LNK (SH2B3) (NM_001291424) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	IDDM20; LNK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237815 representing NM_001291424 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGATTCCCGGTGCTGTGCCGTGGCTGGAGGAGGACCTGGCCTGGCTGTGCCAGTTGGCTGGGAGCCC CTCCACCCCAGAAGCCCTTGCTCTCACCTCTTCTTCTGAATCATCGTTCTTCGAAGAGTTCAAGGCCCAA GCTACAAGCAGCTTGCTCCAGCATCCAGGAGGTCCGGTGGTGCACACGGCTTGAGATGCCTGACAACCTT TACACCTTTGTGCTGAAGGTGAAGGACCGGACAGACATCATCTTTGAGGTGGGAGACGAGCAGCAGCTGA ATTCATGGATGGCTGAGCTCTCGGAGTGCACAGGCCGAGGGCTGGAGAGCACAGAAGCAGAGATGCATAT TCCCTCAGCCCTAGAGCCTAGCACGTCCAGCTCCCCAAGGGGCAGCACAGATTCCCTTAACCAAGGTGCT TCTCCTGGGGGGCTGCTGGACCCGGCCTGCCAGAAGACGGACCATTTCCTGTCCTGCTACCCCTGGTTCC ACGGCCCCATCTCCAGAGTGAAAGCAGCTCAGCTGGTTCAGCTGCAGGGCCCTGATGCTCATGGAGTGTT CCTGGTGCGGCAGAGCGAGACGCGGCGTGGGGAATACGTGCTCACTTTCAACTTTCAGGGGATAGCCAAG CACCTGCGCCTGTCGCTGACAGAGCGGGGCCAGTGCCGTGTGCAGCACCTCCACTTTCCCTCGGTCGTGG ACATGCTCCACCACTTCCAGCGCTCGCCCATCCCACTCGAGTGCGGCGCCGCCTGTGATGTCCGGCTCTC CAGCTACGTGGTAGTCGTCTCCCAACCACCAGGTTCCTGCAACACGGTCCTCTTCCCTTTCTCCCTTCCT CACTGGGATTCAGAGTCCCTTCCTCACTGGGGTTCAGAGTTGGGCCTTCCCCACCTTAGTTCTTCTGGCT GTCCCCGGGGGCTCAGCCCAGAGGGTCTCCCAGGGCGATCCTCACCCCCCGAGCAGATCTTCCACCTGGT GCCTTCGCCCGAAGAACTGGCCAACAGCCTGCAGCACCTGGAGCATGAGCCTGTGAATCGAGCCCGGGAC TCGGACTACGAAATGGACTCATCCTCCCGGAGCCACCTGCGGGCCATAGACAATCAGTACACACCTCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237815 representing NM_001291424 Red=Cloning site Green=Tags(s) MGFPVLCRGWRRTWPGCASWLGAPPPQKPLLSPLLLNHRSSKSSRPKLQAACSSIQEVRWCTRLEMPDNL YTFVLKVKDRTDIIFEVGDEQQLNSWMAELSECTGRGLESTEAEMHIPSALEPSTSSSPRGSTDSLNQGA SPGGLLDPACQKTDHFLSCYPWFHGPISRVKAAQLVQLQGPDAHGVFLVRQSETRRGEYVLTFNFQGIAK HLRLSLTERGQCRVQHLHFPSVVDMLHHFQRSPIPLECGAACDVRLSSYVVVVSQPPGSCNTVLFPFSLP HWDSESLPHWGSELGLPHLSSSGCPRGLSPEGLPGRSSPPEQIFHLVPSPEELANSLQHLEHEPVNRARD SDYEMDSSSRSHLRAIDNQYTPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291424
ORF Size	1119 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291424.1, NP_001278353.1
RefSeq Size	4500 bp
RefSeq ORF	1122 bp
Locus ID	10019
UniProt ID	Q9UQQ2
Protein Pathways	Neurotrophin signaling pathway
MW	41.9 kDa
Gene Summary	This gene encodes a member of the SH2B adaptor family of proteins, which are involved in a range of signaling activities by growth factor and cytokine receptors. The encoded protein is a key negative regulator of cytokine signaling and plays a critical role in hematopoiesis. Mutations in this gene have been associated with susceptibility to celiac disease type 13 and susceptibility to insulin-dependent diabetes mellitus. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

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