GRASP65 (GORASP1) (NM_001278789) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GOLPH5; GRASP65; P65
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237611 representing NM_001278789 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCTGGGCGTCAGCGCTGAGCAGCCCGCAGGCGGCGCCGAGGGCTTCCACCTCCACGGGGATGTGG AACCATCTTCACCTGCTGCCCTTGCCGGCCTGCGCCCCTACACAGACTATGTGGTTGGTTCGGACCAGAT TCTCCAGGAGTCCGAGGACTTCTTTACGCTCATCGAGTCTCATGAGGGGAAGCCCTTGAAGCTGATGGTG TATAACTCCAAGTCAGACTCCTGCCGGGAGGTGACTGTAACTCCCAACGCAGCCTGGGGTGGAGAGGGCA GTCTGGGATGTGGCATTGGCTATGGGTATCTACACCGGATCCCAACTCAGCCCCCCAGCTACCACAAGAA GCCACCTGGCACCCCACCACCTTCTGCTCTACCACTTGGTGCCCCACCACCTGATGCTCTACCACCTGGA CCCACCCCCGAGGACTCTCCTTCCCTGGAGACAGGTTCCAGGCAGAGTGACTACATGGAGGCCCTGCTGC AGGCACCTGGCTCCTCCATGGAGGATCCCCTTCCTGGGCCTGGGAGTCCCAGCCACAGTGCTCCAGACCC TGATGGACTTCCCCATTTCATGGAGACTCCTCTTCAGCCCCCACCTCCAGTGCAGCGAGTTATGGACCCA GGCTTCCTGGACGTGTCGGGAATTTCTCTCTTGGACAACAGCAATGCCAGTGTGTGGCCCAGCCTGCCCT CTTCCACAGAACTGACCACCACAGCTGTCTCAACCTCAGGGCCAGAGGACATCTGCTCCAGCAGCAGTTC TCATGAGCGGGGTGGTGAGGCTACATGGTCTGGGTCAGAGTTTGAGGTCTCCTTCCTGGACAGCCCAGGT GCCCAAGCCCAGGCGGACCACCTGCCTCAGCTGACTCTTCCTGACAGTCTCACCTCTGCAGCCTCACCAG AAGATGGGCTGTCCGCCGAGCTGCTTGAAGCTCAGGCTGAGGAGGAACCAGCAAGCACAGAGGGCCTAGA TACTGGGACGGAGGCTGAGGGGCTGGACAGCCAGGCCCAGATCTCTACCACAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237611 representing NM_001278789 Red=Cloning site Green=Tags(s) MGLGVSAEQPAGGAEGFHLHGDVEPSSPAALAGLRPYTDYVVGSDQILQESEDFFTLIESHEGKPLKLMV YNSKSDSCREVTVTPNAAWGGEGSLGCGIGYGYLHRIPTQPPSYHKKPPGTPPPSALPLGAPPPDALPPG PTPEDSPSLETGSRQSDYMEALLQAPGSSMEDPLPGPGSPSHSAPDPDGLPHFMETPLQPPPPVQRVMDP GFLDVSGISLLDNSNASVWPSLPSSTELTTTAVSTSGPEDICSSSSSHERGGEATWSGSEFEVSFLDSPG AQAQADHLPQLTLPDSLTSAASPEDGLSAELLEAQAEEEPASTEGLDTGTEAEGLDSQAQISTTE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278789
ORF Size	1035 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278789.2
RefSeq Size	3041 bp
RefSeq ORF	1038 bp
Locus ID	64689
UniProt ID	Q9BQQ3
MW	36.2 kDa
Gene Summary	The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.