ZKSCAN1 (NM_001287054) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	KOX18; PHZ-37; ZNF36; ZNF139; ZSCAN33
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238724 representing NM_001287054 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGGGCAGGATTCCACCCTACAGGACACGCCTCCTCCAGACCCAGAGATATTCCGCCAACGCTTCA GGCGCTTCTGTTACCAGAACACTTTTGGGCCCCGAGAGGCTCTCAGTCGGCTGAAGGAACTTTGTCATCA GTGGCTGCGGCCAGAAATAAACACCAAGGAACAGATCCTGGAGCTTCTGGTGCTAGAGCAGTTTCTTTCC ATCCTGCCCAAGGAGCTCCAGGTCTGGCTGCAGGAATACCGCCCCGATAGTGGAGAGGAGGCCGTGACCC TTCTAGAAGACTTGGAGCTTGATTTATCAGGACAACAGGTCCCAGGTCAAGTTCATGGACCTGAGATGCT CGCAAGGGGGATGGTGCCTCTGGATCCAGTTCAGGAGTCCTCGAGCTTTGACCTTCATCACGAGGCCACC CAGTCCCACTTCAAACATTCGTCTCGGAAACCCCGCCTCTTACAGTCACGAGCTCTTCCTGCTGCCCACA TTCCTGCACCCCCTCATGAGGGTAGTCCCAGAGACCAGGCGATGGCATCTGCACTATTCACAGCGGATTC CCAGGCAATGGTGAAGATCGAGGACATGGCTGTGTCCCTCATTCTGGAGGAATGGGGATGTCAGAATCTG GCTCGGAGGAATCTCAGTAGGGACAACAGGCAGGAGAATTATGGGAGCGCATTTCCCCAGGGTGGTGAAA ACAGGAATGAGAACGAGGAGTCAACCTCAAAGGCTGAAACCTCGGAAGATTCAGCATCACGCGGGGAGAC AACAGGAAGATCCCAGAAAGAGTTTGGAGAGAAACGTGACCAGGAGGGCAAAACAGGAGAAAGACAGCAG AAAAACCCTGAGGAGAAAACCAGGAAAGAGAAAAGAGATTCAGGGCCAGCTATAGGAAAGGACAAAAAAA CCATCACAGGAGAGAGAGGTCCAAGGGAGAAGGGGAAAGGATTGGGAAGAAGCTTCAGTCTGAGCTCCAA CTTCACCACCCCTGAAGAAGTTCCCACGGGAACAAAGTCTCACAGATGTGATGAATGTGGTAAATGCTTC ACGAGAAGTTCAAGCCTTATCCGCCATAAAATAATCCACACTGGAGAAAAGCCCTATGAATGTAGTGAGT GTGGGAAAGCCTTCAGTCTTAACTCCAACCTTGTCCTGCATCAGAGGATCCACACAGGAGAGAAACCTCA TGAATGTAACGAGTGTGGCAAGGCCTTCAGCCACAGTTCCAATCTCATCCTCCATCAGCGCATCCACTCT GGAGAGAAACCTTATGAATGTAATGAGTGCGGGAAGGCCTTCAGCCAGAGCTCGGACCTCACCAAGCATC AGAGAATTCACACGGGGGAGAAACCCTATGAATGTAGTGAATGTGGAAAAGCTTTCAACCGAAACTCATA CCTGATTTTGCATCGGAGAATTCACACTCGAGAAAAGCCCTACAAGTGCACTAAGTGTGGCAAGGCCTTC ACCCGCAGCTCCACCCTCACTCTGCATCACAGAATCCATGCCAGAGAGAGAGCCTCTGAGTACAGCCCAG CCTCCCTTGATGCATTTGGCGCGTTCCTGAAAAGTTGTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238724 representing NM_001287054 Red=Cloning site Green=Tags(s) MWGQDSTLQDTPPPDPEIFRQRFRRFCYQNTFGPREALSRLKELCHQWLRPEINTKEQILELLVLEQFLS ILPKELQVWLQEYRPDSGEEAVTLLEDLELDLSGQQVPGQVHGPEMLARGMVPLDPVQESSSFDLHHEAT QSHFKHSSRKPRLLQSRALPAAHIPAPPHEGSPRDQAMASALFTADSQAMVKIEDMAVSLILEEWGCQNL ARRNLSRDNRQENYGSAFPQGGENRNENEESTSKAETSEDSASRGETTGRSQKEFGEKRDQEGKTGERQQ KNPEEKTRKEKRDSGPAIGKDKKTITGERGPREKGKGLGRSFSLSSNFTTPEEVPTGTKSHRCDECGKCF TRSSSLIRHKIIHTGEKPYECSECGKAFSLNSNLVLHQRIHTGEKPHECNECGKAFSHSSNLILHQRIHS GEKPYECNECGKAFSQSSDLTKHQRIHTGEKPYECSECGKAFNRNSYLILHRRIHTREKPYKCTKCGKAF TRSSTLTLHHRIHARERASEYSPASLDAFGAFLKSCV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001287054
ORF Size	1581 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287054.3
RefSeq Size	9473 bp
RefSeq ORF	1584 bp
Locus ID	7586
Protein Families	Transcription Factors
MW	60.1 kDa
Gene Summary	This gene encodes a member of the Kruppel C2H2-type zinc-finger family of proteins. This encoded protein may function as a transcription factor that regulates the expression of GABA type-A receptors in the brain. Transcripts from this gene have been shown to form stable and abundant circular RNAs. Elevated expression of this gene has been observed in gastric cancer and the encoded protein may stimulate migration and invasion of human gastric cancer cells. [provided by RefSeq, Oct 2016]

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