MRPS27 (NM_001286748) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MRP-S27; S27mt
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238173 representing NM_001286748 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCTCCATAGTGCGGCGCGGGATGCTCCTGGCGCGGCAAGTGGTTCTTCCTCAGCTCTCTCCTG CAGGTAAAAGATACCTGCTTTCTTCAGCCTATGTAGACAGCCACAAATGGGAAGCAAGAGAAAAAGAACA TTACTGTCTTGCTGATCTTGCATCTTTAATGGATAAAACATTTGAGAGAAAGTTGCCTGTTAGTTCTTTA ACAATATCACGGCTTATAGACAACATTTCCTCTCGGGAAGAGATAGATCATGCAGAGTATTACCTTTACA AAAAGGGAGAATGCTCATCCAGCAATCCACAGAATTATTCCAGGTTTCGACACAGCCCCAACTGCTGGTA CCTGAGAAACTGGACTATCCACACCTGGATTAGGCAGTGTCTAAAATATGATGCACAAGACAAAGCCCTA TATACCCTTGTAAATAAGGTTCAATATGGAATTTTTCCAGATAACTTTACATTCAATTTACTGATGGATT CTTTCATAAAGAAAGAAAATTACAAAGATGCTTTATCTGTGGTTTTTGAGGTCATGATGCAAGAAGCCTT TGAAGTGCCTTCCACCCAACTTCTCTCCCTCTATGTTTTATTTCATTGCCTGGCAAAGAAGACAGACTTC AGTTGGGAAGAGGAGAGGAACTTTGGTGCATCCCTTTTGCTTCCAGGCCTAAAACAAAAGAACTCAGTGG GTTTCAGTTCCCAGTTGTATGGCTATGCACTTCTTGGGAAGGTGGAGTTGCAGCAAGGGCTACGGGCTGT GTACCACAACATGCCTCTGATATGGAAACCAGGCTACCTTGACAGAGCCCTTCAAGTGATGGAGAAAGTG GCTGCCTCCCCAGAAGACATAAAGCTGTGTAGAGAAGCGCTCGATGTGCTGGGTGCAGTGCTGAAGGCTC TGACTTCAGCTGATGGGGCTTCAGAGGAGCAGTCCCAAAATGATGAAGACAACCAGGGGTCAGAAAAACT GGTGGAGCAGTTAGACATCGAGGAAACAGAGCAGTCCAAGCTTCCTCAATACCTGGAACGATTTAAGGCC TTACATTCTAAGCTTCAAGCTCTGGGCAAAATTGAGTCAGAAGGTCTTTTAAGTCTGACCACCCAGCTTG TCAAGGAAAAACTCTCCACCTGTGAAGCAGAGGACATCGCCACCTATGAGCAGAATCTGCAGCAGTGGCA TCTAGACCTTGTACAGTTGATCCAGAGAGAACAGCAACAGAGGGAGCAAGCGAAGCAGGAGTACCAGGCT CAGAAAGCAGCAAAGGCATCTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238173 representing NM_001286748 Red=Cloning site Green=Tags(s) MAASIVRRGMLLARQVVLPQLSPAGKRYLLSSAYVDSHKWEAREKEHYCLADLASLMDKTFERKLPVSSL TISRLIDNISSREEIDHAEYYLYKKGECSSSNPQNYSRFRHSPNCWYLRNWTIHTWIRQCLKYDAQDKAL YTLVNKVQYGIFPDNFTFNLLMDSFIKKENYKDALSVVFEVMMQEAFEVPSTQLLSLYVLFHCLAKKTDF SWEEERNFGASLLLPGLKQKNSVGFSSQLYGYALLGKVELQQGLRAVYHNMPLIWKPGYLDRALQVMEKV AASPEDIKLCREALDVLGAVLKALTSADGASEEQSQNDEDNQGSEKLVEQLDIEETEQSKLPQYLERFKA LHSKLQALGKIESEGLLSLTTQLVKEKLSTCEAEDIATYEQNLQQWHLDLVQLIQREQQQREQAKQEYQA QKAAKASA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286748
ORF Size	1284 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286748.2
RefSeq Size	2839 bp
RefSeq ORF	1287 bp
Locus ID	23107
UniProt ID	Q92552
MW	49.6 kDa
Gene Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2013]

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