EYA4 (NM_001301013) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CMD1J; DFNA10
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239190 representing NM_001301013 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGACTCCCAGGATTTAAATGAACAATCAGTAAAGAAAACGTGCACAGAATCAGATGTTTCACAAT CTCAGAATTCCAGGTCTATGGAAATGCAGGACCTAGCAAGTCCTCATACTCTTGTTGGAGGTGGTGATAC TCCAGGTAGCTCCAAACTGGAAAAATCTAATCTCAGCAGCACATCAGTTACTACAAATGGGACAGGAGGG GAAAACATGACTGTTTTAAACACAGCAGACTGGTTGCTGAGTTGCAACACCCCCTCTTCTGCAACAATGT CTCTTCTTGCAGTCAAAACAGAGCCCTTGAACAGCAGTGAAACCACAGCCACGACTGGAGATGGAGCGCT TGACACTTTTACTGGGTCAGTAATTACAAGTAGTGGCTACAGCCCCAGATCAGCACATCAGTATTCCCCA CAGCTGTATCCTTCCAAGCCCTATCCACACATTCTTTCTACACCAGCAGCTCAAACAATGTCTGCCTATG CAGGCCAGACTCAGTATTCGGGGATGCAGCAGCCAGCCGTCTACACAGCCTACTCACAGACAGGACAGCC CTACAGCTTGCCCACTTACGATTTGGGTGTGATGTTGCCAGCCATCAAGACAGAGAGTGGACTTTCCCAA ACTCAGTCCCCATTACAGAGTGGCTGCCTCAGTTACAGCCCAGGGTTCTCTACCCCACAGCCAGGCCAGA CACCTTATTCTTACCAAATGCCAGGTTCTAGTTTTGCACCATCATCTACTATTTATGCAAATAATTCAGT TTCCAATTCAACGAATTTCAGTGGTTCACAACAGGATTATCCATCCTATACAGCCTTTGGCCAAAACCAG TATGCACAGTATTATTCAGCATCAACGTATGGAGCGTATATGACATCGAATAACACAGCCGATGGCACAC CCTCTTCAACCTCTACTTATCAGTTGCAGGAATCTCTCCCAGGACTGACTAACCAACCAGGTACAGATCT TCACCCAGGAGAGTTCGATACCATGCAGAGTCCCTCCACACCCATCAAAGATCTTGATGAGAGAACCTGT AGGAGTTCTGGGTCAAAGTCCAGAGGAAGAGGCCGGAAAAATAATCCCTCCCCGCCTCCTGATAGTGACC TGGAGCGTGTGTTTGTCTGGGATTTGGATGAAACCATCATTGTTTTTCACTCACTGCTCACCGGGTCTTA TGCACAGAAGTATGGCAAGGATCCCCCCATGGCTGTAACCCTTGGACTCCGCATGGAAGAAATGATTTTT AATCTTGCTGATACTCATTTGTTTTTTAATGATTTAGAGGAGTGTGATCAAGTTCATATAGATGATGTTT CCTCTGATGATAATGGGCAGGACTTAAGTACCTACAGTTTTGCAACTGATGGCTTCCATGCAGCTGCAAG TAGTGCAAACCTTTGTTTGCCAACAGGTGTAAGAGGAGGGGTTGACTGGATGAGGAAGTTGGCTTTTCGT TACAGAAGAGTAAAAGAATTATATAACACCTACAAGAACAACGTTGGAGGACTCCTTGGCCCTGCCAAGA GGGATGCCTGGCTACAGTTAAGGGCAGAGATTGAAGGTCTGACAGATTCCTGGCTAACAAATGCACTTAA GTCTTTATCAATTATTAGCACTAGGAGTAACTGCATAAATGTCTTGGTAACGACAACTCAACTGATCCCA GCACTTGCGAAGGTTCTACTCTATAGTTTAGGAGGTGCTTTCCCCATTGAGAATATTTACAGTGCAACTA AAATAGGAAAAGAAAGTTGCTTTGAACGAATAATGCAAAGGTTTGGCAGAAAAGTAGTGTATGTTGTAAT TGGGGATGGTGTAGAAGAAGAACAGGCAGCAAAAAAGCACAACATGCCCTTCTGGAGGATATCCAGTCAC TCAGACCTCCTGGCTCTCCACCAAGCACTGGAATTAGAGTATTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239190 representing NM_001301013 Red=Cloning site Green=Tags(s) MEDSQDLNEQSVKKTCTESDVSQSQNSRSMEMQDLASPHTLVGGGDTPGSSKLEKSNLSSTSVTTNGTGG ENMTVLNTADWLLSCNTPSSATMSLLAVKTEPLNSSETTATTGDGALDTFTGSVITSSGYSPRSAHQYSP QLYPSKPYPHILSTPAAQTMSAYAGQTQYSGMQQPAVYTAYSQTGQPYSLPTYDLGVMLPAIKTESGLSQ TQSPLQSGCLSYSPGFSTPQPGQTPYSYQMPGSSFAPSSTIYANNSVSNSTNFSGSQQDYPSYTAFGQNQ YAQYYSASTYGAYMTSNNTADGTPSSTSTYQLQESLPGLTNQPGTDLHPGEFDTMQSPSTPIKDLDERTC RSSGSKSRGRGRKNNPSPPPDSDLERVFVWDLDETIIVFHSLLTGSYAQKYGKDPPMAVTLGLRMEEMIF NLADTHLFFNDLEECDQVHIDDVSSDDNGQDLSTYSFATDGFHAAASSANLCLPTGVRGGVDWMRKLAFR YRRVKELYNTYKNNVGGLLGPAKRDAWLQLRAEIEGLTDSWLTNALKSLSIISTRSNCINVLVTTTQLIP ALAKVLLYSLGGAFPIENIYSATKIGKESCFERIMQRFGRKVVYVVIGDGVEEEQAAKKHNMPFWRISSH SDLLALHQALELEYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001301013
ORF Size	1935 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301013.1, NP_001287942.1
RefSeq Size	5573 bp
RefSeq ORF	1938 bp
Locus ID	2070
UniProt ID	O95677
Protein Families	Druggable Genome, Phosphatase, Transcription Factors
MW	70.6 kDa
Gene Summary	This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator through its protein phosphatase activity, and it may be important for eye development, and for continued function of the mature organ of Corti. Mutations in this gene are associated with postlingual, progressive, autosomal dominant hearing loss at the deafness, autosomal dominant non-syndromic sensorineural 10 locus. The encoded protein is also a putative oncogene that mediates DNA repair, apoptosis, and innate immunity following DNA damage, cellular damage, and viral attack. Defects in this gene are also associated with dilated cardiomyopathy 1J. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]

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