WDR48 (NM_001303403) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Bun62; P80; SPG60; UAF1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239272 representing NM_001303403 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCCATCACCGGCAGAACACAGCAGGGCGGAGGAAAGTGCAGGTTTCCTATGTTATTCGAGATG AAGTGGAGAAGTACAACCGAAATGGAGTCAATGCTCTGCAGCTGGATCCAGCACTAAATAGACTTTTCAC AGCCGGTCGAGACTCTATCATAAGAATATGGAGTGTCAATCAGCACAAGCAAGATCCATATATAGCATCT ATGGAACACCATACTGATTGGGTAAACGACATTGTACTCTGTTGTAATGGGAAAACATTAAAAGTATGGA ATGCACACAAGGGATTTTGCATGTCAACATTAAGGACACATAAGGATTACGTAAAGGCCTTAGCATATGC CAAGGATAAAGAACTAGTAGCATCAGCTGGGTTGGACAGACAAATATTCCTTTGGGATGTGAATACTCTA ACAGCATTGACTGCCTCAAATAACACTGTCACAACTTCTTCTTTAAGTGGAAACAAAGATTCCATTTATA GCCTGGCCATGAATCAACTGGGAACAATCATTGTATCAGGGTCCACTGAAAAGGTGTTACGGGTATGGGA TCCAAGAACATGTGCAAAACTAATGAAGCTTAAAGGGCACACGGATAATGTGAAGGCATTGCTATTAAAC AGAGATGGCACGCAATGCCTGTCAGGCAGTTCTGATGGGACAATTCGCCTTTGGTCCCTTGGCCAGCAGA GATGTATAGCAACATACCGAGTCCATGATGAAGGTGTTTGGGCGCTGCAAGTCAATGATGCCTTCACACA TGTGTATTCTGGTGGAAGGGACAGGAAGATTTATTGTACAGACCTAAGAAACCCTGACATTCGGGTGCTA ATTTGTGAAGAAAAAGCACCAGTTCTCAAGATGGAGCTTGATAGATCAGCTGATCCTCCTCCTGCAATTT GGGTTGCAACAACTAAGTCTACAGTAAATAAATGGACTTTGAAAGGAATTCATAATTTTAGAGCCTCTGG AGATTATGACAATGACTGTACAAATCCTATAACACCTCTTTGTACACAACCTGACCAGGTTATTAAAGGG GGTGCTAGTATTATTCAGTGCCACATTCTTAATGATAAGAGACATATATTAACCAAAGATACCAATAATA ATGTGGCATATTGGGATGTATTGAAGGCATGTAAAGTTGAAGATCTGGGCAAAGTGGATTTTGAAGATGA AATTAAGAAAAGATTTAAAATGGTGTATGTGCCAAATTGGTTCTCAGTAGACTTAAAAACAGGGATGTTA ACTATTACTTTGGATGAAAGTGATTGTTTTGCTGCCTGGGTTTCTGCAAAAGATGCTGGTTTCAGCAGCC CTGATGGGTCAGATCCAAAATTGAATTTAGGAGGACTTTTACTCCAAGCACTCCTGGAATATTGGCCTAG AACACATGTGAATCCAATGGATGAAGAGGAAAATGAAGTAAACCATGTAAATGGGGAGCAGGAGAACCGA GTGCAGAAGGGAAATGGATATTTTCAAGTGCCCCCACATACACCCGTGATCTTTGGTGAAGCTGGAGGTC GCACACTGTTCAGGCTGCTCTGCCGAGATTCCGGGGGTGAGACTGAGTCTATGCTTCTTAATGAAACAGT GCCACAATGGGTAATTGACATCACTGTGGATAAAAATATGCCCAAATTCAACAAAATTCCTTTCTACCTC CAACCTCATGCATCTTCAGGAGCAAAAACCTTAAAAAAAGATAGACTCTCTGCTAGTGACATGCTCCAAG TCCGAAAAGTTATGGAACATGTTTATGAAAAAATTATCAACTTGGATAATGAGTCTCAAACCACTAGCTC TTCTAATAATGAAAAACCAGGAGAACAGGAAAAAGAAGAAGATATTGCTGTGTTGGCAGAGGAGAAAATT GAACTTTTGTGCCAGGACCAGGTTTTGGATCCAAATATGGACCTTCGAACAGTGAAACACTTCATATGGA AGAGCGGTGGAGACCTCACCCTCCATTACCGTCAGAAGTCCACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239272 representing NM_001303403 Red=Cloning site Green=Tags(s) MAAHHRQNTAGRRKVQVSYVIRDEVEKYNRNGVNALQLDPALNRLFTAGRDSIIRIWSVNQHKQDPYIAS MEHHTDWVNDIVLCCNGKTLKVWNAHKGFCMSTLRTHKDYVKALAYAKDKELVASAGLDRQIFLWDVNTL TALTASNNTVTTSSLSGNKDSIYSLAMNQLGTIIVSGSTEKVLRVWDPRTCAKLMKLKGHTDNVKALLLN RDGTQCLSGSSDGTIRLWSLGQQRCIATYRVHDEGVWALQVNDAFTHVYSGGRDRKIYCTDLRNPDIRVL ICEEKAPVLKMELDRSADPPPAIWVATTKSTVNKWTLKGIHNFRASGDYDNDCTNPITPLCTQPDQVIKG GASIIQCHILNDKRHILTKDTNNNVAYWDVLKACKVEDLGKVDFEDEIKKRFKMVYVPNWFSVDLKTGML TITLDESDCFAAWVSAKDAGFSSPDGSDPKLNLGGLLLQALLEYWPRTHVNPMDEEENEVNHVNGEQENR VQKGNGYFQVPPHTPVIFGEAGGRTLFRLLCRDSGGETESMLLNETVPQWVIDITVDKNMPKFNKIPFYL QPHASSGAKTLKKDRLSASDMLQVRKVMEHVYEKIINLDNESQTTSSSNNEKPGEQEKEEDIAVLAEEKI ELLCQDQVLDPNMDLRTVKHFIWKSGGDLTLHYRQKST TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001303403
ORF Size	2004 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001303403.2
RefSeq Size	4000 bp
RefSeq ORF	2007 bp
Locus ID	57599
UniProt ID	Q8TAF3
MW	75.8 kDa
Gene Summary	The protein encoded by this gene has been shown to interact with ubiquitin specific peptidase 1 (USP1), activating the deubiquitinating activity of USP1 and allowing it to remove the ubiquitin moiety from monoubiquitinated FANCD2. FANCD2 is ubiquitinated in response to DNA damage. [provided by RefSeq, Sep 2016]

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