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IGF1 Receptor (IGF1R) (NM_000875) Human Tagged ORF Clone
CAT＃: RC600009
- TrueORF®
IGF1R (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens insulin-like growth factor 1 receptor, Signal peptide (1-30) plus EC domain (741-935)
"NM_000875" in other vectors (7)
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CNY 9,360.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | DDK-His |
| Synonyms | CD221; IGFIR; IGFR; JTK13 |
| Vector | pCMV6-XL5-DDK-His |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>RC600009 representing leader sequence plus the extracellular domain region of NM_000875
Red=Cloning site Blue=ORF Green=Tags(s) GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG CGATCGCC ATGAAGTCTGGCTCCGGAGGAGGGTCCCCGACCTCGCTGTGGGGGCTCCTGTTTCTCTCCGCCGCGCTCT CGCTCTGGCCGACGAGTGGAGATGTCATGCAAGTGGCCAACACCACCATGTCCAGCCGAAGCAGGAACAC CACGGCCGCAGACACCTACAACATCACCGACCCGGAAGAGCTGGAGACAGAGTACCCTTTCTTTGAGAGC AGAGTGGATAACAAGGAGAGAACTGTCATTTCTAACCTTCGGCCTTTCACATTGTACCGCATCGATATCC ACAGCTGCAACCACGAGGCTGAGAAGCTGGGCTGCAGCGCCTCCAACTTCGTCTTTGCAAGGACTATGCC CGCAGAAGGAGCAGATGACATTCCTGGGCCAGTGACCTGGGAGCCAAGGCCTGAAAACTCCATCTTTTTA AAGTGGCCGGAACCTGAGAATCCCAATGGATTGATTCTAATGTATGAAATAAAATACGGATCACAAGTTG AGGATCAGCGAGAATGTGTGTCCAGACAGGAATACAGGAAGTATGGAGGGGCCAAGCTAAACCGGCTAAA CCCGGGGAACTACACAGCCCGGATTCAGGCCACATCTCTCTCTGGGAATGGGTCGTGGACAGATCCTGTG TTCTTCTATGTCCAGGCCAAAACAGGATATGAAAACTTCATCCAT ACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATTAATGAGATC TGGTACCGATATCAAGCTTGTCGACTCTAGA >RC600009 representing signal peptide plus the extracellular domain region of NM_000875
Red=Cloning sites Green= DDK and 6XHIS Tags MKSGSGGGSPTSLWGLLFLSAALSLWPTSGDVMQVANTTMSSRSRNTTAADTYNITDPEELETEYPFFES RVDNKERTVISNLRPFTLYRIDIHSCNHEAEKLGCSASNFVFARTMPAEGADDIPGPVTWEPRPENSIFL KWPEPENPNGLILMYEIKYGSQVEDQRECVSRQEYRKYGGAKLNRLNPGNYTARIQATSLSGNGSWTDPV FFYVQAKTGYENFIH TRSGTRSGDYKDDDDKGSHHHHHH |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_000875 |
| ORF Size | 4104 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the extra cellular domain of the protein with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000875.3, NP_000866.1 |
| RefSeq Size | 12235 bp |
| RefSeq ORF | 4104 bp |
| Locus ID | 3480 |
| UniProt ID | P08069 |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Adherens junction, Colorectal cancer, Endocytosis, Focal adhesion, Glioma, Long-term depression, Melanoma, Oocyte meiosis, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer |
| MW | 25.2 kDa |
| Gene Summary | This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214928 | IGF1R (Myc-DDK-tagged)-Human insulin-like growth factor 1 receptor (IGF1R) |
CNY 14,032.00 |
|
| RC214928L1 | Lenti ORF clone of Human insulin-like growth factor 1 receptor (IGF1R), Myc-DDK-tagged |
CNY 16,432.00 |
|
| RC214928L2 | Lenti ORF clone of Human insulin-like growth factor 1 receptor (IGF1R), mGFP tagged |
CNY 16,432.00 |
|
| RC214928L3 | Lenti ORF clone of Human insulin-like growth factor 1 receptor (IGF1R), Myc-DDK-tagged |
CNY 16,432.00 |
|
| RC214928L4 | Lenti ORF clone of Human insulin-like growth factor 1 receptor (IGF1R), mGFP tagged |
CNY 16,432.00 |
|
| RG214928 | IGF1R (tGFP-tagged) - Human insulin-like growth factor 1 receptor (IGF1R) |
CNY 15,632.00 |
|
| SC124152 | IGF1R (untagged)-Human insulin-like growth factor 1 receptor (IGF1R) |
CNY 14,040.00 |
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