti ORF clone of Human PRKC, apoptosis, WT1, regulator (PAWR), mGFP tagged |
CNY 5,890.00 |
|
| RC202733L3 | Lenti ORF clone of Human PRKC, apoptosis, WT1, regulator (PAWR), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC202733L4 | Lenti ORF clone of Human PRKC, apoptosis, WT1, regulator (PAWR), mGFP tagged |
CNY 6,056.00 |
|
| SC110969 | PAWR (untagged)-Human PRKC, apoptosis, WT1, regulator (PAWR) |
CNY 3,656.00 |
|
| SC320836 | PAWR (untagged)-Human PRKC, apoptosis, WT1, regulator (PAWR) |
CNY 3,656.00 |
