PHF19 (NM_001286840) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MTF2L1; PCL3; TDRD19B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239045 representing NM_001286840 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGTCTTGGTAATCCGTGGTCCCTATCCCAGCGCCCAGTGTCAGGGGAAGCTGATGGAGAATCGAG CTCTGGATCCAGGGACTCGGGACTCCTATGGTGCCACCAGCCACCTCCCCAACAAGGGGGCCCTGGCGAA GGTCAAGAACAACTTCAAAGACTTGATGTCCAAACTGACGGAGGGCCAGTATGTGCTGTGCCGGTGGACA GATGGCCTGTACTACCTCGGGAAGATCAAGAGGGTCAGCAGCTCTAAGCAAAGCTGCCTCGTGACTTTCG AAGATAATTCCAAATACTGGGTCCTATGGAAGGACATACAGCATGCCGGTGTTCCAGGAGAGGAGCCCAA GTGCAACATCTGCCTAGGGAAGACATCAGGGCCGCTGAATGAGATCCTCATCTGCGGGAAGTGTGGCCTG GGTTACCACCAGCAGTGCCACATCCCCATAGCGGGCAGTGCTGACCAGCCCCTGCTCACACCTTGGTTCT GCCGACGCTGCATCTTCGCACTGGCTGTGCGGAAAGGCGGCGCGCTGAAGAAGGGCGCCATCGCCAGGAC GCTGCAGGCCGTGAAGATGGTGCTGTCCTACCAGCCCGAGGAGCTCGAGTGGGACTCGCCCCATCGCACC AACCAGCAGCAATGCTACTGCTACTGCGGCGGGCCCGGAGAATGGTACCTGCGGATGCTGCAATGTTACC GGTGCAGGCAGTGGTTCCACGAGGCCTGCACCCAGTGCCTCAATGAGCCCATGATGTTTGGAGACCGGTT TTACCTGTTCTTCTGCTCCGTGTGTAACCAGGGCCCAGAGTACATCGAGAGGCTGCCCCTGCGATGGGTG GATGTGGTTCACCTGGCCCTCTATAATCTGGGGGTACAGAGCAAGAAGAAGTACTTTGACTTTGAGGAGA TTCTGGCCTTTGTCAACCACCACTGGGAGCTCCTGCAGCTTGGCAAGCTCACCAGCACCCCAGTGACAGA TCGAGGACCACATCTCCTCAACGCTCTGAACAGTTATAAAAGCCGGTTCCTCTGCGGCAAGGAGATCAAG AAGAAGAAGTGCATCTTCCGCCTGCGCATCCGCGTCCCACCCAACCCGCCAGGGAAGCTGCTGCCTGACA AAGGACTGCTGCCAAATGAGAACAGCGCCTCCTCTGAGCTGCGTAAGAGAGGAAAGAGCAAGCCTGGTTT GTTGCCTCACGAATTCCAGCAGCAGAAAAGGCGAGTTTATAGAAGAAAAAGATCAAAGTTTTTGCTGGAA GATGCTATTCCCAGTAGTGACTTCACCTCAGCCTGGAGCACCAACCACCACCTGGCTAGCATATTTGACT TCACGCTGGATGAAATTCAAAGTTTAAAAAGTGCCAGCTCAGGCCAGACCTTCTTCTCAGATGTCGACTC CACCGACGCTGCCAGCACCTCTGGCTCTGCCTCCACCAGCCTCTCCTATGACTCCAGATGGACAGTGGGC AGCCGAAAGAGGAAGCTGGCAGCCAAGGCATACATGCCCCTGCGGGCAAAGCGGTGGGCAGCTGAGCTGG ATGGACGCTGCCCCTCGGACAGCAGTGCAGAGGGGGCTTCAGTCCCCGAGCGGCCAGACGAAGGCATTGA CAGCCACACATTTGAGAGCATCAGTGAAGATGACTCATCCCTGTCCCACCTCAAGTCATCTATCACCAAC TACTTTGGTGCAGCTGGGCGGTTGGCCTGTGGGGAGAAGTACCAGGTGTTGGCTCGGAGGGTCACACCTG AGGGCAAGGTTCAGTACCTGGTGGAGTGGGAAGGGACCACCCCTTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239045 representing NM_001286840 Red=Cloning site Green=Tags(s) MLVLVIRGPYPSAQCQGKLMENRALDPGTRDSYGATSHLPNKGALAKVKNNFKDLMSKLTEGQYVLCRWT DGLYYLGKIKRVSSSKQSCLVTFEDNSKYWVLWKDIQHAGVPGEEPKCNICLGKTSGPLNEILICGKCGL GYHQQCHIPIAGSADQPLLTPWFCRRCIFALAVRKGGALKKGAIARTLQAVKMVLSYQPEELEWDSPHRT NQQQCYCYCGGPGEWYLRMLQCYRCRQWFHEACTQCLNEPMMFGDRFYLFFCSVCNQGPEYIERLPLRWV DVVHLALYNLGVQSKKKYFDFEEILAFVNHHWELLQLGKLTSTPVTDRGPHLLNALNSYKSRFLCGKEIK KKKCIFRLRIRVPPNPPGKLLPDKGLLPNENSASSELRKRGKSKPGLLPHEFQQQKRRVYRRKRSKFLLE DAIPSSDFTSAWSTNHHLASIFDFTLDEIQSLKSASSGQTFFSDVDSTDAASTSGSASTSLSYDSRWTVG SRKRKLAAKAYMPLRAKRWAAELDGRCPSDSSAEGASVPERPDEGIDSHTFESISEDDSSLSHLKSSITN YFGAAGRLACGEKYQVLARRVTPEGKVQYLVEWEGTTPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286840
ORF Size	1797 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286840.1, NP_001273769.1
RefSeq Size	4181 bp
RefSeq ORF	1800 bp
Locus ID	26147
UniProt ID	Q5T6S3
Protein Families	Druggable Genome
MW	68.1 kDa
Gene Summary	Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases RIOX1 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing. Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal. Also binds dimethylated at 'Lys-36' (H3K36me2). Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter.[UniProtKB/Swiss-Prot Function]

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