KDM6A (NM_001291415) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	bA386N14.2; KABUK2; UTX
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240152 representing NM_001291415 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATCCTGCGGAGTGTCGCTCGCTACCGCCGCCGCTGCCGCCGCCGCTTTCGGTGATGAGGAAAAGA AAATGGCGGCGGGAAAAGCGAGCGGCGAGAGCGAGGAGGCGTCCCCCAGCCTGACAGCCGAGGAGAGGGA GGCGCTCGGCGGACTGGACAGCCGCCTCTTTGGGTTCGTGAGATTTCATGAAGATGGCGCCAGGACGAAG GCCCTACTGGGCAAGGCTGTTCGCTGCTATGAATCTCTAATCTTAAAAGCTGAAGGAAAAGTGGAGTCTG ATTTCTTTTGTCAATTAGGTCACTTCAACCTCTTATTGGAAGATTATCCAAAAGCATTATCTGCATACCA GAGGTACTACAGTTTACAGTCTGACTACTGGAAGAATGCTGCCTTTTTATATGGTCTTGGTTTGGTCTAC TTCCATTATAATGCATTTCAGTGGGCAATTAAAGCATTTCAGGAGGTGCTTTATGTTGATCCCAGCTTTT GTCGAGCCAAGGAAATTCATTTACGACTTGGGCTTATGTTCAAAGTGAACACAGACTATGAGTCTAGTTT AAAGCATTTTCAGTTAGCTTTGGTTGACTGTAATCCCTGCACTTTGTCCAATGCTGAAATTCAATTTCAC ATTGCCCACTTATATGAAACCCAGAGGAAATATCATTCTGCAAAAGAAGCTTATGAACAACTTTTGCAGA CAGAGAATCTTTCTGCACAAGTAAAAGCAACTGTCTTACAACAGTTAGGTTGGATGCATCACACTGTAGA TCTCCTGGGAGATAAAGCCACCAAGGAAAGCTATGCTATTCAGTATCTCCAAAAGTCCTTGGAAGCAGAT CCTAATTCTGGCCAGTCCTGGTATTTCCTCGGAAGGTGCTATTCAAGTATTGGGAAAGTTCAGGATGCCT TTATATCTTACAGGCAGTCTATTGATAAATCAGAAGCAAGTGCAGATACATGGTGTTCAATAGGTGTGCT ATATCAGCAGCAAAATCAGCCCATGGATGCTTTACAGGCCTATATTTGTGCTGTACAATTGGACCATGGC CATGCTGCAGCCTGGATGGACCTAGGCACTCTCTATGAATCCTGCAACCAGCCTCAGGATGCCATTAAAT GCTACTTAAATGCAACTAGAAGCAAAAGTTGTAGTAATACCTCTGCACTTGCAGCACGAATTAAGTATTT ACAGGCTCAGTTGTGTAACCTTCCACAAGGTAGTCTACAGAATAAAACTAAATTACTTCCTAGTATTGAG GAGGCGTGGAGCCTACCAATTCCCGCAGAGCTTACCTCCAGGCAGGGTGCCATGAACACAGCACAGCAGG CATGTAAACCTCATCATCCAAATACTGAACCTGTATTAGGCCTCAGTCAAACACCAATTTCACAGCAATC CTTGCCACTACACATGATTCCTTCTAGCCAAGTAGATGACCTGTCCAGTCCTGCCAAGAGGAAAAGAACA TCTAGTCCAACAAAGAATACTTCTGACAATTGGAGTGGTGGACATGCTGTGTCACATCCTCCAGTACAGC AACAAGCTCATTCATGGTGTTTGACACCACAGAAATTACAGCATTTGGAACAGCTCCGCGCAAATAGAAA TAATTTAAATCCAGCACAGAAACTGATGCTGGAACAGCTGGAAAGTCAGTTTGTCTTAATGCAACAACAC CAAATGAGACCAACAGGAGTTGCACAGGTACGATCTACTGGAATTCCTAATGGGCCAACAGCTGACTCAT CACTGCCTACAAACTCAGTCTCTGGCCAGCAGCCACAGCTTGCTCTGACCAGAGTGCCTAGCGTCTCTCA GCCTGGAGTCCGTCCTGCCTGCCCTGGGCAGCCTTTGGCCAATGGACCCTTTTCTGCAGGCCATGTTCCC TGTAGCACATCAAGAACGCTGGGAAGTACAGACACTATTTTGATAGGCAATAATCATATAACAGGAAGTG GAAGTAATGGAAACGTGCCTTACCTGCAGCGAAACGCACTCACTCTACCTCATAACCGCACAAACCTGAC CAGCAGCGCAGAGGAGCCGTGGAAAAACCAACTATCTAACTCCACTCAGGGGCTTCACAAAGGTCAGAGT TCACATTCGGCAGGTCCTAATGGTGAACGACCTCTCTCTTCCACTGGGCCTTCCCAGCATCTCCAGGCAG CTGGCTCTGGTATTCAGAATCAGAACGGACATCCCACCCTGCCTAGCAATTCAGTAACACAGGGGGCTGC TCTCAATCACCTCTCCTCTCACACTGCTACCTCAGGTGGACAACAAGGCATTACCTTAACCAAAGAGAGC AAGCCTTCAGGAAACATATTGACGGTGCCTGAAACAAGCAGGCACACTGGAGAGACACCTAACAGCACTG CCAGTGTCGAGGGACTTCCTAATCATGTCCATCAGATGACGGCAGATGCTGTTTGCAGTCCTAGCCATGG AGATTCTAAGTCACCAGGTTTACTAAGTTCAGACAATCCTCAGCTCTCTGCCTTGTTGATGGGAAAAGCC AATAACAATGTGGGTACTGGAACCTGTGACAAAGTCAATAACATCCACCCAGCTGTTCATACAAAGACTG ATAACTCTGTTGCCTCTTCACCATCTTCAGCCATTTCAACAGCAACACCTTCTCCAAAATCCACTGAGCA GACAACCACAAACAGTGTTACCAGCCTTAACAGCCCTCACAGTGGGCTACACACAATTAATGGAGAAGGG ATGGAAGAATCTCAGAGCCCCATGAAAACAGATCTGCTTCTGGTTAACCACAAACCTAGTCCACAGATCA TACCATCAATGTCTGTGTCCATATACCCCAGCTCAGCAGAAGTTCTGAAGGCATGCAGGAATCTAGGTAA AAATGGCTTATCTAACAGTAGCATTTTGTTGGATAAATGTCCACCTCCAAGACCACCATCTTCACCATAC CCTCCCTTGCCAAAGGACAAGTTGAATCCACCTACACCTAGTATTTACTTGGAAAATAAACGTGATGCTT TCTTTCCTCCATTACATCAATTTTGTACAAATCCGAACAACCCTGTTACAGTAATACGTGGCCTTGCTGG AGCTCTTAAGTTAGACCTGGGACTTTTCTCTACTAAAACTTTGGTGGAAGCTAACAATGAACATATGGTA GAAGTGAGGACACAGTTGTTGCAGCCAGCAGATGAAAACTGGGATCCCACTGGAACAAAGAAAATCTGGC ATTGTGAAAGTAATAGATCTCATACTACAATTGCTAAATATGCACAGTACCAGGCCTCCTCATTCCAGGA ATCATTGAGAGAAGAAAATGAAAAAAGAAGTCATCATAAAGACCACTCAGATAGTGAATCTACATCGTCA GATAATTCTGGGAGGAGGAGGAAAGGACCCTTTAAAACCATAAAGTTTGGGACCAATATTGACCTATCTG ATGACAAAAAGTGGAAGTTGCAGCTACATGAGCTGACTAAACTTCCTGCTTTTGTGCGTGTCGTATCAGC AGGAAATCTTCTAAGCCATGTTGGTCATACCATATTGGGCATGAACACAGTTCAACTATACATGAAAGTT CCAGGGAGCAGAACACCAGGTCATCAGGAAAATAACAACTTCTGTTCAGTTAACATAAATATTGGCCCAG GTGACTGTGAATGGTTTGTTGTTCCTGAAGGTTACTGGGGTGTTCTGAATGACTTCTGTGAAAAAAATAA TTTGAATTTCCTAATGGGTTCTTGGTGGCCCAATCTTGAAGATCTTTATGAAGCAAATGTTCCAGTGTAT AGGTTTATTCAGCGACCTGGAGATTTGGTCTGGATAAATGCAGGCACTGTTCATTGGGTTCAGGCTATTG GCTGGTGCAACAACATTGCTTGGAATGTTGGTCCACTTACAGCCTGCCAGTATAAATTGGCAGTGGAACG GTACGAATGGAACAAATTGCAAAGTGTGAAGTCAATAGTACCCATGGTTCATCTTTCCTGGAATATGGCA CGAAATATCAAGGTCTCAGATCCAAAGCTTTTTGAAATGATTAAGTATTGTCTTCTAAGAACTCTGAAGC AATGTCAGACATTGAGGGAAGCTCTCATTGCTGCAGGAAAAGAGATTATATGGCATGGGCGGACAAAAGA AGAACCAGCTCATTACTGTAGCATTTGTGAAGTGGAGGTTTTTGATCTGCTTTTTGTCACTAATGAGAGT AATTCACGAAAGACCTACATAGTACATTGCCAAGATTGTGCACGAAAAACAAGCGGAAACTTGGAAAACT TTGTGGTGCTAGAACAGTACAAAATGGAGGACCTGATGCAAGTCTATGACCAATTTACATTAGCTCCTCC ATTACCATCCGCCTCATCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240152 representing NM_001291415 Red=Cloning site Green=Tags(s) MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTK ALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVY FHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFH IAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEAD PNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHG HAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIE EAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRT SSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQH QMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVP CSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQS SHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKES KPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKA NNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEG MEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPY PPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMV EVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSS DNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKV PGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVY RFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMA RNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNES NSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291415
ORF Size	4359 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291415.2
RefSeq Size	5941 bp
RefSeq ORF	4362 bp
Locus ID	7403
MW	160.2 kDa
Gene Summary	This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2014]

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