CLIP170 (CLIP1) (NM_001247997) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CLIP; CLIP-170; CLIP170; CYLN1; RSN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235302 representing NM_001247997 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTATGCTAAAGCCAAGTGGGCTTAAGGCCCCCACCAAGATCCTGAAGCCTGGAAGCACAGCTCTGA AGACACCTACGGCTGTTGTAGCTCCAGTAGAAAAAACCATATCCAGTGAAAAAGCATCAAGCACTCCATC ATCTGAGACTCAGGAGGAATTTGTGGATGACTTTCGAGTTGGGGAGCGAGTTTGGGTGAATGGAAATAAG CCTGGATTTATCCAGTTTCTTGGAGAAACCCAGTTTGCACCAGGCCAGTGGGCTGGAATTGTTTTAGATG AACCCATAGGCAAGAACGATGGTTCGGTGGCAGGAGTTCGGTATTTCCAGTGTGAACCTTTAAAGGGCAT ATTTACCCGACCTTCAAAGTTAACAAGGAAGGTGCAAGCAGAAGATGAAGCTAATGGCCTGCAGACAACG CCCGCCTCCCGAGCTACTTCACCGCTGTGCACTTCTACGGCCAGCATGGTGTCTTCCTCCCCCTCCACCC CTTCAAACATCCCTCAGAAACCATCACAGCCAGCAGCAAAGGAACCTTCAGCTACGCCTCCGATCAGCAA CCTTACAAAAACTGCCAGTGAATCTATCTCCAACCTTTCAGAGGCTGGCTCAATCAAGAAAGGAGAAAGA GAGCTCAAAATCGGAGACAGAGTATTGGTTGGTGGCACTAAGGCTGGTGTAGTCCGGTTTCTTGGGGAGA CCGACTTTGCCAAGGGGGAGTGGTGTGGCGTGGAGTTAGATGAGCCACTTGGGAAGAATGATGGCGCTGT TGCTGGAACAAGGTATTTTCAGTGTCAACCCAAATATGGCTTGTTCGCTCCTGTCCACAAAGTTACCAAG ATTGGCTTCCCTTCCACTACACCAGCCAAAGCCAAGGCCAACGCAGTGAGGCGAGTGATGGCGACCACGT CCGCCAGCCTGAAGCGCAGCCCTTCTGCCTCTTCCCTCAGCTCCATGAGCTCAGTGGCCTCCTCTGTGAG CAGCAGGCCCAGTCGGACAGGACTATTGACTGAAACCTCCTCCCGTTACGCCAGGAAGATCTCCGGTACC ACTGCCCTCCAGGAGGCCCTGAAGGAGAAGCAGCAGCACATTGAGCAGCTGCTGGCGGAACGGGATCTGG AGAGGGCGGAGGTGGCCAAGGCCACGAGCCACGTGGGGGAGATAGAGCAGGAGCTAGCTCTGGCCCGGGA CGGACATGACCAGCATGTCCTGGAATTGGAAGCCAAAATGGACCAGCTGCGAACAATGGTGGAAGCTGCT GACAGGGAGAAGGTGGAGCTTCTCAACCAGCTTGAAGAGGAGAAAAGGAAGGTTGAGGACCTTCAGTTCC GGGTTGAAGAAGAATCAATTACCAAAGGTGATCTTGAGCAAAAGAGCCAGATTTCTGAAGATCCTGAGAA TACGCAGACCAAACTGGAGCATGCCCGCATTAAGGAGCTTGAACAGAGCCTGCTCTTTGAAAAGACCAAA GCTGACAAACTCCAGAGGGAGTTAGAAGACACTAGGGTGGCTACAGTTTCAGAAAAGTCACGTATAATGG AACTGGAGAAAGACCTAGCATTGAGAGTACAGGAAGTAGCTGAGCTCCGAAGAAGGCTAGAGTCCAATAA GCCTGCTGGGGATGTGGACATGTCACTTTCCCTTTTGCAAGAGATAAGCTCTTTGCAAGAAAAGTTAGAA GTCACCCGTACTGACCACCAGAGAGAAATAACTTCTCTGAAGGAGCATTTTGGAGCCCGGGAAGAAACTC ATCAGAAGGAGATAAAGGCTCTGTATACCGCCACGGAAAAGCTTTCCAAAGAGAACGAGTCATTGAAAAG CAAGCTGGAGCATGCCAACAAAGAGAACTCAGATGTGATAGCTCTATGGAAGTCCAAACTGGAGACTGCC ATCGCATCCCACCAGCAGGCGATGGAAGAACTGAAGGTATCTTTCAGCAAAGGGCTTGGAACAGAGACGG CAGAATTTGCTGAACTAAAAACACAAATAGAGAAAATGAGACTAGATTACCAACACGAAATAGAAAATTT GCAGAATCAACAAGACTCTGAACGGGCTGCCCATGCTAAAGAGATGGAAGCCTTGAGGGCTAAACTGATG AAAGTTATTAAAGAAAAGGAAAACAGTCTGGAAGCCATCAGGTCGAAACTGGACAAAGCAGAAGACCAGC ATCTCGTAGAAATGGAAGACACGTTAAACAAATTACAGGAAGCTGAAATAAAGGTAAAGGAGCTAGAGGT ACTGCAAGCCAAATGCAATGAACAAACCAAGGTTATTGATAATTTTACATCACAGCTCAAGGCTACTGAA GAAAAGCTCTTGGATCTTGATGCACTTCGGAAAGCCAGTTCCGAAGGTAAATCGGAAATGAAGAAACTTA GACAGCAGCTTGAGGCAGCTGAGAAACAGATTAAACATTTAGAGATTGAAAAGAATGCTGAAAGTAGCAA GGCTAGTAGCATTACCAGAGAGCTCCAGGGGAGAGAGCTAAAGCTTACTAACCTTCAGGAAAATTTGAGT GAAGTCAGTCAAGTGAAAGAGACTTTGGAAAAAGAACTTCAGATTTTGAAAGAAAAGTTTGCTGAAGCTT CAGAGGAGGCAGTCTCTGTTCAGAGAAGTATGCAAGAAACTGTAAATAAGTTACACCAAAAGGAGGAACA GTTTAACATGCTGTCTTCTGACTTGGAGAAGCTGAGAGAAAACTTAGCAGATATGGAGGCAAAATTTAGA GAGAAAGATGAGAGAGAAGAGCAGCTGATAAAGGCAAAGGAAAAACTGGAAAATGACATTGCAGAAATAA TGAAGATGTCAGGAGATAACTCTTCTCAGCTGACAAAAATGAACGATGAATTACGTCTGAAAGAAAGAGA TGTAGAAGAATTACAGCTAAAACTTACAAAGGCTAATGAAAATGCAAGTTTTCTGCAAAAAAGTATTGAG GACATGACTGTCAAAGCTGAACAGAGCCAGCAAGAAGCAGCTAAAAAGCATGAGGAAGAAAAGAAAGAAT TGGAGAGGAAATTGTCGGACCTGGAAAAGAAAATGGAAACAAGCCACAACCAGTGTCAGGAGCTGAAAGC CAGGTATGAGAGAGCCACTTCTGAGACAAAAACCAAGCATGAAGAAATCCTACAGAACCTCCAGAAGACG CTGCTGGACACAGAGGACAAGCTGAAGGGCGCACGGGAGGAGAACAGTGGCTTGCTGCAGGAGCTGGAGG AGCTGAGAAAGCAAGCCGACAAAGCCAAAGCTGCTCAAACAGCGGAAGATGCCATGCAGATAATGGAACA GATGACCAAAGAGAAGACTGAGACTCTGGCCTCCTTGGAGGACACCAAGCAAACAAATGCAAAACTACAG AATGAATTGGACACACTTAAAGAAAACAACTTGAAAAATGTGGAAGAGCTGAACAAATCAAAAGAACTCC TGACTGTAGAGAATCAAAAAATGGAAGAATTTAGGAAAGAAATAGAAACCCTAAAGCAGGCAGCAGCTCA GAAGTCCCAGCAGCTTTCAGCGTTGCAAGAAGAGAACGTTAAACTTGCTGAGGAGCTGGGGAGAAGCAGG GACGAAGTCACAAGTCATCAAAAGCTGGAAGAAGAAAGATCTGTGCTCAATAATCAGTTGTTAGAAATGA AAAAAAGAGAATCCAAGTTCATAAAAGACGCAGATGAAGAGAAAGCTTCCTTGCAGAAATCCATCAGTAT AACTAGTGCCTTACTCACAGAAAAGGATGCCGAGCTGGAGAAACTGAGAAATGAGGTCACAGTGCTCAGG GGAGAAAACGCCTCTGCCAAGTCCTTGCATTCAGTTGTTCAGACTCTAGAGTCTGATAAGGTGAAGCTCG AGCTCAAGGTAAAGAACTTGGAGCTTCAACTCAAAGAAAACAAGAGGCAGCTCAGCAGCTCCTCAGGTAA TACAGACACTCAGGCAGACGAGGATGAAAGAGCCCAGGAGAGTCAGATTGATTTCCTAAATTCAGTAATA GTGGACCTTCAAAGGAAGAATCAAGACCTCAAGATGAAGGTGGAGATGATGTCAGAAGCAGCCCTGAATG GGAACGGGGATGACCTAAACAATTATGACAGTGATGATCAGGAGAAACAGTCCAAGAAGAAACCTCGCCT CTTCTGTGACATTTGTGACTGCTTTGATCTCCACGACACAGAGGATTGTCCTACCCAGGCACAGATGTCA GAGGACCCTCCCCATTCCACACACCATGGCAGTCGGGGTGAGGAACGCCCATACTGTGAAATCTGTGAGA TGTTTGGACACTGGGCCACCAACTGCAATGACGACGAAACCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235302 representing NM_001247997 Red=Cloning site Green=Tags(s) MSMLKPSGLKAPTKILKPGSTALKTPTAVVAPVEKTISSEKASSTPSSETQEEFVDDFRVGERVWVNGNK PGFIQFLGETQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQCEPLKGIFTRPSKLTRKVQAEDEANGLQTT PASRATSPLCTSTASMVSSSPSTPSNIPQKPSQPAAKEPSATPPISNLTKTASESISNLSEAGSIKKGER ELKIGDRVLVGGTKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVHKVTK IGFPSTTPAKAKANAVRRVMATTSASLKRSPSASSLSSMSSVASSVSSRPSRTGLLTETSSRYARKISGT TALQEALKEKQQHIEQLLAERDLERAEVAKATSHVGEIEQELALARDGHDQHVLELEAKMDQLRTMVEAA DREKVELLNQLEEEKRKVEDLQFRVEEESITKGDLEQKSQISEDPENTQTKLEHARIKELEQSLLFEKTK ADKLQRELEDTRVATVSEKSRIMELEKDLALRVQEVAELRRRLESNKPAGDVDMSLSLLQEISSLQEKLE VTRTDHQREITSLKEHFGAREETHQKEIKALYTATEKLSKENESLKSKLEHANKENSDVIALWKSKLETA IASHQQAMEELKVSFSKGLGTETAEFAELKTQIEKMRLDYQHEIENLQNQQDSERAAHAKEMEALRAKLM KVIKEKENSLEAIRSKLDKAEDQHLVEMEDTLNKLQEAEIKVKELEVLQAKCNEQTKVIDNFTSQLKATE EKLLDLDALRKASSEGKSEMKKLRQQLEAAEKQIKHLEIEKNAESSKASSITRELQGRELKLTNLQENLS EVSQVKETLEKELQILKEKFAEASEEAVSVQRSMQETVNKLHQKEEQFNMLSSDLEKLRENLADMEAKFR EKDEREEQLIKAKEKLENDIAEIMKMSGDNSSQLTKMNDELRLKERDVEELQLKLTKANENASFLQKSIE DMTVKAEQSQQEAAKKHEEEKKELERKLSDLEKKMETSHNQCQELKARYERATSETKTKHEEILQNLQKT LLDTEDKLKGAREENSGLLQELEELRKQADKAKAAQTAEDAMQIMEQMTKEKTETLASLEDTKQTNAKLQ NELDTLKENNLKNVEELNKSKELLTVENQKMEEFRKEIETLKQAAAQKSQQLSALQEENVKLAEELGRSR DEVTSHQKLEEERSVLNNQLLEMKKRESKFIKDADEEKASLQKSISITSALLTEKDAELEKLRNEVTVLR GENASAKSLHSVVQTLESDKVKLELKVKNLELQLKENKRQLSSSSGNTDTQADEDERAQESQIDFLNSVI VDLQRKNQDLKMKVEMMSEAALNGNGDDLNNYDSDDQEKQSKKKPRLFCDICDCFDLHDTEDCPTQAQMS EDPPHSTHHGSRGEERPYCEICEMFGHWATNCNDDETF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001247997
ORF Size	4314 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001247997.1, NP_001234926.1
RefSeq Size	5931 bp
RefSeq ORF	4317 bp
Locus ID	6249
UniProt ID	P30622
MW	162.7 kDa
Gene Summary	The protein encoded by this gene links endocytic vesicles to microtubules. This gene is highly expressed in Reed-Sternberg cells of Hodgkin disease. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

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