MAEA (NM_001297433) Human Tagged ORF Clone
CAT#: RC237623
- TrueORF®
MAEA (myc-DDK-tagged) - Human macrophage erythroblast attacher (MAEA), transcript variant 6
ORF Plasmid: tGFP
"NM_001297433" in other vectors (2)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | EMLP; EMP; GID9; HLC-10; P44EMLP; PIG5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC237623 representing NM_001297433
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGTGGCCGAGCTGGAGAAGACGTTGAGCGGCTGCCCCGCCGTGGACTCCGTGGTCAGCCTGCTGG ACGGCGTGGTGGAGAAGCTCAGCGTCCTCAAGAGGAAGGCGGTGGAATCCATCCAGGCCGAGGACGAGAG CGCCAAGCTGTGCAAGCGCCGGATCGAGCACCTCAAAGAGCATAGCAGCGACCAGCCCGCGGCGGCCAGC GTGTGGAAGAGGAAGCGCATGGATCGCATGATGGTGGAGCACCTGCTGCGTTGCGGCTACTACAACACGG CTGTCAAGCTGGCGCGCCAGAGCGGCATCGAGGACCTAGTGAATATTGAGATGTTCCTGACGGCCAAAGA GGTGGAGGAGTCCCTGGAGAGGCGTGAGACGGCCACCTGCCTGGCCTGGTGCCATGACAACAAGTCCCGG CTCCGGAAGATGAAGAGCTGCCTGGAGTTCAGCCTCAGAATCCAGGAGTTCATTGAACTCATCCGGCAGA ATAAGAGACTGGACGCTGTGAGACATGCAAGAAAGCACTTCAGCCAAGCAGAAGGGAGCCAGCTGGACGA GGTGCGCCAGGCCATGGGCATGCTGGCCTTCCCGCCCGACACGCACATCTCCCCGTACAAGGACCTTCTG GACCCTGCACGGTGGCGGATGCTGATCCAGCAGTTCCGGTACGACAACTACCGACTACACCAGCTGGGAA ACAATTCTGTGTTCACCCTCACCCTGCAGGCTGGCCTCTCAGCCATCAAGACACCACAGTGCTACAAGGA GGACGGCAGCTCCAAGAGCCCTGACTGCCCTGTGTGCAGCCGCTCCCTGAACAAGCTGGCGCAGCCCCTG CCCATGGCCCACTGTGCCAACTCCCGCCTGGTCTGCAAGATTTCTGGCGACGTGATGAACGAGAACAATC CGCCCATGATGCTGCCCAACGGCTACGTCTACGGCTACAATTCTCTGCTTTCTATCCGTCAAGATGATAA AGTCGTGTGCCCGAGAACCAAAGAAGTCTTCCACTTCTCACAAGCCGAGAAGGTGTACATCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC237623 representing NM_001297433
Red=Cloning site Green=Tags(s) MVVAELEKTLSGCPAVDSVVSLLDGVVEKLSVLKRKAVESIQAEDESAKLCKRRIEHLKEHSSDQPAAAS VWKRKRMDRMMVEHLLRCGYYNTAVKLARQSGIEDLVNIEMFLTAKEVEESLERRETATCLAWCHDNKSR LRKMKSCLEFSLRIQEFIELIRQNKRLDAVRHARKHFSQAEGSQLDEVRQAMGMLAFPPDTHISPYKDLL DPARWRMLIQQFRYDNYRLHQLGNNSVFTLTLQAGLSAIKTPQCYKEDGSSKSPDCPVCSRSLNKLAQPL PMAHCANSRLVCKISGDVMNENNPPMMLPNGYVYGYNSLLSIRQDDKVVCPRTKEVFHFSQAEKVYIM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001297433 |
ORF Size | 1044 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001297433.1, NP_001284362.1 |
RefSeq Size | 2324 bp |
RefSeq ORF | 1047 bp |
Locus ID | 10296 |
Protein Families | Druggable Genome |
MW | 40.2 kDa |
Gene Summary | This gene encodes a protein that mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. The encoded protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
Documents
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