Archaemetzincin 2 (AMZ2) (NM_001289054) Human Tagged ORF Clone
CAT#: RC237716
- TrueORF®
AMZ2 (myc-DDK-tagged) - Human archaelysin family metallopeptidase 2 (AMZ2), transcript variant 7
ORF Plasmid: tGFP
"NM_001289054" in other vectors (2)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC237716 representing NM_001289054.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAAATAATACGGCACTCCGAACAGACACTAAAAACAGCTCTCATCTCAAAGAACCCAGTGCTTGTA TCACAGTATGAGAAATTAGATGCTGGGGAACAACGTTTAATGAATGAAGCCTTCCAGCCAGCCAGTGAT CTCTTTGGACCCATTACCTTGCATTCTCCATCAGATTGGATCACCTCCCACCCTGAGGCTCCCCAAGAC TTTGAACAGTTCTTCAGTGATCCTTACAGAAAGACACCCTCTCCAAACAAACGCAGCATTTATATACAG TCCATTGGCTCTCTAGGAAACACCAGAATTATCAGTGAAGAATATATTAAATGGCTCACGGGCTACTGT AAAGCATATTTCTATGGCTTGAGAGTAAAACTCCTAGAACCAGTTCCTGTTTCTGTAACAAGATGTTCC TTTAGAGTCAATGAGAACACACACAACCTACAAATTCATGCAGGGGACATCCTGAAGTTCTTGAAAAAG AAGAAACCTGAAGATGCCTTCTGTGTTGTGGGAATAACAATGATTGATCTTTACCCAAGAGACTCGTGG AATTTTGTCTTTGGACAGGCCTCTTTGACAGATGGTGTGGGGATATTCAGCTTTGCCAGGTATGGCAGT GATTTTTATAGCATGCACTATAAAGGCAAAGTGAAGAAGCTCAAGAAAACATCTTCAAGTGACTATTCA ATTTTCGACAACTATTATATTCCAGAAATAACTAGTGTTTTACTACTTCGATCCTGTAAGACTTTAACC CATGAGATCGGACACATATTTGGACTGCGACACTGCCAGTGGCTTGCATGCCTCATGCAAGGCTCCAAC CACTTGGAAGAAGCTGACCGGCGCCCTCTAAACCTTTGCCCTATCTGTTTGCACAAGTTGCAGTGTGCT GTTGGCTTCAGCATTGTAGAAAGATACAAAGCACTGGTGAGGTGGATTGATGATGAATCTTCTGACACA CCTGGAGCAACTCCAGAACACAGTCACGAGGATAATGGGAATTTACCGAAACCCGTGGAAGCCTTTAAG GAATGGAAAGAGTGGATAATAAAATGCCTGGCAGTTCTCCAAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC237716
Blue=ORF Red=Cloning site Green=Tag(s) MQIIRHSEQTLKTALISKNPVLVSQYEKLDAGEQRLMNEAFQPASDLFGPITLHSPSDWITSHPEAPQD FEQFFSDPYRKTPSPNKRSIYIQSIGSLGNTRIISEEYIKWLTGYCKAYFYGLRVKLLEPVPVSVTRCS FRVNENTHNLQIHAGDILKFLKKKKPEDAFCVVGITMIDLYPRDSWNFVFGQASLTDGVGIFSFARYGS DFYSMHYKGKVKKLKKTSSSDYSIFDNYYIPEITSVLLLRSCKTLTHEIGHIFGLRHCQWLACLMQGSN HLEEADRRPLNLCPICLHKLQCAVGFSIVERYKALVRWIDDESSDTPGATPEHSHEDNGNLPKPVEAFK EWKEWIIKCLAVLQK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001289054 |
ORF Size | 1080 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 1407 bp |
RefSeq ORF | 1083 bp |
Locus ID | 51321 |
UniProt ID | Q86W34 |
Protein Families | Druggable Genome |
MW | 41.3 kDa |
Gene Summary | The protein encoded by this gene is a zinc metalloprotease that displays some activity against angiotensin-3. The encoded protein is inhibited by the aminopeptidase inhibitor amastatin, as well as by the general inhibitors o-phenanthroline and batimastat. Defects in this gene may be associated with lung tumorigenesis. [provided by RefSeq, Oct 2016] |
Documents
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Resources
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