WASF3 (NM_001291965) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Brush-1; SCAR3; WAVE3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238581 representing NM_001291965 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTTAGTGAAGAGGAACATTGAGCCCCGGCACTTGTGCCGGGGAGCTCTGCCTGAAGGGATTACCA GCGAACTTGAATGTGTAACCAATAGTACTCTTGCCGCTATCATACGCCAGCTGAGCAGTCTGAGCAAACA TGCTGAAGACATATTTGGTGAGTTGTTTAATGAGGCTAACAACTTCTACATCAGAGCAAATTCTCTTCAA GACAGAATTGATCGCCTTGCTGTCAAAGTCACCCAGCTGGATTCAACAGTGGAAGAGGTCTCACTACAGG ATATCAACATGAAAAAAGCTTTCAAAAGTTCCACAGTCCAAGACCAGCAAGTGGTTTCAAAGAACAGCAT TCCTAATCCTGTTGCTGATATTTACAACCAGAGTGATAAGCCACCGCCTCTGAACATCCTGACACCATAC AGAGATGACAAGAAGGATGGGCTGAAGTTCTATACTGATCCTTCCTATTTCTTTGACCTCTGGAAAGAAA AAATGCTACAGGACACAGAAGACAAAAGGAAAGAGAAAAGGCGTCAAAAGAGAGAGAAACACAAGCTGAA CCCTAACAGAAACCAGCAAGTAAATGTGAGAAAAGTCAGAACAAGAAAAGAAGAGTGGGAGAGAAGGAAA ATGGGCATTGAGTTTATGAGTGACGCAAAGAAACTGGAGCAGGCAGGGAGCGCCAAAGAGGACAGAGTGC CCAGCGGGTCACATGCATCGGACGTTACGGATTACTCTTACCCGGCTACTCCCAACCATTCTCTGCACCC CCAGCCTGTGACCCCTTCCTATGCAGCTGGTGACGTGCCACCACACGGGCCTGCAAGCCAGGCTGCGGAG CATGAGTACCGGCCCCCATCTGCCTCGGCGAGGCACATGGCCCTCAACAGACCTCAGCAGCCGCCCCCCC CGCCTCCCCCTCAGGCCCCAGAGGGGTCCCAGGCCTCTGCACCGATGGCTCCAGCAGACTACGGGATGCT CCCAGCGCAGATAATTGAGTATTACAACCCATCCGGACCACCTCCTCCGCCACCTCCTCCTGTGATTCCC TCAGCACAAACTGCCTTCGTCAGCCCTCTCCAGATGCCCATGCAGCCCCCGTTCCCTGCATCAGCCAGCT CCACGCACGCAGCTCCTCCTCACCCACCCTCCACCGGGCTCCTGGTCACAGCCCCGCCACCCCCGGGCCC ACCACCTCCCCCGCCAGGCCCTCCTGGTCCCGGGTCTTCTCTTTCGTCCTCCCCAATGCATGGCCCCCCA GTAGCTGAGGCGAAGCGGCAAGAGCCTGCACAGCCACCAATCAGTGATGCTCGAAGCGACCTCCTCGCTG CTATTCGAATGGGAATTCAACTGAAAAAGGTGCAGGAGCAGCGGGAGCAGGAGGCCAAGCGGGAGCCAGT GGGGAATGACGTGGCCACGATCCTGTCCCGGCGCATTGCCGTGGAGTACAGCGACTCTGACGACGACTCA GAGTTCGACGAGAACGACTGGTCCGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238581 representing NM_001291965 Red=Cloning site Green=Tags(s) MPLVKRNIEPRHLCRGALPEGITSELECVTNSTLAAIIRQLSSLSKHAEDIFGELFNEANNFYIRANSLQ DRIDRLAVKVTQLDSTVEEVSLQDINMKKAFKSSTVQDQQVVSKNSIPNPVADIYNQSDKPPPLNILTPY RDDKKDGLKFYTDPSYFFDLWKEKMLQDTEDKRKEKRRQKREKHKLNPNRNQQVNVRKVRTRKEEWERRK MGIEFMSDAKKLEQAGSAKEDRVPSGSHASDVTDYSYPATPNHSLHPQPVTPSYAAGDVPPHGPASQAAE HEYRPPSASARHMALNRPQQPPPPPPPQAPEGSQASAPMAPADYGMLPAQIIEYYNPSGPPPPPPPPVIP SAQTAFVSPLQMPMQPPFPASASSTHAAPPHPPSTGLLVTAPPPPGPPPPPPGPPGPGSSLSSSPMHGPP VAEAKRQEPAQPPISDARSDLLAAIRMGIQLKKVQEQREQEAKREPVGNDVATILSRRIAVEYSDSDDDS EFDENDWSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291965
ORF Size	1497 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291965.1, NP_001278894.1
RefSeq Size	4841 bp
RefSeq ORF	1500 bp
Locus ID	10810
UniProt ID	Q9UPY6
Protein Families	Druggable Genome
Protein Pathways	Adherens junction, Fc gamma R-mediated phagocytosis
MW	55.5 kDa
Gene Summary	This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. A pseudogene of this gene have been defined on chromosome 6. Alternative splicing results in multiple transcript variants [provided by RefSeq, May 2014]

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