ZNF101 (NM_001300949) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HZF12
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237400 representing NM_001300949 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGCTCATGCTGGACACAAACGATCTGAGTGTGGTGGGGAATGGAGAGAGACGCCCCGTAAACAGA AACAACATGGGAAAGCCTCCATTTCCCCCAGTAGTGGTGCACGGCGCACAGTAACACCAACTCGAAAGAG ACCTTATGAATGCAAGGTGTGCGGGAAAGCCTTTAATTCTCCCAATTTATTTCAAATCCATCAAAGAACT CACACTGGAAAGAGGTCCTATAAATGTAGGGAAATAGTGAGAGCCTTCACAGTTTCCAGTTTCTTTCGAA AACATGGAAAAATGCATACTGGAGAAAAACGCTATGAATGTAAATACTGTGGAAAACCTATCGATTATCC CAGTTTATTTCAAATTCATGTTAGAACTCACACTGGAGAAAAACCTTACAAATGTAAACAATGTGGTAAA GCCTTCATTTCCGCAGGTTACCTTCGGACACATGAAATCAGATCTCACGCGCTGGAGAAATCCCACCAAT GTCAGGAATGTGGGAAAAAACTCAGTTGTTCCAGTTCCCTTCACAGACATGAAAGAACTCATAGTGGAGG AAAACTCTACGAATGTCAAAAATGTGCCAAAGTCTTTAGATGTCCCACGTCCCTTCAAGCACATGAAAGA GCTCACACTGGAGAAAGACCTTATGAATGTAATAAATGTGGTAAAACCTTCAATTATCCCAGTTGTTTTC GAAGACATAAAAAAACTCATAGTGGAGAAAAGCCATATGAATGTACAAGGTGTGGTAAAGCCTTTGGGTG GTGCAGTTCCCTCCGAAGACATGAAATGACTCACACTGGAGAAAAACCCTTTGATTGTAAACAGTGTGGT AAAGTCTTTACTTTTTCAAATTACCTTAGACTTCATGAAAGAACTCATTTGGCCGGGCGTAGCCAGTGCT TTGGCAGGAGGCAGGGGGATCACCTGAGCCCAGGAGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237400 representing NM_001300949 Red=Cloning site Green=Tags(s) MRAHAGHKRSECGGEWRETPRKQKQHGKASISPSSGARRTVTPTRKRPYECKVCGKAFNSPNLFQIHQRT HTGKRSYKCREIVRAFTVSSFFRKHGKMHTGEKRYECKYCGKPIDYPSLFQIHVRTHTGEKPYKCKQCGK AFISAGYLRTHEIRSHALEKSHQCQECGKKLSCSSSLHRHERTHSGGKLYECQKCAKVFRCPTSLQAHER AHTGERPYECNKCGKTFNYPSCFRRHKKTHSGEKPYECTRCGKAFGWCSSLRRHEMTHTGEKPFDCKQCG KVFTFSNYLRLHERTHLAGRSQCFGRRQGDHLSPGV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001300949
ORF Size	948 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300949.1, NP_001287878.1
RefSeq Size	4581 bp
RefSeq ORF	951 bp
Locus ID	94039
UniProt ID	Q8IZC7
Protein Families	Druggable Genome, Transcription Factors
MW	36.9 kDa
Gene Summary	Zinc finger proteins (ZNFs), such as ZNF101, bind nucleic acids and perform many key functions, the most important of which is regulating transcription (summary by Bellefroid et al., 1993 [PubMed 8467795]). See ZNF91 (MIM 603971) for general information on ZNFs.[supplied by OMIM, Nov 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.