AATK (NM_004920) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AATYK; AATYK1; LMR1; LMTK1; p35BP; PPP1R77
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235251 representing NM_004920 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAGCAGCCTGGGCGCTCAGTGCAGCTCCTCAAGTCCACAGACGTGGGCCGGCACAGCCTCCTGT ACCTGAAGGAAATCGGCCGTGGCTGGTTCGGGAAGGTGTTCCTGGGGGAGGTGAACTCTGGCATCAGCAG TGCCCAGGTGGTGGTGAAGGAGCTGCAGGCTAGTGCCAGCGTGCAGGAGCAGATGCAGTTCCTGGAGGAG GTGCAGCCCTACAGGGCCCTGAAGCACAGCAACCTGCTCCAGTGCCTGGCCCAGTGCGCCGAGGTGACGC CCTACCTGCTGGTGATGGAGTTCTGCCCACTGGGGGACCTCAAGGGCTACCTGCGGAGCTGCCGGGTGGC GGAGTCCATGGCTCCCGACCCCCGGACCCTGCAGCGCATGGCCTGTGAGGTGGCCTGTGGCGTCCTGCAC CTTCATCGCAACAATTTCGTGCACAGCGACCTGGCCCTGCGGAACTGCCTGCTCACGGCTGACCTGACGG TGAAGATTGGTGACTATGGCCTGGCTCACTGCAAGTACAGAGAGGACTACTTCGTGACTGCCGACCAGCT GTGGGTGCCTCTGCGCTGGATCGCGCCAGAGCTGGTGGACGAGGTGCATAGCAACCTGCTCGTCGTGGAC CAGACCAAGAGCGGGAATGTGTGGTCCCTGGGCGTGACCATCTGGGAGCTCTTTGAGCTGGGCACGCAGC CCTATCCCCAGCACTCGGACCAGCAGGTGCTGGCGTACACGGTCCGGGAGCAGCAGCTCAAGCTGCCCAA GCCCCAGCTGCAGCTGACCCTGTCGGACCGCTGGTACGAGGTGATGCAGTTCTGCTGGCTGCAGCCCGAG CAGCGGCCCACAGCCGAGGAGGTGCACCTGCTGCTGTCCTACCTGTGTGCCAAGGGCGCCACCGAAGCAG AGGAGGAGTTTGAACGGCGCTGGCGCTCTCTGCGGCCCGGCGGGGGCGGCGTGGGGCCCGGGCCCGGTGC GGCGGGGCCCATGCTGGGCGGCGTGGTGGAGCTCGCCGCTGCCTCGTCCTTCCCGCTGCTGGAGCAGTTC GCGGGCGACGGCTTCCACGCGGACGGCGACGACGTGCTGACGGTGACCGAGACCAGCCGAGGCCTCAATT TTGAGTACAAGTGGGAGGCGGGCCGCGGCGCGGAGGCCTTCCCGGCCACGCTGAGCCCTGGCCGCACCGC ACGCCTGCAGGAGCTGTGCGCCCCCGACGGCGCGCCCCCGGGCGTGGTTCCGGTGCTCAGCGCGCACAGC CCGTCGCTGGGCAGCGAGTACTTCATCCGCCTAGAGGAGGCCGCACCCGCCGCCGGCCACGACCCTGACT GCGCCGGCTGCGCCCCCAGTCCACCTGCCACCGCGGACCAGGACGACGACTCTGACGGCAGCACCGCCGC CTCGCTGGCCATGGAGCCGCTGCTGGGCCACGGGCCACCCGTCGACGTCCCCTGGGGCCGCGGCGACCAC TACCCTCGCAGAAGCTTGGCGCGGGACCCGCTCTGCCCCTCACGCTCTCCCTCGCCCTCGGCGGGGCCCC TGAGTCTGGCGGAGGGAGGAGCGGAGGATGCAGACTGGGGCGTGGCCGCCTTCTGTCCTGCCTTCTTCGA GGACCCACTGGGCACGTCCCCTTTGGGGAGCTCAGGGGCGCCCCCGCTGCCGCTGACTGGCGAGGATGAG CTAGAGGAGGTGGGAGCGCGGAGGGCCGCCCAGCGCGGGCACTGGCGCTCCAACGTGTCAGCCAACAACA ACAGCGGCAGCCGCTGTCCAGAGTCCTGGGACCCCGTCTCTGCGGGCGGCCACGCTGAGGGCTGCCCCAG TCCAAAGCAGACCCCACGGGCCTCCCCCGAGCCGGGGTACCCTGGAGAGCCTCTGCTTGGGCTCCAGGCA GCCTCTGCCCAGGAGCCAGGCTGCTGCCCCGGCCTCCCTCATCTATGCTCTGCCCAGGGCCTGGCACCTG CTCCCTGCCTGGTTACACCCTCCTGGACAGAGACAGCCAGTAGTGGGGGTGACCACCCGCAGGCAGAGCC CAAGCTTGCCACGGAGGCTGAGGGCACTACCGGACCCCGCCTGCCCCTTCCTTCCGTCCCCTCCCCATCC CAGGAGGGAGCCCCACTTCCCTCGGAGGAGGCCAGTGCCCCCGACGCCCCTGATGCCCTGCCTGACTCTC CCACGCCTGCTACTGGTGGCGAGGTGTCTGCCATCAAGCTGGCTTCTGCCCTGAATGGCAGCAGCAGCTC TCCCGAGGTGGAGGCACCCAGCAGTGAGGATGAGGACACGGCCGAGGCCACCTCAGGCATCTTCACCGAC ACGTCCAGCGACGGCCTGCAGGCCAGGAGGCCGGATGTGGTGCCAGCCTTCCGCTCTCTGCAGAAGCAGG TGGGGACCCCCGACTCCCTGGACTCCCTGGACATCCCGTCCTCAGCCAGTGATGGTGGCTATGAGGTCTT CAGCCCGTCGGCCACTGGCCCCTCTGGAGGGCAGCCGCGAGCGCTGGACAGTGGCTATGACACCGAGAAC TATGAGTCCCCTGAGTTTGTGCTCAAGGAGGCGCAGGAAGGGTGTGAGCCCCAGGCCTTTGCGGAGCTGG CCTCAGAGGGTGAGGGCCCCGGGCCCGAGACACGGCTCTCCACCTCCCTCAGTGGCCTCAACGAGAAGAA TCCCTACCGAGACTCTGCCTACTTCTCAGACCTCGAGGCTGAGGCCGAGGCCACCTCAGGCCCAGAGAAG AAGTGCGGCGGGGACCGAGCCCCCGGGCCAGAGCTGGGCCTGCCGAGCACTGGGCAGCCGTCTGAGCAGG TCTGTCTCAGGCCTGGGGTTTCCGGGGAGGCACAAGGCTCTGGCCCCGGGGAGGTGCTGCCCCCACTGCT GCAGCTTGAAGGGTCCTCCCCAGAGCCCAGCACCTGCCCCTCGGGCCTGGTCCCAGAGCCTCCGGAGCCC CAAGGCCCAGCCAAGGTGCGGCCTGGGCCCAGCCCCAGCTGCTCCCAGTTTTTCCTGCTGACCCCGGTTC CGCTGAGATCAGAAGGCAACAGCTCTGAGTTCCAGGGGCCCCCAGGACTGTTGTCAGGGCCGGCCCCACA AAAGCGGATGGGGGGCCCAGGCACCCCCAGAGCCCCACTCCGCCTGGCTCTGCCCGGCCTCCCTGCGGCC TTGGAGGGCCGGCCGGAGGAGGAGGAGGAGGACAGTGAGGACAGCGACGAGTCTGACGAGGAGCTCCGCT GCTACAGCGTCCAGGAGCCTAGCGAGGACAGCGAAGAGGAGGCGCCGGCGGTGCCCGTGGTGGTGGCTGA GAGCCAGAGCGCGCGCAACCTGCGCAGCCTGCTCAAGATGCCCAGCCTGCTGTCCGAGACCTTCTGCGAG GACCTGGAACGCAAGAAGAAGGCCGTGTCCTTCTTCGACGACGTCACCGTCTACCTCTTTGACCAGGAAA GCCCCACCCGGGAGCTCGGGGAGCCCTTCCCGGGCGCCAAGGAATCGCCCCCTACGTTCCTTAGGGGGAG CCCCGGCTCTCCCAGCGCCCCCAACCGGCCGCAGCAGGCTGATGGCTCCCCAAATGGCTCCACAGCGGAA GAGGGTGGTGGGTTCGCGTGGGACGACGACTTCCCGCTGATGACGGCCAAGGCAGCCTTCGCCATGGCCC TAGACCCGGCCGCACCCGCCCCGGCTGCGCCCACGCCCACGCCCGCTCCCTTCTCGCGCTTCACGGTGTC GCCCGCGCCCACGTCCCGCTTCTCCATCACGCACGTGTCTGACTCGGACGCCGAGTCCAAGAGAGGACCT GAAGCTGGTGCCGGGGGTGAGAGTAAAGAGGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235251 representing NM_004920 Red=Cloning site Green=Tags(s) MAKQPGRSVQLLKSTDVGRHSLLYLKEIGRGWFGKVFLGEVNSGISSAQVVVKELQASASVQEQMQFLEE VQPYRALKHSNLLQCLAQCAEVTPYLLVMEFCPLGDLKGYLRSCRVAESMAPDPRTLQRMACEVACGVLH LHRNNFVHSDLALRNCLLTADLTVKIGDYGLAHCKYREDYFVTADQLWVPLRWIAPELVDEVHSNLLVVD QTKSGNVWSLGVTIWELFELGTQPYPQHSDQQVLAYTVREQQLKLPKPQLQLTLSDRWYEVMQFCWLQPE QRPTAEEVHLLLSYLCAKGATEAEEEFERRWRSLRPGGGGVGPGPGAAGPMLGGVVELAAASSFPLLEQF AGDGFHADGDDVLTVTETSRGLNFEYKWEAGRGAEAFPATLSPGRTARLQELCAPDGAPPGVVPVLSAHS PSLGSEYFIRLEEAAPAAGHDPDCAGCAPSPPATADQDDDSDGSTAASLAMEPLLGHGPPVDVPWGRGDH YPRRSLARDPLCPSRSPSPSAGPLSLAEGGAEDADWGVAAFCPAFFEDPLGTSPLGSSGAPPLPLTGEDE LEEVGARRAAQRGHWRSNVSANNNSGSRCPESWDPVSAGGHAEGCPSPKQTPRASPEPGYPGEPLLGLQA ASAQEPGCCPGLPHLCSAQGLAPAPCLVTPSWTETASSGGDHPQAEPKLATEAEGTTGPRLPLPSVPSPS QEGAPLPSEEASAPDAPDALPDSPTPATGGEVSAIKLASALNGSSSSPEVEAPSSEDEDTAEATSGIFTD TSSDGLQARRPDVVPAFRSLQKQVGTPDSLDSLDIPSSASDGGYEVFSPSATGPSGGQPRALDSGYDTEN YESPEFVLKEAQEGCEPQAFAELASEGEGPGPETRLSTSLSGLNEKNPYRDSAYFSDLEAEAEATSGPEK KCGGDRAPGPELGLPSTGQPSEQVCLRPGVSGEAQGSGPGEVLPPLLQLEGSSPEPSTCPSGLVPEPPEP QGPAKVRPGPSPSCSQFFLLTPVPLRSEGNSSEFQGPPGLLSGPAPQKRMGGPGTPRAPLRLALPGLPAA LEGRPEEEEEDSEDSDESDEELRCYSVQEPSEDSEEEAPAVPVVVAESQSARNLRSLLKMPSLLSETFCE DLERKKKAVSFFDDVTVYLFDQESPTRELGEPFPGAKESPPTFLRGSPGSPSAPNRPQQADGSPNGSTAE EGGGFAWDDDFPLMTAKAAFAMALDPAAPAPAAPTPTPAPFSRFTVSPAPTSRFSITHVSDSDAESKRGP EAGAGGESKEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_004920
ORF Size	3813 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_004920.2, NP_004911.2
RefSeq Size	5105 bp
RefSeq ORF	3816 bp
Locus ID	9625
UniProt ID	Q6ZMQ8
Protein Families	Druggable Genome, Protein Kinase
MW	134.3 kDa
Gene Summary	The protein encoded by this gene contains a tyrosine kinase domain at the N-terminus and a proline-rich domain at the C-terminus. This gene is induced during apoptosis, and expression of this gene may be a necessary pre-requisite for the induction of growth arrest and/or apoptosis of myeloid precursor cells. This gene has been shown to produce neuronal differentiation in a neuroblastoma cell line. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

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