MTSS1 (NM_001282971) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MIM; MIMA; MIMB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239504 representing NM_001282971 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCTGTGATTGAGAAGGAATGCAGCGCGCTCGGAGGCCTCTTCCAGACCATCATCAGCGACATGA AGGGGAGCTATCCAGTTTGGGAAGATTTCATAAACAAAGCAGGAAAGCTGCAGTCCCAGCTTCGGACAAC AGTAGTAGCAGCAGCTGCCTTCTTGGACGCCTTTCAGAAAGTGGCTGACATGGCCACCAACACACGTGGT GGGACCAGGGAGATTGGATCTGCTCTCACCAGGATGTGCATGAGGCACAGAAGCATTGAAGCCAAGCTGA GGCAGTTTTCGAGCGCTTTAATTGATTGTCTGATAAACCCACTTCAAGAACAGATGGAAGAATGGAAGAA AGTGGCCAACCAGCTGGATAAAGACCACGCAAAAGAATATAAGAAAGCCCGCCAAGAGATAAAAAAGAAG TCCTCGGATACGCTGAAACTGCAGAAGAAAGCAAAAAAAGTGGACACTCTTGGGAGAGGTGATATCCAGC CTCAGTTGGACAGTGCTCTCCAAGATGTCAATGATAAGTATCTCTTATTGGAAGAAACAGAAAAGCAGGC TGTCCGGAAGGCTTTGATTGAAGAACGTGGCCGATTCTGTACCTTCATCTCTATGCTGCGGCCAGTGATT GAAGAAGAAATCTCAATGCTAGGGGAAATAACCCACCTTCAGACCATCTCGGAAGATCTAAAAAGCCTGA CCATGGACCCTCACAAACTGCCCTCCTCAAGTGAACAGGTGATTCTGGACTTGAAAGGTTCTGATTACAG CTGGTCGTATCAGACGCCACCCTCTTCCCCCAGCACCACCATGTCCAGAAAGTCCAGTGTCTGCAGCAGC CTGAACAGTGTCAACAGCAGTGACTCCCGGTCCAGCGGCTCCCACTCGCATTCCCCCAGCTCACATTACC GCTACCGCAGCTCCAACCTGGCCCAGCAGGCTCCTGTGAGGCTGTCCAGCGTGTCCTCCCATGACTCAGG ATTCATATCCCAGGATGCCTTCCAGTCCAAGTCACCATCCCCCATGCCGCCAGAGGCCCCCAACCAGTTG TCTAACGGGTTTTCTCACTATAGTTTATCAAGTGAGTCCCACGTGGGGCCCACGGGTGCAGGCCTTTTCC CTCATTGCCTGCCTGCCTCCCGCCTGCTCCCTCGGGTCACCTCTGTCCACCTTCCAGACTACGCTCATTA TTACACCATTGGGCCCGGCATGTTCCCGTCATCTCAGATCCCTAGCTGGAAGGACTGGGCTAAGCCTGGG CCCTATGACCAGCCTCTGGTGAACACCCTGCAGCGCCGCAAAGAGAAGCGAGAACCGGACCCCAACGGGG GAGGACCCACTACCGCCAGCGGCCCACCTGCAGCAGCTGAGGAGGCTCAGAGACCACGGAGCATGACTGT ATCGGCTGCCACCAGGCCTGGTGAGGAGATGGAGGCTTGTGAGGAGCTGGCCCTGGCCCTGTCTCGGGGC CTGCAGCTGGACACCCAGAGGAGCAGCCGGGACTCGCTTCAGTGCTCCAGCGGCTACAGCACCCAGACAA CCACCCCCTGCTGCTCTGAGGACACCATCCCTTCCCAAGTTTCAGATTATGATTATTTCTCTGTAAGTGG TGACCAGGAGGCAGATCAGCAGGAGTTCGACAAGTCCTCCACCATTCCAAGAAACAGCGACATCAGCCAG TCCTACCGACGGATGTTCCAAGCCAAGCGTCCAGCCTCAACTGCTGGCCTCCCCACCACCCTGGGACCTG CTATGGTCACTCCAGGGGTTGCAACTATCCGACGGACCCCTTCCACCAAGCCTTCTGTCCGCCGGGGAAC CATTGGAGCTGGTCCCATCCCCATCAAGACACCCGTGATCCCTGTCAAGACCCCAACCGTCCCAGACCTC CCAGGGGTGTTGCCAGCCCCTCCAGATGGGCCAGAAGAGCGGGGGGAGCACAGCCCTGAGTCGCCATCTG TGGGTGAGGGCCCCCAAGGTGTCACCAGCATGCCCTCCTCAATGTGGAGCGGCCAAGCTTCCGTTAACCC TCCACTTCCAGGCCCGAAGCCCAGTATCCCTGAGGAGCACAGACAGGCAATTCCAGAAAGTGAAGCTGAA GACCAGGAACGGGAACCCCCAAGTGCCACTGTCTCCCCAGGCCAGATTCCAGAGAGTGACCCTGCAGACC TGAGCCCAAGGGATACTCCACAAGGAGAAGACATGCTGAACGCCATCCGAAGGGGCGTGAAACTGAAGAA GACCACGACAAACGATCGCTCAGCCCCTCGCTTTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239504 representing NM_001282971 Red=Cloning site Green=Tags(s) MEAVIEKECSALGGLFQTIISDMKGSYPVWEDFINKAGKLQSQLRTTVVAAAAFLDAFQKVADMATNTRG GTREIGSALTRMCMRHRSIEAKLRQFSSALIDCLINPLQEQMEEWKKVANQLDKDHAKEYKKARQEIKKK SSDTLKLQKKAKKVDTLGRGDIQPQLDSALQDVNDKYLLLEETEKQAVRKALIEERGRFCTFISMLRPVI EEEISMLGEITHLQTISEDLKSLTMDPHKLPSSSEQVILDLKGSDYSWSYQTPPSSPSTTMSRKSSVCSS LNSVNSSDSRSSGSHSHSPSSHYRYRSSNLAQQAPVRLSSVSSHDSGFISQDAFQSKSPSPMPPEAPNQL SNGFSHYSLSSESHVGPTGAGLFPHCLPASRLLPRVTSVHLPDYAHYYTIGPGMFPSSQIPSWKDWAKPG PYDQPLVNTLQRRKEKREPDPNGGGPTTASGPPAAAEEAQRPRSMTVSAATRPGEEMEACEELALALSRG LQLDTQRSSRDSLQCSSGYSTQTTTPCCSEDTIPSQVSDYDYFSVSGDQEADQQEFDKSSTIPRNSDISQ SYRRMFQAKRPASTAGLPTTLGPAMVTPGVATIRRTPSTKPSVRRGTIGAGPIPIKTPVIPVKTPTVPDL PGVLPAPPDGPEERGEHSPESPSVGEGPQGVTSMPSSMWSGQASVNPPLPGPKPSIPEEHRQAIPESEAE DQEREPPSATVSPGQIPESDPADLSPRDTPQGEDMLNAIRRGVKLKKTTTNDRSAPRFS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282971
ORF Size	2277 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282971.1, NP_001269900.1
RefSeq Size	5066 bp
RefSeq ORF	2280 bp
Locus ID	9788
UniProt ID	O43312
Protein Families	Druggable Genome
MW	83.1 kDa
Gene Summary	May be related to cancer progression or tumor metastasis in a variety of organ sites, most likely through an interaction with the actin cytoskeleton.[UniProtKB/Swiss-Prot Function]

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