Rho GTPase activating protein 24 (ARHGAP24) (NM_001287805) Human Tagged ORF Clone
CAT#: RC239254
- TrueORF®
ARHGAP24 (myc-DDK-tagged) - Human Rho GTPase activating protein 24 (ARHGAP24), transcript variant 4
ORF Plasmid: tGFP
"NM_001287805" in other vectors (2)
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CNY 4,560.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | FILGAP; p73; p73RhoGAP; RC-GAP72; RCGAP72 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239254 representing NM_001287805
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGTGCAAGGAGGCGATCGAGATCGGATGACAGCAAATCATGAAAGCTACCTCCTCATGGCAAGCA CCCAGAATGATATGGAAGACTGGGTGAAGTCAATCCGCCGAGTCATATGGGGACCTTTCGGAGGAGGCAT TTTTGGACAGAAACTGGAGGATACTGTTCGTTATGAGAAGAGATATGGGAACCGTCTGGCTCCGATGTTG GTGGAGCAGTGCGTGGACTTTATCCGACAAAGGGGGCTGAAAGAAGAGGGTCTCTTTCGACTGCCAGGCC AGGCTAATCTTGTTAAGGAGCTCCAAGATGCCTTTGACTGTGGGGAGAAGCCATCATTTGACAGCAACAC AGATGTACACACGGTGGCATCACTTCTTAAGCTGTACCTCCGAGAACTTCCAGAACCAGTTATTCCTTAT GCGAAGTATGAAGATTTTTTGTCATGTGCCAAACTGCTCAGCAAGGAAGAGGAAGCAGGTGTTAAGGAAT TAGCAAAGCAGGTGAAGAGTTTGCCAGTGGTAAATTACAACCTCCTCAAGTATATTTGCAGATTCTTGGA TGAAGTACAGTCCTACTCGGGAGTTAACAAAATGAGTGTGCAGAACTTGGCAACGGTCTTTGGTCCTAAT ATCCTGCGCCCCAAAGTGGAAGATCCTTTGACTATCATGGAGGGCACTGTGGTGGTCCAGCAGTTGATGT CAGTGATGATTAGCAAACATGATTGCCTCTTTCCCAAAGATGCAGAACTACAAAGCAAGCCCCAAGATGG AGTGAGCAACAACAATGAAATTCAGAAGAAAGCCACCATGGGGCAGTTACAGAACAAGGAGAACAATAAC ACCAAGGACAGCCCTAGTAGGCAGTGCTCCTGGGACAAGTCTGAGTCACCCCAGAGAAGCAGCATGAACA ATGGATCCCCCACAGCTCTATCAGGCAGCAAAACCAACAGCCCAAAGAACAGTGTTCACAAGCTAGATGT GTCTAGAAGCCCCCCTCTCATGGTCAAAAAGAACCCAGCCTTTAATAAGGGTAGTGGGATAGTTACCAAT GGGTCCTTCAGCAGCAGTAATGCAGAAGGTCTTGAGAAAACCCAAACCACCCCCAATGGGAGCCTACAGG CCAGAAGGAGCTCTTCACTGAAGGTATCTGGTACCAAAATGGGCACGCACAGTGTACAGAATGGAACGGT GCGCATGGGCATTTTGAACAGCGACACACTCGGGAACCCCACAAATGTTCGAAACATGAGCTGGCTGCCA AATGGCTATGTGACCCTGAGGGATAACAAGCAGAAAGAACAAGCTGGAGAGTTAGGCCAGCACAACAGAC TGTCCACCTATGATAATGTCCATCAACAGTTCTCCATGATGAACCTTGATGACAAGCAGAGCATTGACAG TGCTACCTGGTCCACTTCCTCCTGTGAAATCTCCCTCCCTGAGAACTCCAACTCCTGTCGCTCTTCTACC ACCACCTGCCCAGAGCAAGACTTTTTTGGGGGGAACTTTGAGGACCCTGTTTTGGATGGGCCCCCGCAGG ACGACCTTTCCCACCCCAGGGACTATGAAAGCAAAAGTGACCACAGGAGTGTGGGAGGTCGAAGTAGTCG TGCCACCAGTAGCAGTGACAACAGTGAGACATTTGTGGGCAACAGCAGCAGCAACCACAGTGCACTGCAC AGTTTAGTTTCCAGCCTGAAACAGGAAATGACCAAACAGAAGATAGAGTATGAGTCCAGGATAAAGAGCT TAGAACAGCGAAACTTGACTTTGGAAACAGAAATGATGAGCCTCCATGATGAACTGGATCAGGAGAGGAA AAAGTTCACAATGATAGAAATAAAAATGCGAAATGCCGAGCGAGCAAAAGAAGATGCCGAGAAAAGAAAT GACATGCTACAGAAAGAAATGGAGCAGTTTTTTTCCACGTTTGGAGAACTGACAGTGGAACCCAGGAGAA CCGAGAGAGGAAACACAATATGGATTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239254 representing NM_001287805
Red=Cloning site Green=Tags(s) MFVQGGDRDRMTANHESYLLMASTQNDMEDWVKSIRRVIWGPFGGGIFGQKLEDTVRYEKRYGNRLAPML VEQCVDFIRQRGLKEEGLFRLPGQANLVKELQDAFDCGEKPSFDSNTDVHTVASLLKLYLRELPEPVIPY AKYEDFLSCAKLLSKEEEAGVKELAKQVKSLPVVNYNLLKYICRFLDEVQSYSGVNKMSVQNLATVFGPN ILRPKVEDPLTIMEGTVVVQQLMSVMISKHDCLFPKDAELQSKPQDGVSNNNEIQKKATMGQLQNKENNN TKDSPSRQCSWDKSESPQRSSMNNGSPTALSGSKTNSPKNSVHKLDVSRSPPLMVKKNPAFNKGSGIVTN GSFSSSNAEGLEKTQTTPNGSLQARRSSSLKVSGTKMGTHSVQNGTVRMGILNSDTLGNPTNVRNMSWLP NGYVTLRDNKQKEQAGELGQHNRLSTYDNVHQQFSMMNLDDKQSIDSATWSTSSCEISLPENSNSCRSST TTCPEQDFFGGNFEDPVLDGPPQDDLSHPRDYESKSDHRSVGGRSSRATSSSDNSETFVGNSSSNHSALH SLVSSLKQEMTKQKIEYESRIKSLEQRNLTLETEMMSLHDELDQERKKFTMIEIKMRNAERAKEDAEKRN DMLQKEMEQFFSTFGELTVEPRRTERGNTIWIQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001287805 |
ORF Size | 1989 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001287805.2 |
RefSeq Size | 4010 bp |
RefSeq ORF | 1992 bp |
Locus ID | 83478 |
MW | 74.9 kDa |
Gene Summary | This gene encodes a Rho-GTPase activating protein, which is specific for the small GTPase family member Rac. Binding of the encoded protein by filamin A targets it to sites of membrane protrusion, where it antognizes Rac. This results in suppression of lamellae formation and promotion of retraction to regulate cell polarity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016] |
Documents
Product Manuals |
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Resources
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