GAS8 (NM_001286205) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CILD33; DRC4; GAS11
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237954 representing NM_001286205 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAAGCCGAGGAGAGGCACCAGGTGGAGATCAAGGTGTACAAGCAGAAAGTGAAGCACCTGCTAT ATGAGCACCAGAACAACCTGACAGAGATGAAGGCTGAGGGCACTGTAGTCATGAAGCTGGCACAGAAAGA GCACCGCATACAGGAGAGTGTGCTGCGCAAGGACATGCGGGCACTGAAGGTGGAGCTCAAGGAGCAGGAG CTGGCCAGTGAGGTGGTGGTGAAGAACCTGCGGCTGAAACACACCGAGGAGATCACCAGGATGCGGAATG ATTTTGAGAGGCAAGTTCGAGAAATTGAGGCCAAGTATGATAAGAAGATGAAGATGCTGAGGGACGAACT CGACTTGCGGAGAAAGACTGAGCTCCACGAAGTGGAGGAGAGGAAGAATGGCCAGATCCACACGCTGATG CAGCGCCACGAGGAGGCCTTCACCGACATTAAGAACTACTACAACGACATCACCCTCAACAACCTGGCCC TCATCAACTCCCTCAAGGAGCAGATGGAGGACATGCGGAAGAAGGAGGACCACCTGGAGAGGGAGATGGC AGAGGTGTCTGGGCAGAACAAGCGCCTGGCAGACCCTCTCCAGAAGGCTCGGGAGGAGATGAGCGAGATG CAGAAACAGCTCGCAAACTACGAGAGGGACAAGCAGATCCTGCTTTGCACAAAAGCCCGTTTGAAAGTCA GGGAGAAAGAGCTGAAAGACCTGCAGTGGGAGCATGAAGTGTTAGAGCAGCGATTCACCAAGGTGCAGCA GGAGCGGGACGAGCTCTATCGGAAGTTCACCGCAGCCATCCAGGAGGTGCAGCAGAAGACAGGGTTCAAG AACCTCGTGCTAGAACGCAAGCTGCAGGCTCTGAGCGCCGCTGTGGAGAAGAAGGAGGTGCAGTTCAACG AGGTCCTGGCTGCCTCTAACCTGGACCCTGCAGCCCTGACGCTGGTGTCCCGCAAGCTGGAGGATGTTCT TGAGTCGAAGAACAGCACCATCAAGGACCTGCAGTATGAGCTGGCCCAGGTCTGTAAGGCCCATAACGAC CTGCTGCGCACGTATGAGGCAAAGCTGCTGGCCTTCGGGATCCCTCTGGACAACGTGGGCTTCAAGCCCT TGGAAACAGCTGTGATCGGACAGACACTGGGCCAGGGCCCCGCGGGACTGGTGGGCACCCCGACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237954 representing NM_001286205 Red=Cloning site Green=Tags(s) MEEAEERHQVEIKVYKQKVKHLLYEHQNNLTEMKAEGTVVMKLAQKEHRIQESVLRKDMRALKVELKEQE LASEVVVKNLRLKHTEEITRMRNDFERQVREIEAKYDKKMKMLRDELDLRRKTELHEVEERKNGQIHTLM QRHEEAFTDIKNYYNDITLNNLALINSLKEQMEDMRKKEDHLEREMAEVSGQNKRLADPLQKAREEMSEM QKQLANYERDKQILLCTKARLKVREKELKDLQWEHEVLEQRFTKVQQERDELYRKFTAAIQEVQQKTGFK NLVLERKLQALSAAVEKKEVQFNEVLAASNLDPAALTLVSRKLEDVLESKNSTIKDLQYELAQVCKAHND LLRTYEAKLLAFGIPLDNVGFKPLETAVIGQTLGQGPAGLVGTPT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286205
ORF Size	1185 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286205.1, NP_001273134.1
RefSeq Size	3308 bp
RefSeq ORF	1188 bp
Locus ID	2622
UniProt ID	O95995
MW	46.8 kDa
Gene Summary	This gene includes 11 exons spanning 25 kb and maps to a region of chromosome 16 that is sometimes deleted in breast and prostrate cancer. The second intron contains an apparently intronless gene, C16orf3, that is transcribed in the opposite orientation. This gene is a putative tumor suppressor gene. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.