MAEL (NM_001286377) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CT128; SPATA35
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238004 representing NM_001286377 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAACCGTAAGGCCAGCCGGAATGCTTACTATTTCTTCGTGCAGGAGAAGATCCCCGAACTACGGC GACGAGGCCTGCCTGTGGCTCGCGTTGCTGATGCCATCCCTTACTGCTCCTCAGACTGGGCGAAACCTGT TTTCACACCACTGAGGAGGCCAGGCATGCTTGTACCAAAGCAGAATGTTTCACCTCCAGATATGTCAGCT TTGTCTTTAAAAGGTGATCAAGCTCTCCTTGGAGGCATTTTTTATTTTTTGAACATTTTTAGCCATGGCG AGCTACCTCCTCATTGTGAACAGCGCTTCCTCCCTTGTGAAATTGGCTGTGTTAAGTATTCTCTCCAAGA AGGTATTATGGCAGATTTCCACAGTTTTATAAATCCTGGTGAAATTCCACGAGGATTTCGATTTCATTGT CAGGCTGCAAGTGATTCTAGTCACAAGATTCCTATTTCAAATTTTGAACGTGGGCATAACCAAGCAACTG TGTTACAAAACCTTTATAGATTTATTCATCCCAACCCAGGGAACTGGCCACCTATCTACTGCAAGTCTGA TGATAGAACCAGAGTCAACTGGTGTTTGAAGCATATGGCAAAGGCATCAGAAATCAGGCAAGATCTACAA CTTCTCACTGTAGAGGACCTTGTAGTGGGGATCTACCAACAAAAATTTCTCAAGGAGCCCTCTAAGACTT GGATTCGAAGCCTCCTAGATGTGGCCATGTGGGATTATTCTAGCAACACAAGGTGCAAGTGGCATGAAGA AAATGATATTCTCTTCTGTGCTTTAGCTGTTTGCAAGAAGATTGCGTACTGCATCAGTAATTCTCTGGCC ACTCTCTTTGGAATCCAGCTCACAGAGGCTCATGTACCACTACAAGATTATGAGGCCAGCAATAGTGTGA CACCCAAAATGGTTGTATTGGATGCAGGGCGTTACCAGAAGCTAAGGGTTGGGAGTTCAGGATTCTCTCA TTTCAACTCTTCTAATGAGGAACAAAGATCAAACACACCCATTGGTGACTACCCATCTAGGGCAAAAATT TCTGGCCAAAACAGCAGCGTTCGGGGAAGAGGAATTACCCGCTTACTAGAGAGCATTTCCAATTCTTCCA GCAATATCCACAAATTCTCCAACTGTGACACTTCACTCTCACCTTACATGTCCCAAAAAGATGGATACAA ATCTTTCTCTTCCTTATCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238004 representing NM_001286377 Red=Cloning site Green=Tags(s) MPNRKASRNAYYFFVQEKIPELRRRGLPVARVADAIPYCSSDWAKPVFTPLRRPGMLVPKQNVSPPDMSA LSLKGDQALLGGIFYFLNIFSHGELPPHCEQRFLPCEIGCVKYSLQEGIMADFHSFINPGEIPRGFRFHC QAASDSSHKIPISNFERGHNQATVLQNLYRFIHPNPGNWPPIYCKSDDRTRVNWCLKHMAKASEIRQDLQ LLTVEDLVVGIYQQKFLKEPSKTWIRSLLDVAMWDYSSNTRCKWHEENDILFCALAVCKKIAYCISNSLA TLFGIQLTEAHVPLQDYEASNSVTPKMVVLDAGRYQKLRVGSSGFSHFNSSNEEQRSNTPIGDYPSRAKI SGQNSSVRGRGITRLLESISNSSSNIHKFSNCDTSLSPYMSQKDGYKSFSSLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286377
ORF Size	1209 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286377.1, NP_001273306.1
RefSeq Size	1849 bp
RefSeq ORF	1212 bp
Locus ID	84944
UniProt ID	Q96JY0
MW	46 kDa
Gene Summary	Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Its association with piP-bodies suggests a participation in the secondary piRNAs metabolic process. Required for the localization of germ-cell factors to the meiotic nuage (By similarity).[UniProtKB/Swiss-Prot Function]

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