CDHR3 (NM_001301161) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CDH28
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239588 representing NM_001301161 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACAACTAGATTTTGAAACAGGACCAAACATATTTGATTTGCAGATTTATGTGAAGGATGAGGTTG GTGTCACAGACCTGCAAGTCCTGACTGTCCAGGTAACAGATGTGAACGAGCCACCTCAGTTTCAAGGCAA CTTGGCAGAAGGTCTACACCTCTACATAGTAGAAAGAGCAAACCCTGGATTCATTTACCAGGTTGAGGCC TTCGATCCAGAAGACACAAGCCGAAACATTCCCCTCAGTTATTTCCTGATTTCTCCCCCAAAGAGCTTCA GAATGTCTGCTAATGGCACCCTCTTCTCCACAACAGAATTGGACTTTGAAGCAGGACACAGAAGTTTCCA TCTCATCGTGGAGGTGAGGGACAGTGGAGGCCTCAAAGCCTCCACAGAGCTCCAGGTGAACATCGTGAAC CTCAACGACGAAGTCCCTCGCTTTACCAGCCCGACACGAGTGTACACAGTCCTGGAGGAACTGAGTCCAG GAACCATCGTGGCCAATATCACAGCGGAGGATCCTGATGATGAAGGTTTTCCCAGCCACCTCCTCTACAG CATTACCACTGTTAGCAAATATTTCATGATAAATCAGTTGACTGGTACAATCCAAGTGGCCCAAAGGATA GACCGAGATGCAGGTGAATTGAGACAAAATCCCACCATTTCCCTGGAAGTTCTAGTGAAGGACAGACCAT ATGGGGGTCAGGAGAATCGCATCCAGATAACCTTCATTGTGGAAGACGTCAACGACAATCCTGCCACATG CCAAAAGTTCACCTTCAGCATTATGGTGCCGGAAAGAACAGCCAAGGGGACGTTGCTTCTTGACCTAAAC AAGTTCTGCTTTGATGATGACAGTGAGGCACCAAACAACAGATTCAACTTCACCATGCCATCTGGAGTGG GGAGCGGCAGCAGATTTTTACAGGATCCAGCTGGCTCTGGGAAGATTGTGCTGATTGGTGATCTAGACTA CGAAAATCCAAGTAACCTAGCAGCCGGCAATAAATATACGGTGATAATCCAGGTGCAGGATGTGGCCCCC CCTTACTATAAAAATAACGTCTACGTTTATATCCTAACAAGCCCAGAAAATGAGTTTCCTCTCATTTTTG ATAGGCCATCCTATGTATTTGATGTGTCAGAAAGAAGGCCCGCCAGAACCCGAGTGGGACAGGTGCGAGC CACTGATAAAGACCTCCCCCAGAGCAGCCTCCTGTACTCCATCTCCACTGGAGGGGCCAGCCTCCAGTAT CCAAATGTATTTTGGATTAATCCCAAGACAGGAGAACTCCAGCTGGTAACTAAAGTGGACTGTGAAACAA CCCCCATCTATATTCTCAGAATCCAGGCCACCAACAACGAAGACACAAGCTCTGTCACTGTTACTGTGAA CATCCTTGAAGAAAATGATGAAAAGCCAATTTGTACTCCAAACTCTTATTTCCTGGCCCTCCCAGTGGAT CTGAAAGTTGGCACAAATATTCAGAATTTCAAGCTGACATGTACCGACCTTGATTCCAGCCCCAGATCTT TCCGTTATTCCATTGGCCCAGGTAACGTCAACAATCATTTCACCTTCTCTCCCAATGCTGGTTCCAATGT CACACGCCTGCTGCTTACATCTCGCTTTGACTATGCTGGTGGGTTTGATAAGATCTGGGACTACAAGCTA CTTGTCTACGTAACTGATGACAACTTGATGTCTGACAGGAAGAAAGCGGAGGCTCTTGTTGAGACAGGAA CAGTGACACTGAGTATTAAAGTCATTCCCCACCCAACCACTATCATCACCACGACCCCCAGGCCCAGGGT CACCTATCAGGTCCTGAGGAAAAACGTTTACTCTCCATCTGCATGGTACGTGCCGTTTGTCATCACTTTG GGCTCCATATTGCTTCTGGGTCTCCTCGTGTACCTGGTCGTCCTATTGGCCAAAGCCATCCACAGACACT GCCCCTGCAAGACTGGGAAGAACAAGGAACCTCTGACAAAGAAAGGAGAAACGAAGACTGCAGAGAGAGA CGTCGTGGTGGAAACTATCCAGATGAACACTATCTTTGATGGAGAAGCCATAGATCCAGTGACCGGGGAA ACATATGAATTCAACTCAAAAACTGGAGCCAGAAAGTGGAAAGATCCACTAACCCAAATGCCAAAATGGA AAGAGTCCAGCCACCAGGGAGCTGCCCCACGCAGAGTCACTGCTGGGGAAGGGATGGGGTCACTGAGAAG TGCCAACTGGGAAGAAGATGAGCTGAGTGGCAAAGCGTGGGCTGAGGATGCTGGTCTGGGTTCCAGAAAT GAGGGTGGCAAGCTGGGCAACCCAAAGAACAGAAATCCAGCCTTCATGAACAGGGCTTACCCCAAACCAC ACCCAGGAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239588 representing NM_001301161 Red=Cloning site Green=Tags(s) MEQLDFETGPNIFDLQIYVKDEVGVTDLQVLTVQVTDVNEPPQFQGNLAEGLHLYIVERANPGFIYQVEA FDPEDTSRNIPLSYFLISPPKSFRMSANGTLFSTTELDFEAGHRSFHLIVEVRDSGGLKASTELQVNIVN LNDEVPRFTSPTRVYTVLEELSPGTIVANITAEDPDDEGFPSHLLYSITTVSKYFMINQLTGTIQVAQRI DRDAGELRQNPTISLEVLVKDRPYGGQENRIQITFIVEDVNDNPATCQKFTFSIMVPERTAKGTLLLDLN KFCFDDDSEAPNNRFNFTMPSGVGSGSRFLQDPAGSGKIVLIGDLDYENPSNLAAGNKYTVIIQVQDVAP PYYKNNVYVYILTSPENEFPLIFDRPSYVFDVSERRPARTRVGQVRATDKDLPQSSLLYSISTGGASLQY PNVFWINPKTGELQLVTKVDCETTPIYILRIQATNNEDTSSVTVTVNILEENDEKPICTPNSYFLALPVD LKVGTNIQNFKLTCTDLDSSPRSFRYSIGPGNVNNHFTFSPNAGSNVTRLLLTSRFDYAGGFDKIWDYKL LVYVTDDNLMSDRKKAEALVETGTVTLSIKVIPHPTTIITTTPRPRVTYQVLRKNVYSPSAWYVPFVITL GSILLLGLLVYLVVLLAKAIHRHCPCKTGKNKEPLTKKGETKTAERDVVVETIQMNTIFDGEAIDPVTGE TYEFNSKTGARKWKDPLTQMPKWKESSHQGAAPRRVTAGEGMGSLRSANWEEDELSGKAWAEDAGLGSRN EGGKLGNPKNRNPAFMNRAYPKPHPGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001301161
ORF Size	2391 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301161.2
RefSeq Size	6297 bp
RefSeq ORF	2394 bp
Locus ID	222256
UniProt ID	Q6ZTQ4
Protein Families	Transmembrane
MW	89.3 kDa
Gene Summary	Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.[UniProtKB/Swiss-Prot Function]

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