RHEBL1 (NM_001303126) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RHEBL1c
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC236338 representing NM_001303126 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGATGGATGATTTGGATACAACTGTCACACTGCTCCCCACAGGGAAGACATCTTTGGCACATCAAT TTGTGGAAGGCGAGTTCTCGGAAGGCTACGATCCTACAGTGGAGAATACTTACAGCAAGATAGTGACTCT TGGCAAAGATGAGTTTCACCTACATCTGGTGGACACAGCAGGGCAGGATGAGTACAGCATTCTGCCCTAT TCATTCATCATTGGGGTCCATGGTTATGTGCTTGTGTATTCTGTCACCTCTCTGCATAGCTTCCAAGTCA TTGAGAGTCTGTACCAAAAGCTACATGAAGGCCATGGGAAAACCCGGGTGCCAGTGGTTCTAGTGGGGAA CAAGGCAGATCTCTCTCCAGAGAGAGAGGTACAGGCAGTTGAAGGAAAGAAGCTGGCAGAGTCCTGGGGT GCGACATTTATGGAGTCATCTGCTCGAGAGAATCAGCTGACTCAAGGCATCTTCACCAAAGTCATCCAGG AGATTGCCCGTGTGGAGAATTCCTATGGGCAAGAGCGTCGCTGCCATCTCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC236338 representing NM_001303126 Red=Cloning site Green=Tags(s) MWMDDLDTTVTLLPTGKTSLAHQFVEGEFSEGYDPTVENTYSKIVTLGKDEFHLHLVDTAGQDEYSILPY SFIIGVHGYVLVYSVTSLHSFQVIESLYQKLHEGHGKTRVPVVLVGNKADLSPEREVQAVEGKKLAESWG ATFMESSARENQLTQGIFTKVIQEIARVENSYGQERRCHLM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001303126
ORF Size	543 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001303126.2
RefSeq Size	1303 bp
RefSeq ORF	546 bp
Locus ID	121268
UniProt ID	Q8TAI7
MW	20.8 kDa
Gene Summary	Binds GTP and exhibits intrinsic GTPase activity. May activate NF-kappa-B-mediated gene transcription. Promotes signal transduction through MTOR, activates RPS6KB1, and is a downstream target of the small GTPase-activating proteins TSC1 and TSC2.[UniProtKB/Swiss-Prot Function]

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