PITPNM2 (NM_001300801) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NIR-3; NIR3; RDGB2; RDGBA2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240117 representing NM_001300801 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTATAAAGGAATATCGGATTCCTCTGCCAATGACCGTGGAGGAGTACCGCATCGCCCAGCTGTACA TGATACAGAAGAAGAGCCGTAACGAGACATATGGCGAAGGCAGCGGCGTGGAGATCCTGGAGAACCGGCC GTACACAGATGGCCCAGGCGGCTCTGGGCAGTACACACACAAGGTGTATCATGTGGGCATGCACATTCCC AGCTGGTTCCGCTCCATCCTGCCCAAGGCAGCCCTGCGGGTGGTGGAGGAGTCTTGGAATGCCTACCCCT ACACCCGAACCAGGTTCACCTGTCCTTTCGTGGAGAAATTCTCCATCGACATTGAAACCTTTTATAAAAC TGATGCTGGAGAAAACCCCGACGTGTTCAACCTCTCTCCTGTGGAAAAGAACCAGCTGACAATCGACTTC ATCGACATTGTCAAAGACCCTGTGCCCCACAACGAGTATAAGACAGAAGAGGACCCCAAGCTGTTCCAGT CAACCAAGACCCAGCGGGGGCCCCTGTCCGAGAACTGGATCGAGGAGTACAAGAAGCAGGTCTTCCCCAT CATGTGCGCATACAAGCTCTGCAAGGTGGAGTTCCGCTACTGGGGCATGCAGTCCAAGATCGAGAGGTTC ATCCACGACACCGGACTACGGAGGGTGATGGTGCGGGCTCACCGGCAGGCCTGGTGCTGGCAGGACGAGT GGTATGGGCTGAGCATGGAGAACATCCGGGAGCTGGAGAAGGAGGCACAGCTCATGCTTTCCCGTAAGAT GGCCCAGTTCAATGAGGATGGTGAGGAGGCCACTGAGCTCGTCAAGCACGAAGCCGTCTCGGACCAGACC TCTGGGGAGCCCCCGGAGCCCAGCAGCAGCAATGGGGAGCCCCTAGTGGGGCGCGGCCTCAAGAAACAGT GGTCCACATCCTCCAAGTCGTCTCGGTCGTCCAAGCGGGGAGCGAGTCCTTCCCGCCACAGCATCTCAGA GTGGAGGATGCAGAGTATTGCCAGGGACTCGGATGAGAGCTCAGATGATGAGTTCTTCGATGCGCACGAG GACCTGTCCGACACAGAGGAAATGTTCCCCAAGGACATCACCAAGTGGAGCTCCAATGACCTCATGGACA AGATCGAGAGCCCAGAGCCGGAAGACACACAAGATGGTCTGTACCGCCAGGGTGCCCCTGAGTTCAGGGT GGCCTCCAGTGTGGAGCAGCTGAACATCATAGAGGACGAGGTTAGCCAGCCGCTGGCTGCACCGCCCTCC AAGATCCACGTGCTGCTACTGGTGCTGCACGGAGGCACCATCCTGGACACAGGCGCCGGGGACCCCAGCT CCAAGAAGGGCGATGCTAACACCATCGCCAACGTGTTCGACACCGTCATGCGCGTGCACTACCCCAGCGC CCTGGGCCGCCTTGCCATCCGCCTGGTGCCCTGCCCGCCCGTCTGCTCTGACGCCTTTGCCCTGGTCTCC AACCTCAGCCCCTACAGCCATGACGAAGGCTGTCTGTCCAGCAGTCAGGACCACATTCCCCTGGCTGCCC TCCCCCTGCTGGCCACCTCCTCCCCCCAGTACCAGGAGGCAGTTGCCACAGTGATTCAGCGAGCCAACCT TGCCTATGGGGACTTCATCAAGTCCCAGGAGGGCATGACCTTCAATGGGCAGGTCTGCCTGATTGGGGAC TGCGTCGGGGGCATCCTGGCATTTGATGCCCTGTGCTACAGTAACCAGCCGGTGTCTGAGAGTCAGAGCA GCAGCCGCCGGGGCAGCGTGGTCAGCATGCAGGACAATGACCTGCTGTCCCCGGGCATCCTGATGAATGC AGCACACTGCTGCGGTGGTGGCGGTGGCGGCGGTGGCGGTGGTGGCAGCAGTGGTGGTGGTGGCAGTAGT GGTGGCTCCAGCCTGGAGAGCAGTCGGCACCTGAGCCGAAGCAACGTCGACATCCCCCGCAGCAACGGCA CTGAGGACCCCAAAAGGCAACTGCCCCGCAAGAGGAGCGACTCATCCACCTACGAGCTGGATACCATCCA GCAGCACCAGGCCTTCCTGTCCAGCCTCCATGCCAGCGTGCTGAGGACTGAGCCCTGCTCACGCCATTCC AGCAGCTCCACCATGCTGGATGGCACAGGTGCCCTGGGCAGGTTTGACTTTGAGATCACCGACCTCTTCC TCTTCGGGTGCCCGCTGGGGCTGGTCCTGGCCTTGAGGAAGACTGTCATCCCAGCCCTGGATGTTTTCCA GCTGCGGCCGGCCTGCCAGCAAGTCTACAACCTCTTCCACCCCGCGGACCCGTCAGCTTCACGCCTGGAG CCGCTGCTGGAACGGCGCTTTCACGCCCTGCCGCCTTTCAGCGTCCCCCGCTACCAACGCTACCCGCTGG GGGATGGCTGCTCCACGCTGCTGGTCGAGACCGTGCAGAGAAACCCTGAGCTGGTCCTGGAGGGCGGCCC CCTGGCCCCTCTCCCCCACGGGGACGGCTTCCTGGAAACCAGTATGCCTGTTCCCGCGCCCACCTGGCAA GACGGGCCCCGCCCGGGCTGTGCCGAGTCGGATGTGCTCCAGACCCACAATGCAGCCTTCCAAGAGCATG GCGCCCCCTCCTCGCCGGGCACTGCCCCTGCCAGTCGTGGCTTCCGCCGAGCCAGTGAGATCAGCATCGC CAGCCAGGTGTCAGGCATGGCTGAGAGCTACACGGCATCCAGCATCGCCCAGATCGCTGCAAAGTGGTGG GGCCAGAAGCGGATCGACTACGCCCTGTACTGCCCTGACGCCCTCACGGCCTTCCCCACGGTGGCTCTGC CTCACCTCTTCCACGCCAGCTACTGGGAGTCAACAGACGTGGTCTCCTTTCTGCTGAGACAGGTCATGAG GCATGACAACTCCAGCATCTTGGAGCTGGATGGCAAGGAAGTGTCGGTGTTCACCCCCTCAAAGCCAAGG GAGAAGTGGCAGCGCAAGCGGACCCACGTGAAGCTGCGGAACGTGACGGCCAACCACCGGATCAATGATG CCCTTGCCAATGAGGACGGCCCCCAGGTTCTGACGGGCAGGTTCATGTATGGGCCCCTGGACATGGTCAC CCTGACTGGGGAGAAGGTGGATGTGCACATCATGACCCAGCCGCCCTCAGGCGAGTGGCTCTACCTGGAT ACGCTGGTGACCAACAACAGTGGGCGTGTCTCCTACACCATCCCTGAGTCGCACCGCCTGGGCGTGGGTG TCTACCCTATCAAGATGGTGGTCAGGGGAGACCACACGTTTGCCGACAGCTACATCACCGTGCTGCCCAA GGGCACAGAGTTCGTGGTCTTCAGCATCGACGGTTCCTTTGCCGCTAGCGTGTCCATCATGGGCAGCGAC CCCAAGGTGCGGGCCGGGGCCGTGGACGTGGTGCGGCACTGGCAGGACCTGGGCTACCTCATCATCTACG TGACGGGCCGGCCCGACATGCAGAAGCAGCGGGTGGTGGCGTGGCTGGCCCAGCACAACTTCCCCCATGG CGTGGTGTCCTTCTGTGACGGCCTGGTGCATGACCCGCTGCGGCACAAGGCCAACTTCCTGAAGCTGCTC ATCTCCGAGCTGCACCTGCGCGTGCACGCGGCCTATGGCTCCACCAAGGACGTGGCGGTGTACAGCGCCA TTAGCCTGTCCCCCATGCAGATCTACATCGTGGGCCGGCCCACCAAGAAGCTGCAGCAGCAGTGCCAGTT CATCACGGATGGCTACGCGGCCCACCTGGCGCAGCTGAAGTACAGCCACCGGGCGCGGCCCGCTCGCAAC ACGGCCACCCGCATGGCGCTGCGCAAGGGCAGCTTCGGCCTGCCCGGCCAGGGCGACTTTCTGCGCTCCC GGAACCACCTGCTTCGCACCATCTCGGCCCAGCCCAGCGGGCCCAGCCACCGGCACGAGCGGACACAGAG CCAGGCGGATGGCGAGCAGCGGGGCCAGCGCAGCATGAGTGTGGCGGCCGGCTGCTGGGGCCGCGCCATG ACTGGCCGCCTGGAGCCGGGGGCAGCCGCGGGCCCCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240117 representing NM_001300801 Red=Cloning site Green=Tags(s) MIIKEYRIPLPMTVEEYRIAQLYMIQKKSRNETYGEGSGVEILENRPYTDGPGGSGQYTHKVYHVGMHIP SWFRSILPKAALRVVEESWNAYPYTRTRFTCPFVEKFSIDIETFYKTDAGENPDVFNLSPVEKNQLTIDF IDIVKDPVPHNEYKTEEDPKLFQSTKTQRGPLSENWIEEYKKQVFPIMCAYKLCKVEFRYWGMQSKIERF IHDTGLRRVMVRAHRQAWCWQDEWYGLSMENIRELEKEAQLMLSRKMAQFNEDGEEATELVKHEAVSDQT SGEPPEPSSSNGEPLVGRGLKKQWSTSSKSSRSSKRGASPSRHSISEWRMQSIARDSDESSDDEFFDAHE DLSDTEEMFPKDITKWSSNDLMDKIESPEPEDTQDGLYRQGAPEFRVASSVEQLNIIEDEVSQPLAAPPS KIHVLLLVLHGGTILDTGAGDPSSKKGDANTIANVFDTVMRVHYPSALGRLAIRLVPCPPVCSDAFALVS NLSPYSHDEGCLSSSQDHIPLAALPLLATSSPQYQEAVATVIQRANLAYGDFIKSQEGMTFNGQVCLIGD CVGGILAFDALCYSNQPVSESQSSSRRGSVVSMQDNDLLSPGILMNAAHCCGGGGGGGGGGGSSGGGGSS GGSSLESSRHLSRSNVDIPRSNGTEDPKRQLPRKRSDSSTYELDTIQQHQAFLSSLHASVLRTEPCSRHS SSSTMLDGTGALGRFDFEITDLFLFGCPLGLVLALRKTVIPALDVFQLRPACQQVYNLFHPADPSASRLE PLLERRFHALPPFSVPRYQRYPLGDGCSTLLVETVQRNPELVLEGGPLAPLPHGDGFLETSMPVPAPTWQ DGPRPGCAESDVLQTHNAAFQEHGAPSSPGTAPASRGFRRASEISIASQVSGMAESYTASSIAQIAAKWW GQKRIDYALYCPDALTAFPTVALPHLFHASYWESTDVVSFLLRQVMRHDNSSILELDGKEVSVFTPSKPR EKWQRKRTHVKLRNVTANHRINDALANEDGPQVLTGRFMYGPLDMVTLTGEKVDVHIMTQPPSGEWLYLD TLVTNNSGRVSYTIPESHRLGVGVYPIKMVVRGDHTFADSYITVLPKGTEFVVFSIDGSFAASVSIMGSD PKVRAGAVDVVRHWQDLGYLIIYVTGRPDMQKQRVVAWLAQHNFPHGVVSFCDGLVHDPLRHKANFLKLL ISELHLRVHAAYGSTKDVAVYSAISLSPMQIYIVGRPTKKLQQQCQFITDGYAAHLAQLKYSHRARPARN TATRMALRKGSFGLPGQGDFLRSRNHLLRTISAQPSGPSHRHERTQSQADGEQRGQRSMSVAAGCWGRAM TGRLEPGAAAGPK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001300801
ORF Size	4029 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300801.1, NP_001287730.1
RefSeq Size	6797 bp
RefSeq ORF	4032 bp
Locus ID	57605
UniProt ID	Q9BZ72
Protein Families	Druggable Genome
MW	148.9 kDa
Gene Summary	PITPNM2 belongs to a family of membrane-associated phosphatidylinositol transfer domain-containing proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein (Ocaka et al., 2005 [PubMed 15627748]).[supplied by OMIM, Mar 2008]

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