EPHA10 (NM_001099439) Human Tagged ORF Clone
CAT#: RC600047
- TrueORF®
EPHA10 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens EPH receptor A10, transcript variant 3, Signal peptide (1-33) plus EC domain (34-565)
"NM_001099439" in other vectors (7)
Need custom modification / cloning service?
Get a free quote
CNY 4,208.00
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | DDK-His |
Vector | pCMV6-XL5-DDK-His |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>RC600047 representing leader sequence plus the extracellular domain region of NM_001099439
Red=Cloning site Blue=ORF Green=Tags(s) GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG CGATCGCC ATGGAGACCTGCGCCGGTCCACACCCGCTGCGCCTCTTCCTCTGCCGGATGCAGCTCTGTCTCGCGCTGC TTTTGGGACCCTGGCGGCCTGGGACCGCCGAGGAAGTTATCCTCCTGGATTCCAAAGCCTCCCAGGCCGA GCTGGGCTGGACTGCACTGCCAAGTAATGGGTGGGAGGAGATCAGCGGCGTGGATGAACACGACCGTCCC ATCCGCACGTACCAAGTGTGCAATGTGCTGGAGCCCAACCAGGACAACTGGCTGCAGACTGGCTGGATAA GCCGTGGCCGCGGGCAGCGCATCTTCGTGGAACTGCAGTTCACACTCCGTGACTGCAGCAGCATCCCTGG CGCCGCGGGTACCTGCAAGGAGACCTTCAACGTCTACTACCTGGAAACTGAGGCCGACCTGGGCCGTGGG CGTCCCCGCCTAGGCGGCAGCCGGCCCCGCAAAATCGACACGATCGCGGCGGACGAGAGCTTCACGCAGG GCGACCTGGGTGAGCGCAAGATGAAGCTGAACACAGAGGTGCGCGAGATCGGACCGCTCAGCCGGCGGGG TTTCCACCTGGCCTTTCAGGACGTGGGCGCATGCGTGGCGCTTGTCTCGGTGCGCGTCTACTACAAGCAG TGCCGCGCCACCGTGCGGGGCCTGGCCACGTTCCCAGCCACCGCAGCCGAGAGCGCCTTCTCCACACTGG TGGAAGTGGCCGGAACGTGCGTGGCGCACTCGGAAGGGGAGCCTGGCAGCCCCCCACGCATGCACTGCGG CGCCGACGGCGAGTGGCTGGTGCCTGTGGGCCGCTGCAGCTGCAGCGCGGGATTCCAGGAGCGTGGTGAC TTCTGCGAAGCCTGTCCCCCAGGGTTTTACAAGGTGTCCCCGCGGCGGCCCCTCTGCTCACCGTGCCCAG AGCACAGCCGGGCCCTGGAAAACGCCTCCACCTTCTGCGTGTGCCAGGACAGCTATGCGCGCTCACCCAC CGACCCGCCCTCGGCTTCCTGCACCCGGCCGCCGTCGGCGCCGCGGGACCTGCAGTACAGCCTGAGCCGC TCGCCGCTGGTGCTGCGACTGCGCTGGCTGCCGCCGGCCGACTCGGGAGGCCGCTCGGACGTCACCTACT CGCTGCTGTGCCTGCGCTGCGGCCGCGAGGGCCCGGCGGGCGCCTGCGAGCCGTGCGGGCCGCGCGTGGC CTTCCTACCGCGCCAGGCAGGGCTGCGGGAGCGAGCCGCCACGCTGCTGCACCTGCGGCCCGGCGCGCGC TACACCGTGCGCGTGGCCGCGCTCAACGGCGTCTCGGGCCCGGCGGCCGCCGCGGGAACCACCTACGCGC AGGTCACCGTCTCCACCGGGCCCGGGGCGCCCTGGGAGGAGGATGAGATCCGCAGGGACCGAGTGGAACC CCAGAGCGTGTCCCTGTCGTGGCGGGAGCCCATCCCTGCCGGAGCCCCTGGGGCCAATGACACGGAGTAC GAGATCCGATACTACGAGAAGGGTCAGAGTGAGCAGACTTACTCCATGGTGAAGACAGGGGCGCCCACAG TCACCGTCACCAACCTGAAGCCGGCTACCCGCTACGTCTTTCAGATCCGGGCCGCTTCCCCGGGGCCATC CTGGGAGGCCCAGAGTTTTAACCCCAGCATTGAAGTACAGACCCTGGGGGAGGCTGCCTCAGGGTCCAGG GACCAGAGCCCCGCC ACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATTAATGAGATC TGGTACCGATATCAAGCTTGTCGACTCTAGA >RC600047 representing signal peptide plus the extracellular domain region of NM_001099439
Red=Cloning sites Green= DDK and 6XHIS Tags METCAGPHPLRLFLCRMQLCLALLLGPWRPGTAEEVILLDSKASQAELGWTALPSNGWEEISGVDEHDRP IRTYQVCNVLEPNQDNWLQTGWISRGRGQRIFVELQFTLRDCSSIPGAAGTCKETFNVYYLETEADLGRG RPRLGGSRPRKIDTIAADESFTQGDLGERKMKLNTEVREIGPLSRRGFHLAFQDVGACVALVSVRVYYKQ CRATVRGLATFPATAAESAFSTLVEVAGTCVAHSEGEPGSPPRMHCGADGEWLVPVGRCSCSAGFQERGD FCEACPPGFYKVSPRRPLCSPCPEHSRALENASTFCVCQDSYARSPTDPPSASCTRPPSAPRDLQYSLSR SPLVLRLRWLPPADSGGRSDVTYSLLCLRCGREGPAGACEPCGPRVAFLPRQAGLRERAATLLHLRPGAR YTVRVAALNGVSGPAAAAGTTYAQVTVSTGPGAPWEEDEIRRDRVEPQSVSLSWREPIPAGAPGANDTEY EIRYYEKGQSEQTYSMVKTGAPTVTVTNLKPATRYVFQIRAASPGPSWEAQSFNPSIEVQTLGEAASGSR DQSPA TRSGTRSGDYKDDDDKGSHHHHHH |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_001099439 |
ORF Size | 1695 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the extra cellular domain of the protein with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001099439.1, NP_001092909.1 |
RefSeq Size | 5526 bp |
RefSeq ORF | 3027 bp |
Locus ID | 284656 |
UniProt ID | Q5JZY3 |
Protein Families | Druggable Genome, Protein Kinase |
MW | 61.3 kDa |
Gene Summary | Ephrin receptors, the largest subfamily of receptor tyrosine kinases (RTKs), and their ephrin ligands are important mediators of cell-cell communication regulating cell attachment, shape, and mobility in neuronal and epithelial cells (Aasheim et al., 2005 [PubMed 15777695]). See MIM 179610 for additional background on Eph receptors and ephrins.[supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC218374 | EPHA10 (Myc-DDK-tagged)-Human EPH receptor A10 (EPHA10), transcript variant 3 |
CNY 7,016.00 |
|
RC218374L1 | Lenti ORF clone of Human EPH receptor A10 (EPHA10), transcript variant 3, Myc-DDK-tagged |
CNY 9,416.00 |
|
RC218374L2 | Lenti ORF clone of Human EPH receptor A10 (EPHA10), transcript variant 3, mGFP tagged |
CNY 9,416.00 |
|
RC218374L3 | Lenti ORF clone of Human EPH receptor A10 (EPHA10), transcript variant 3, Myc-DDK-tagged |
CNY 9,416.00 |
|
RC218374L4 | Lenti ORF clone of Human EPH receptor A10 (EPHA10), transcript variant 3, mGFP tagged |
CNY 9,416.00 |
|
RG218374 | EPHA10 (tGFP-tagged) - Human EPH receptor A10 (EPHA10), transcript variant 3 |
CNY 11,120.00 |
|
SC316583 | EPHA10 (untagged)-Human EPH receptor A10 (EPHA10), transcript variant 3 |
CNY 7,024.00 |