ISCA1 (NM_030940) Human Tagged ORF Clone
CAT#: RG201335
- TrueORF®
ISCA1 (tGFP-tagged) - Human iron-sulfur cluster assembly 1 homolog (S. cerevisiae) (ISCA1)
ORF Plasmid: DDK
"NM_030940" in other vectors (4)
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CNY 2,800.00
CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | HBLD2; hIscA; ISA1; MMDS5 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG201335 representing NM_030940
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCTTCCTTAGTCCGGGCAACTGTCCGGGCTGTGAGCAAGAGGAAGCTGCAGCCCACCCGGGCAG CCCTCACCCTGACACCTTCAGCAGTAAACAAGATAAAACAACTTCTTAAAGATAAGCCTGAGCATGTAGG TGTAAAAGTTGGTGTCCGAACCAGGGGCTGTAATGGCCTTTCTTATACTCTAGAATATACAAAGACAAAA GGAGATTCTGATGAAGAAGTTATTCAAGATGGAGTCAGAGTATTCATCGAAAAGAAAGCACAGCTAACAC TTTTAGGAACAGAAATGGACTATGTTGAAGACAAATTATCCAGTGAGTTTGTGTTCAATAACCCAAACAT CAAAGGGACTTGTGGCTGTGGAGAAAGCTTTAATATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201335 representing NM_030940
Red=Cloning site Green=Tags(s) MSASLVRATVRAVSKRKLQPTRAALTLTPSAVNKIKQLLKDKPEHVGVKVGVRTRGCNGLSYTLEYTKTK GDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYVEDKLSSEFVFNNPNIKGTCGCGESFNI TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_030940 |
ORF Size | 387 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_030940.4 |
RefSeq Size | 2012 bp |
RefSeq ORF | 390 bp |
Locus ID | 81689 |
UniProt ID | Q9BUE6 |
Domains | HesB-like |
Gene Summary | ISCA1 is a mitochondrial protein involved in the biogenesis and assembly of iron-sulfur clusters, which play a role in electron-transfer reactions (Cozar-Castellano et al., 2004 [PubMed 15262227]).[supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201335 | ISCA1 (Myc-DDK-tagged)-Human iron-sulfur cluster assembly 1 homolog (S. cerevisiae) (ISCA1) |
CNY 1,200.00 |
|
RC201335L3 | Lenti ORF clone of Human iron-sulfur cluster assembly 1 homolog (S. cerevisiae) (ISCA1), Myc-DDK-tagged |
CNY 3,600.00 |
|
RC201335L4 | Lenti ORF clone of Human iron-sulfur cluster assembly 1 homolog (S. cerevisiae) (ISCA1), mGFP tagged |
CNY 5,890.00 |
|
SC127008 | ISCA1 (untagged)-Human iron-sulfur cluster assembly 1 homolog (S. cerevisiae) (ISCA1) |
CNY 1,200.00 |