JMJD1C (NM_001282948) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	KDM3C; TRIP-8; TRIP8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240245 representing NM_001282948 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAGGTCCTTATTCCTTAAATGGATACAGAGTGAGAGTATATAGACAAGACTCTGCCACCCAGTGGT TTACTGGCATAATTACTCATCATGATCTCTTCACCCGCACCATGATCGTTATGAATGATCAGGTACTAGA ACCACAGAATGTCGATCCTTCTATGGTTCAAATGACCTTTCTAGATGATGTTGTTCACTCTTTGTTAAAA GGTGAAAATATTGGCATTACATCACGACGCAGGTCTCGTGCCAATCAAAACGTCAACGCTGTTCACAGCC ATTATACACGTGCCCAAGCAAATAGTCCCAGACCAGCAATGAACTCCCAAGCTGCTGTACCAAAACAGAA TACACACCAGCAACAGCAACAAAGAAGTATCCGTCCAAATAAGAGGAAGGGCTCAGATAGCAGTATACCA GATGAAGAGAAGATGAAGGAGGAAAAATATGATTATATATCACGAGGAGAAAATCCTAAAGGTAAAAACA AACACTTGATGAATAAAAGAAGGAAACCTGAGGAGGATGAAAAGAAACTAAATATGAAAAGACTTCGAAC TGACAATGTTTCAGACTTTTCTGAGAGCAGTGACTCAGAAAATTCAAATAAGAGAATAATAGATAATTCC TCAGAACAGAAGCCAGAGAATGAATTGAAAAATAAAAATACTTCAAAAATAAATGGAGAAGAAGGAAAAC CCCATAATAATGAGAAGGCAGGAGAAGAGACCCTAAAAAATAGCCAGCCTCCCTGGGATCAAATACAGGA AGATAAAAAACATGAAGAAGCAGAGAAGCGGAAGTCTGTTGACACTCAGCTTCAAGAAGATATGATTATT CATTCGTCAGAACAGTCCACAGTTTCTGATCATAATTCTAATGATTTACTTCCTCAGGAATGCAATATGG ATAAAACACATACCATGGAATTGCTACCAAAGGAGAAGTTTGTATCCAGACCACCCACACCAAAATGTGT TATTGATATTACAAATGACACTAATTTAGAAAAGGTGGCTCAGGAAAACTCAAGTACCTTTGGCCTTCAG ACACTTCAGAAAATGGATCCTAATGTTAGTGATTCAAAACACTCTATTGCAAATGCAAAATTCTTGGAAA CAGCAAAAAAAGATTCTGACCAGAGCTGGGTCAGTGATGTAGTTAAAGTGGATCTAACCCAATCAAGTGT TACAAATGCTTCTTCAGGAAATGATCACTTGAACATGGAAAAAGAGAAGTATGTCTCTTACATTTCTCCT TTAAGTGCAGTTTCTGTCATGGAAGATAAGCTGCATAAGCGAAGTCCACCTCCAGAGACTATAAAATCTA AACTTAATACTTCAGTAGATACTCACAAGATAAAATCCAGCCCATCACCTGAAGTTGTTAAACCCAAAAT AACTCATTCTCCTGATTCTGTAAAGTCTAAGGCCACTTATGTGAACAGCCAAGCTACTGGTGAAAGAAGA TTGGCAAATAAGATAGAACATGAGCTATCAAGATGCAGTTTTCATCCAATTCCTACTCGAAGCAGTACAT TAGAAACTACAAAGAGTCCTCTTATCATTGATAAAAATGAGCATTTTACAGTTTACAGAGATCCTGCACT TATTGGGTCAGAAACAGGAGCTAATCATATTTCACCTTTCCTAAGCCAGCATCCTTTTCCTCTTCACTCC TCATCTCATAGAACCTGTTTAAATCCAGGTACCCATCATCCTGCCTTAACTCCTGCACCCCATTTACTAG CCGGATCATCTAGTCAAACTCCATTACCTACCATTAACACTCATCCTCTGACTAGTGGTCCACACCATGC TGTTCATCACCCTCATTTACTTCCCACTGTGTTACCTGGAGTGCCTACTGCCTCCTTACTTGGTGGCCAC CCACGACTAGAGAGTGCTCATGCCAGCAGCTTGAGCCACTTAGCGCTAGCACACCAGCAACAACAACAGT TGTTACAGCACCAGTCACCTCATCTTCTTGGACAAGCCCATCCTTCTGCTTCATATAATCAGCTTGGACT TTATCCAATTATTTGGCAGTATCCAAATGGAACACATGCATACTCAGGACTTGGTTTGCCTTCTTCTAAG TGGGTTCACCCAGAAAATGCAGTTAATGCTGAAGCTTCATTAAGGAGGAATTCTCCCAGTCCTTGGCTAC ATCAGCCCACCCCTGTGACCTCAGCAGATGGTATTGGATTACTTAGTCACATTCCTGTCAGACCTTCCAG TGCAGAGCCTCATCGGCCTCTTAAAATTACAGCCCATTCCAGTCCACCATTGACAAAAACTTTAGTAGAT CATCATAAGGAAGAATTAGAAAGAAAAGCTTTTATGGAACCATTACGGTCTGTTGCATCCACATCAGCCA AAAATGACCTGGATCTAAATAGGTCACAGACTGGAAAAGATTGTCACTTACATAGGCATTTTGTGGATCC AGTATTAAATCAGTTACAGAGGCCACCCCAGGAGACTGGAGAGAGGTTAAACAAATACAAAGAGGAACAC CGTCGAATTCTTCAAGAAAGTATTGATGTTGCTCCCTTTACAACTAAAATCAAGGGACTTGAGGGTGAGA GAGAGAATTATTCCAGAGTGGCATCATCATCTTCCAGTCCTAAAAGCCATATCATCAAACAAGATATGGA TGTAGAACGCTCAGTATCAGATCTTTATAAAATGAAGCACTCAGTGCCTCAGAGTTTACCCCAAAGTAAC TATTTCACTACATTGTCTAATAGTGTGGTCAATGAACCACCAAGATCATACCCATCCAAAGAAGTTTCAA ATATTTACGGTGATAAACAGAGTAATGCCCTTGCAGCGGCAGCAGCTAATCCTCAAACTCTGACTTCATT TATAACATCTCTTTCAAAGCCTCCACCTTTGATTAAACACCAACCAGAAAGTGAAGGTTTAGTAGGCAAG ATACCAGAACATCTTCCACATCAGATTGCATCTCACTCAGTAACAACCTTCAGAAATGATTGTAGGAGTC CTACCCATTTGACAGTTTCTTCTACAAATACACTCCGCAGTATGCCTGCATTACATAGAGCACCAGTATT TCACCCACCAATCCATCACAGCCTGGAAAGAAAGGAAGGCAGCTATAGTAGTCTTTCCCCTCCAACTTTA ACTCCGGTGATGCCAGTAAATGCTGGTGGTAAAGTTCAAGAATCACAGAAGCCTCCAACTCTAATACCCG AACCAAAAGACTCCCAGGCAAATTTTAAGAGTTCTTCAGAACAGAGTTTGACGGAGATGTGGAGACCTAA TAATAACCTCAGCAAAGAGAAAACTGAATGGCATGTGGAGAAAAGCAGCGGAAAGTTACAGGCTGCTATG GCATCTGTCATTGTGCGTCCATCTTCTAGTACAAAAACTGATAGTATGCCAGCAATGCAGTTAGCTTCTA AAGATCGAGTTAGTGAAAGATCTTCAGCTGGGGCACATAAAACAGATTGCCTCAAACTAGCAGAAGCCGG AGAAACTGGAAGAATCATTTTGCCAAATGTGAATTCAGACAGTGTTCACACAAAATCTGAAAAAAACTTT CAGGCTGTCTCACAGGGCAGTGTTCCCAGTTCAGTCATGTCTGCTGTAAATACGATGTGTAATACCAAAA CGGATGTAATCACATCTGCTGCCGATACTACCAGTGTTTCCAGCTGGGGTGGTTCAGAAGTAATTTCCTC TTTATCAAATACCATTTTGGCCTCTACATCATCAGAATGTGTATCTTCAAAAAGTGTCAGTCAGCCAGTG GCTCAAAAACAAGAATGCAAGGTCAGCACCACAGCACCAGTTACATTAGCCAGTAGTAAGACAGGAAGTG TTGTTCAACCCAGTTCTGGGTTCTCAGGCACAACTGATTTTATCCATTTAAAAAAGCACAAGGCAGCATT GGCTGCAGCTCAGTATAAAAGTAGTAATGCCAGTGAGACTGAACCTAATGCTATAAAAAATCAGACACTT TCAGCCTCCCTTCCTCTGGATAGCACTGTAATCTGTAGTACAATTAACAAAGCAAACTCTGTAGGAAATG GGCAAGCTTCCCAGACAAGTCAACCAAACTACCATACTAAACTGAAAAAGGCCTGGCTCACCAGACACTC AGAAGAAGATAAAAATACTAATAAAATGGAAAATTCAGGGAATTCTGTATCAGAAATTATTAAGCCATGT TCTGTCAACTTAATAGCCTCTACATCTAGTGATATACAAAATAGTGTAGATAGTAAGATCATAGTTGATA AATATGTAAAAGATGATAAAGTCAACAGGAGAAAAGCCAAAAGAACTTATGAATCTGGCTCTGAAAGTGG AGACTCAGATGAAAGTGAAAGCAAGTCAGAGCAAAGGACTAAAAGACAACCTAAGCCAACTTACAAAAAG AAGCAAAATGATTTGCAAAAGAGAAAAGGTGAAATAGAAGAAGATTTGAAACCCAATGGAGTTCTCAGCA GGAGTGCCAAAGAAAGAAGTAAACTGAAGTTGCAAAGCAACAGTAATACTGGCATTCCTCGTTCAGTATT GAAAGATTGGCGTAAAGTCAAGAAGCTGAAGCAAACTGGGGAATCCTTTTTACAGGATGACTCCTGCTGT GAGATAGGGCCTAATTTACAAAAGTGTCGAGAATGTAGACTTATTCGCAGTAAAAAAGGAGAAGAACCAG CTCACTCACCAGTATTTTGTAGATTTTACTACTTTAGACGGTTGTCATTTAGTAAAAACGGAGTAGTTAG AATAGATGGTTTCTCTTCTCCTGACCAATATGATGATGAAGCTATGAGTTTGTGGACACATGAAAATTTT GAAGATGATGAACTAGATATAGAGACTTCTAAATATATCTTGGATATAATAGGTGATAAGTTCTGTCAAT TAGTAACATCTGAAAAAACAGCTTTGTCCTGGGTGAAAAAGGATGCCAAAATTGCCTGGAAAAGAGCAGT GAGAGGAGTCCGGGAGATGTGTGATGCATGTGAAGCAACATTGTTTAACATTCACTGGGTCTGCCAAAAA TGTGGATTTGTGGTCTGCTTAGATTGTTACAAGGCAAAGGAAAGGAAGAGTTCTAGAGATAAAGAACTAT ATGCTTGGATGAAGTGTGTGAAGGGACAGCCTCATGATCACAAACATTTAATGCCAACCCAAATTATACC TGGTTCTGTTTTGACAGATCTTCTAGATGCCATGCACACTCTTAGGGAAAAATATGGTATTAAATCCCAT TGTCATTGTACTAACAAACAGAATTTACAAGTTGGAAATTTTCCTACAATGAATGGTGTATCTCAAGTTT TACAGAATGTTCTTAATCACAGTAATAAAATTTCTCTGTGCATGCCTGAGTCTCAGCAGCAAAATACTCC TCCGAAGTCTGAGAAAAATGGTGGCAGCAGCCCAGAGAGTGATGTAGGCACAGATAACAAGTTAACTCCT CCAGAATCCCAGTCACCACTGCACTGGTTAGCAGATCTTGCAGAGCAAAAAGCCAGAGAGGAAAAAAAAG AAAACAAAGAACTTACCCTTGAAAACCAAATTAAAGAAGAAAGAGAACAAGACAACTCTGAATCTCCAAA TGGCAGAACATCACCTCTTGTGTCCCAGAATAATGAACAAGGCTCAACCTTACGGGATTTGCTGACTACA ACAGCTGGAAAGCTACGTGTGGGGTCTACAGATGCTGGCATTGCCTTTGCCCCAGTATATTCAATGGGAG CCCCAAGTAGCAAAAGTGGACGGACTATGCCTAACATTCTTGATGACATAATTGCTTCAGTTGTTGAAAA CAAAATTCCACCAAGTAAAACCTCCAAGATAAATGTAAAACCAGAGCTTAAAGAAGAGCCTGAAGAAAGC ATAATATCTGCAGTGGATGAAAATAATAAATTATACAGTGATATACCACATTCTTGGATCTGTGAGAAGC ATATTTTATGGCTTAAGGATTATAAGAATAGCAGTAATTGGAAGCTTTTCAAAGAATGTTGGAAACAAGG ACAGCCTGCAGTGGTTTCTGGTGTGCATAAGAAAATGAACATTAGCCTATGGAAGGCGGAATCAATTAGT CTTGATTTTGGAGACCACCAAGCTGATCTCCTGAACTGCAAAGATAGCATCATTTCAAATGCCAATGTTA AGGAATTCTGGGATGGTTTTGAAGAAGTTTCAAAACGGCAGAAAAACAAGAGTGGAGAAACAGTTGTTTT AAAATTGAAAGACTGGCCTTCAGGAGAAGACTTCAAGACTATGATGCCAGCAAGATACGAAGATCTTTTA AAAAGTCTGCCATTGCCAGAATATTGTAATCCAGAAGGAAAATTCAATTTGGCCTCTCATTTGCCAGGAT TTTTTGTACGTCCTGATCTAGGACCCAGGTTGTGCAGTGCCTATGGTGTAGTTGCTGCTAAAGATCATGA TATAGGAACAACAAATCTCCATATTGAAGTTTCTGATGTTGTAAATATACTAGTTTATGTTGGCATAGCA AAAGGAAATGGCATTCTCTCAAAAGCAGGAATTCTCAAGAAATTTGAGGAAGAAGATTTGGATGACATTT TAAGGAAAAGATTGAAGGACTCAAGTGAAATACCTGGTGCTCTGTGGCATATTTATGCTGGGAAAGATGT TGACAAGATAAGGGAATTTCTTCAAAAGATTTCAAAAGAACAAGGCCTTGAAGTTCTACCAGAACATGAT CCAATACGTGACCAAAGTTGGTATGTGAACAAAAAGCTCCGTCAAAGGCTGCTTGAAGAATATGGAGTCA GAACCTGTACTCTTATTCAGTTCCTTGGTGATGCTATTGTTTTGCCAGCGGGAGCACTTCATCAGGTTCA GAATTTTCACAGCTGTATTCAGGTAACTGAAGATTTTGTGTCTCCAGAACATCTTGTAGAGTCATTTCAT TTAACACAGGAACTGAGACTTTTGAAGGAAGAAATCAATTATGATGATAAACTACAGGTTAAAAATATTT TGTATCATGCAGTCAAAGAAATGGTGAGAGCCTTGAAGATACACGAGGATGAAGTAGAGGATATGGAAGA AAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240245 representing NM_001282948 Red=Cloning site Green=Tags(s) MQGPYSLNGYRVRVYRQDSATQWFTGIITHHDLFTRTMIVMNDQVLEPQNVDPSMVQMTFLDDVVHSLLK GENIGITSRRRSRANQNVNAVHSHYTRAQANSPRPAMNSQAAVPKQNTHQQQQQRSIRPNKRKGSDSSIP DEEKMKEEKYDYISRGENPKGKNKHLMNKRRKPEEDEKKLNMKRLRTDNVSDFSESSDSENSNKRIIDNS SEQKPENELKNKNTSKINGEEGKPHNNEKAGEETLKNSQPPWDQIQEDKKHEEAEKRKSVDTQLQEDMII HSSEQSTVSDHNSNDLLPQECNMDKTHTMELLPKEKFVSRPPTPKCVIDITNDTNLEKVAQENSSTFGLQ TLQKMDPNVSDSKHSIANAKFLETAKKDSDQSWVSDVVKVDLTQSSVTNASSGNDHLNMEKEKYVSYISP LSAVSVMEDKLHKRSPPPETIKSKLNTSVDTHKIKSSPSPEVVKPKITHSPDSVKSKATYVNSQATGERR LANKIEHELSRCSFHPIPTRSSTLETTKSPLIIDKNEHFTVYRDPALIGSETGANHISPFLSQHPFPLHS SSHRTCLNPGTHHPALTPAPHLLAGSSSQTPLPTINTHPLTSGPHHAVHHPHLLPTVLPGVPTASLLGGH PRLESAHASSLSHLALAHQQQQQLLQHQSPHLLGQAHPSASYNQLGLYPIIWQYPNGTHAYSGLGLPSSK WVHPENAVNAEASLRRNSPSPWLHQPTPVTSADGIGLLSHIPVRPSSAEPHRPLKITAHSSPPLTKTLVD HHKEELERKAFMEPLRSVASTSAKNDLDLNRSQTGKDCHLHRHFVDPVLNQLQRPPQETGERLNKYKEEH RRILQESIDVAPFTTKIKGLEGERENYSRVASSSSSPKSHIIKQDMDVERSVSDLYKMKHSVPQSLPQSN YFTTLSNSVVNEPPRSYPSKEVSNIYGDKQSNALAAAAANPQTLTSFITSLSKPPPLIKHQPESEGLVGK IPEHLPHQIASHSVTTFRNDCRSPTHLTVSSTNTLRSMPALHRAPVFHPPIHHSLERKEGSYSSLSPPTL TPVMPVNAGGKVQESQKPPTLIPEPKDSQANFKSSSEQSLTEMWRPNNNLSKEKTEWHVEKSSGKLQAAM ASVIVRPSSSTKTDSMPAMQLASKDRVSERSSAGAHKTDCLKLAEAGETGRIILPNVNSDSVHTKSEKNF QAVSQGSVPSSVMSAVNTMCNTKTDVITSAADTTSVSSWGGSEVISSLSNTILASTSSECVSSKSVSQPV AQKQECKVSTTAPVTLASSKTGSVVQPSSGFSGTTDFIHLKKHKAALAAAQYKSSNASETEPNAIKNQTL SASLPLDSTVICSTINKANSVGNGQASQTSQPNYHTKLKKAWLTRHSEEDKNTNKMENSGNSVSEIIKPC SVNLIASTSSDIQNSVDSKIIVDKYVKDDKVNRRKAKRTYESGSESGDSDESESKSEQRTKRQPKPTYKK KQNDLQKRKGEIEEDLKPNGVLSRSAKERSKLKLQSNSNTGIPRSVLKDWRKVKKLKQTGESFLQDDSCC EIGPNLQKCRECRLIRSKKGEEPAHSPVFCRFYYFRRLSFSKNGVVRIDGFSSPDQYDDEAMSLWTHENF EDDELDIETSKYILDIIGDKFCQLVTSEKTALSWVKKDAKIAWKRAVRGVREMCDACEATLFNIHWVCQK CGFVVCLDCYKAKERKSSRDKELYAWMKCVKGQPHDHKHLMPTQIIPGSVLTDLLDAMHTLREKYGIKSH CHCTNKQNLQVGNFPTMNGVSQVLQNVLNHSNKISLCMPESQQQNTPPKSEKNGGSSPESDVGTDNKLTP PESQSPLHWLADLAEQKAREEKKENKELTLENQIKEEREQDNSESPNGRTSPLVSQNNEQGSTLRDLLTT TAGKLRVGSTDAGIAFAPVYSMGAPSSKSGRTMPNILDDIIASVVENKIPPSKTSKINVKPELKEEPEES IISAVDENNKLYSDIPHSWICEKHILWLKDYKNSSNWKLFKECWKQGQPAVVSGVHKKMNISLWKAESIS LDFGDHQADLLNCKDSIISNANVKEFWDGFEEVSKRQKNKSGETVVLKLKDWPSGEDFKTMMPARYEDLL KSLPLPEYCNPEGKFNLASHLPGFFVRPDLGPRLCSAYGVVAAKDHDIGTTNLHIEVSDVVNILVYVGIA KGNGILSKAGILKKFEEEDLDDILRKRLKDSSEIPGALWHIYAGKDVDKIREFLQKISKEQGLEVLPEHD PIRDQSWYVNKKLRQRLLEEYGVRTCTLIQFLGDAIVLPAGALHQVQNFHSCIQVTEDFVSPEHLVESFH LTQELRLLKEEINYDDKLQVKNILYHAVKEMVRALKIHEDEVEDMEEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282948
ORF Size	7074 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282948.1, NP_001269877.1
RefSeq Size	8675 bp
RefSeq ORF	7077 bp
Locus ID	221037
UniProt ID	Q15652
Protein Families	Druggable Genome
MW	263.6 kDa
Gene Summary	The protein encoded by this gene interacts with thyroid hormone receptors and contains a jumonji domain. It is a candidate histone demethylase and is thought to be a coactivator for key transcription factors. It plays a role in the DNA-damage response pathway by demethylating the mediator of DNA damage checkpoint 1 (MDC1) protein, and is required for the survival of acute myeloid leukemia. Mutations in this gene are associated with Rett syndrome and intellectual disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

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