MCM8 (NM_001281521) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C20orf154; dJ967N21.5; POF10
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239725 representing NM_001281521 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGAGAGTATAGAGGCAGAGGATTTGGACGAGGAAGATTTCAAAGCTGGAAAAGGGGAAGAGGTG GTGGGAACTTCTCAGGAAAATGGAGAGAAAGAGAACACAGACCTGATCTGAGTAAAACCACAGGAAAACG TACTTCTGAACAAACCCCACAGTTTTTGCTTTCAACAAAGACCCCACAGTCAATGCAGTCAACATTGGAT CGATTCATACCATATAAAGGCTGGAAGCTTTATTTCTCTGAAGTTTACAGCGATAGCTCTCCTTTGATTG AGAAGATTCAAGCATTTGAAAAATTTTTCACAAGGCATATTGATTTGTATGACAAGGATGAAATAGAAAG AAAGGGAAGTATTTTGGTAGATTTTAAAGAACTGACAGAAGGTGGTGAAGTAACTAACTTGATACCAGAT ATAGCAACTGAACTAAGAGATGCACCTGAGAAAACCTTGGCTTGCATGGGTTTGGCAATACATCAGGTGT TAACTAAGGACCTTGAAAGGCATGCAGCTGAGTTACAAGCCCAGGAAGGATTGTCTAATGATGGAGAAAC AATGGTAAATGTGCCACATATTCATGCAAGGGTGTACAACTATGAGCCTTTGACACAGCTCAAGAATGTC AGAGCAAATTACTATGGAAAATACATTGCTCTAAGAGGGACAGTGGTTCGTGTCAGTAATATAAAGCCTC TTTGCACCAAGATGGCTTTTCTTTGTGCTGCATGTGGAGAAATTCAGAGCTTTCCTCTTCCAGATGGAAA ATACAGTCTTCCCACAAAGTGTCCTGTGCCTGTGTGTCGAGGCAGGTCATTTACTGCTCTCCGCAGCTCT CCTCTCACAGTTACGATGGACTGGCAGTCAATCAAAATCCAGGAATTGATGTCTGATGATCAGAGAGAAG CAGGTCGGATTCCACGAACAATAGAATGTGAGCTTGTTCATGATCTTGTGGATAGCTGTGTCCCGGGAGA CACAGTGACTATTACTGGAATTGTCAAAGTCTCAAATGCGGAAGAAGGTTCTCGAAATAAGAATGACAAG TGTATGTTCCTTTTGTATATTGAAGCAAATTCTATTAGTAATAGCAAAGGACAGAAAACAAAGAGTTCTG AGGATGGGTGTAAGCATGGAATGTTGATGGAGTTCTCACTTAAAGACCTTTATGCCATCCAAGAGATTCA AGCTGAAGAAAACCTGTTTAAACTCATTGTCAAATGGAGTCTTGCTCTGTCGCCCAGGCTAGAGTACAGT GGTGCGATCTCAGCTCACTGCAACCTCCACCTCCCAAGTTCAAACAGTTCTCCCACCTCAGCCTGCCGAG TAGCTGGGACTACAGGCATGCGCCACCAGACCCAGCTACTTTTGCTTGTTAAAGCAGGTTTGGCATTAGC ACTCTTTGGAGGAAGCCAGAAATACGCAGATGACAAAAACAGAATTCCAATTCGGGGAGACCCCCACATC CTTGTTGTTGGAGATCCAGGCCTAGGAAAAAGTCAAATGCTACAGGCAGCGTGCAATGTTGCCCCACGTG GCGTGTATGTTTGTGGTAACACCACGACCACCTCTGGTCTGACGGTAACTCTTTCAAAAGATAGTTCCTC TGGAGATTTTGCTTTGGAAGCTGGTGCCCTGGTACTTGGTGATCAAGGTATTTGTGGAATCGATGAATTT GATAAGATGGGGAATCAACATCAAGCCTTGTTGGAAGCCATGGAGCAGCAAAGTATTAGTCTTGCTAAGG CTGGTGTGGTTTGTAGCCTTCCTGCAAGAACTTCCATTATTGCTGCTGCAAATCCAGTTGGAGGACATTA CAATAAAGCCAAAACAGTTTCTGAGAATTTAAAAATGGGGAGTGCACTACTATCCAGATTTGATTTGGTC TTTATCCTGTTAGATACTCCAAATGAGCATCATGATCACTTACTCTCTGAACATGTGATTGCAATAAGAG CTGGAAAGCAGAGAACCATTAGCAGTGCCACAGTAGCTCGTATGAATAGTCAAGATTCAAATACTTCCGT ACTTGAAGTAGTTTCTGAGAAGCCATTATCAGAAAGACTAAAGGTGGTTCCTGGAGAAACAATAGATCCC ATTCCCCACCAGCTATTGAGAAAGTACATTGGCTATGCTCGGCAGTATGTGTACCCAAGGCTATCCACAG AAGCTGCTCGAGTTCTTCAAGATTTTTACCTTGAGCTCCGGAAACAGAGCCAGAGGTTAAATAGCTCACC AATCACTACCAGGCAGCTGGAATCTTTGATTCGTCTGACAGAGGCACGAGCAAGGTTGGAATTGAGAGAG GAAGCAACCAAAGAAGACGCTGAGGATATAGTGGAAATTATGAAATATAGCATGCTAGGAACTTACTCTG ATGAATTTGGGAACCTAGATTTTGAGCGATCCCAGCATGGTTCTGGAATGAGCAACAGGTCAACAGCGAA AAGATTTATTTCTGCTCTCAACAACGTTGCTGAAAGAACTTATAATAATATATTTCAATTTCATCAACTT CGGCAGATTGCCAAAGAACTAAACATTCAGGTTGCTGATTTTGAAAATTTTATTGGATCACTAAATGACC AGGGTTACCTCTTGAAAAAAGGCCCAAAAGTTTACCAGCTTCAAACTATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239725 representing NM_001281521 Red=Cloning site Green=Tags(s) MNGEYRGRGFGRGRFQSWKRGRGGGNFSGKWREREHRPDLSKTTGKRTSEQTPQFLLSTKTPQSMQSTLD RFIPYKGWKLYFSEVYSDSSPLIEKIQAFEKFFTRHIDLYDKDEIERKGSILVDFKELTEGGEVTNLIPD IATELRDAPEKTLACMGLAIHQVLTKDLERHAAELQAQEGLSNDGETMVNVPHIHARVYNYEPLTQLKNV RANYYGKYIALRGTVVRVSNIKPLCTKMAFLCAACGEIQSFPLPDGKYSLPTKCPVPVCRGRSFTALRSS PLTVTMDWQSIKIQELMSDDQREAGRIPRTIECELVHDLVDSCVPGDTVTITGIVKVSNAEEGSRNKNDK CMFLLYIEANSISNSKGQKTKSSEDGCKHGMLMEFSLKDLYAIQEIQAEENLFKLIVKWSLALSPRLEYS GAISAHCNLHLPSSNSSPTSACRVAGTTGMRHQTQLLLLVKAGLALALFGGSQKYADDKNRIPIRGDPHI LVVGDPGLGKSQMLQAACNVAPRGVYVCGNTTTTSGLTVTLSKDSSSGDFALEAGALVLGDQGICGIDEF DKMGNQHQALLEAMEQQSISLAKAGVVCSLPARTSIIAAANPVGGHYNKAKTVSENLKMGSALLSRFDLV FILLDTPNEHHDHLLSEHVIAIRAGKQRTISSATVARMNSQDSNTSVLEVVSEKPLSERLKVVPGETIDP IPHQLLRKYIGYARQYVYPRLSTEAARVLQDFYLELRKQSQRLNSSPITTRQLESLIRLTEARARLELRE EATKEDAEDIVEIMKYSMLGTYSDEFGNLDFERSQHGSGMSNRSTAKRFISALNNVAERTYNNIFQFHQL RQIAKELNIQVADFENFIGSLNDQGYLLKKGPKVYQLQTM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001281521
ORF Size	2640 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281521.1, NP_001268450.1
RefSeq Size	3835 bp
RefSeq ORF	2643 bp
Locus ID	84515
UniProt ID	Q9UJA3
Protein Families	Transcription Factors
MW	98.4 kDa
Gene Summary	The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the mini-chromosome maintenance proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the mini-chromosome maintenance proteins. The encoded protein may interact with other mini-chromosome maintenance proteins and play a role in DNA replication. This gene may be associated with length of reproductive lifespan and menopause. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2013]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.