RALGDS (NM_001271776) Human Tagged ORF Clone
CAT#: RC235009
- TrueORF®
RALGDS (Myc-DDK tagged) - Homo sapiens ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 5
ORF Plasmid: tGFP
"NM_001271776" in other vectors (2)
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CNY 7,130.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | RalGEF; RGDS; RGF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC235009 representing NM_001271776
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCAGCGCATGTGGGCCGAGGCGGCCGGGCCTGCTGGCGGCGCCGAGCCGCTGTTTCCGGGCTCCC GGCGGAGCCGCAGCGTGTGGGACGCCGTGCGCCTGGAGGTGGGCGTCCCCGACAGCTGCCCGGTGGTGCT GCACAGCTTCACGCAGCTAGACCCCGACCTGCCGCGCCCGGAGAGCTCCACGCAGGAGATCGGTGAGGAG CTGATCAACGGAGTCATCTACTCCATCTCCCTGCGCAAGGTGCAGCTGCACCACGGAGGCAACAAGGGGC AGCGCTGGCTCGGGTATGAGAATGAGTCGGCCCTGAACCTTTATGAGACTTGCAAGGTGCGGACCGTGAA GGCTGGCACGCTGGAGAAGCTGGTGGAGCACCTGGTGCCAGCCTTCCAGGGCAGCGACCTCTCCTACGTC ACCATCTTCCTGTGTACCTATAGAGCCTTCACCACCACCCAACAGGTCCTGGACCTGCTGTTCAAAAGAT ACGGCTGCATCCTCCCCTATTCCGACGAGGATGGTGGACCCCAGGACCAACTTAAAAATGCCATCTCCTC CATCCTGGGCACCTGGCTGGACCAGTACTCGGAGGATTTCTGTCAACCTCCGGACTTTCCCTGCCTCAAG CAGCTGGTGGCCTACGTGCAGCTCAACATGCCAGGCTCAGACCTGGAGCGCCGTGCCCACCTTCTCCTGG CCCAGCTGGAGCACTCGGAACCCATTGAGGCAGAGCCTGAGGCTCTGTCACCAGTGCCAGCTCTAAAACC AACTCCAGAGCTCGAGCTAGCTCTAACACCAGCTCGAGCACCCAGCCCAGTGCCGGCTCCAGCCCCGGAG CCAGAGCCAGCTCCAACACCAGCTCCAGGTTCAGAGCTAGAAGTAGCTCCAGCACCAGCTCCGGAGCTCC AGCAGGCTCCAGAGCCAGCTGTGGGACTAGAATCGGCTCCAGCGCCAGCTCTGGAACTAGAGCCAGCTCC AGAACAGGATCCAGCTCCCTCACAAACTCTAGAGCTGGAGCCAGCTCCAGCACCAGTTCCATCATTACAG CCTTCCTGGCCTTCACCTGTGGTTGCAGAGAACGGGCTGAGTGAGGAGAAGCCTCACCTCTTGGTGTTCC CTCCAGATCTGGTGGCAGAGCAGTTTACACTGATGGATGCGGAACTGTTCAAGAAGGTGGTGCCCTACCA CTGCCTGGGCTCCATCTGGTCCCAGCGGGACAAGAAGGGCAAGGAGCACCTGGCGCCCACCATCCGCGCC ACTGTCACCCAGTTCAACAGTGTGGCCAACTGTGTCATCACCACCTGCCTCGGGAACCGAAGCACGAAAG CCCCAGACAGGGCCAGGGTGGTGGAGCACTGGATCGAGGTGGCCAGGGAGTGCCGGATCCTCAAGAACTT CTCGTCACTGTATGCCATCCTCTCTGCCCTGCAGAGCAACTCCATCCACCGTCTGAAGAAGACGTGGGAA GACGTTTCCAGGGACAGTTTCCGGATCTTTCAGAAGCTGTCAGAGATCTTCTCAGATGAGAACAACTACT CATTGAGCCGGGAGCTGCTCATCAAGGAGGGCACCTCCAAGTTTGCCACCCTGGAGATGAACCCCAAGAG AGCCCAGAAACGGCCGAAGGAGACGGGCATCATCCAGGGCACCGTTCCCTACCTGGGCACGTTCCTCACC GACCTGGTGATGCTGGACACTGCCATGAAGGACTATCTGTATGGCAGACTCATCAACTTTGAGAAGAGGA GGAAGGAGTTCGAGGTGATCGCCCAGATCAAGCTGCTGCAGTCGGCCTGCAACAACTACAGCATCGCGCC AGATGAGCAATTTGGGGCCTGGTTCCGGGCCGTGGAGCGGCTCAGCGAGACTGAGAGCTACAACCTGTCG TGCGAGCTGGAGCCCCCATCCGAGTCAGCCAGCAACACCCTCAGGACCAAGAAGAACACAGCCATTGTCA AGCGCTGGAGCGACCGCCAGGCCCCCAGCACTGAGCTCAGTACCAGTGGCAGCTCCCACTCCAAGTCCTG TGACCAGCTCAGGTGTGGCCCCTACCTCAGCAGCGGGGACATCGCTGACGCGCTCAGCGTGCACTCGGCC GGCTCCTCTAGCTCCGACGTGGAGGAGATCAACATCAGCTTCGTCCCGGAGTCTCCTGATGGCCAGGAAA AGAAGTTCTGGGAATCAGCCTCACAGTCATCCCCGGAGACCTCCGGCATCAGCTCAGCCTCCAGCAGCAC CTCGTCCTCCTCAGCCTCCACCACGCCCGTGGCTGCCACACGCACCCACAAGCGCTCTGTCTCAGGGCTC TGCAACTCCAGCTCCGCGCTGCCGCTCTACAACCAGCAGGTGGGCGACTGCTGTATCATCCGCGTCAGCC TGGACGTGGACAATGGCAACATGTACAAGAGCATCCTGGTGACCAGCCAAGATAAGGCTCCGGCTGTAAT CCGCAAGGCCATGGACAAACACAACCTGGAGGAGGAGGAGCCGGAGGACTATGAGCTGCTGCAGATTCTC TCAGATGACCGGAAGCTGAAGATCCCTGAAAACGCCAACGTCTTCTATGCCATGAACTCTACCGCCAACT ATGACTTTGTCCTCAAGAAGCGGACCTTCACCAAGGGAGTGAAGGTCAAGCACGGAGCCAGCTCCACCCT CCCTCGCATGAAGCAGAAAGGACTCAAGATTGCCAAGGGCATCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC235009 representing NM_001271776
Red=Cloning site Green=Tags(s) MVQRMWAEAAGPAGGAEPLFPGSRRSRSVWDAVRLEVGVPDSCPVVLHSFTQLDPDLPRPESSTQEIGEE LINGVIYSISLRKVQLHHGGNKGQRWLGYENESALNLYETCKVRTVKAGTLEKLVEHLVPAFQGSDLSYV TIFLCTYRAFTTTQQVLDLLFKRYGCILPYSDEDGGPQDQLKNAISSILGTWLDQYSEDFCQPPDFPCLK QLVAYVQLNMPGSDLERRAHLLLAQLEHSEPIEAEPEALSPVPALKPTPELELALTPARAPSPVPAPAPE PEPAPTPAPGSELEVAPAPAPELQQAPEPAVGLESAPAPALELEPAPEQDPAPSQTLELEPAPAPVPSLQ PSWPSPVVAENGLSEEKPHLLVFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKEHLAPTIRA TVTQFNSVANCVITTCLGNRSTKAPDRARVVEHWIEVARECRILKNFSSLYAILSALQSNSIHRLKKTWE DVSRDSFRIFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEMNPKRAQKRPKETGIIQGTVPYLGTFLT DLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNYSIAPDEQFGAWFRAVERLSETESYNLS CELEPPSESASNTLRTKKNTAIVKRWSDRQAPSTELSTSGSSHSKSCDQLRCGPYLSSGDIADALSVHSA GSSSSDVEEINISFVPESPDGQEKKFWESASQSSPETSGISSASSSTSSSSASTTPVAATRTHKRSVSGL CNSSSALPLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNLEEEEPEDYELLQIL SDDRKLKIPENANVFYAMNSTANYDFVLKKRTFTKGVKVKHGASSTLPRMKQKGLKIAKGIF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001271776 |
ORF Size | 2706 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001271776.2 |
RefSeq Size | 3694 bp |
RefSeq ORF | 2709 bp |
Locus ID | 5900 |
UniProt ID | Q12967 |
Protein Pathways | Colorectal cancer, Pancreatic cancer, Pathways in cancer |
MW | 99.8 kDa |
Gene Summary | Guanine nucleotide dissociation stimulators (GDSs, or exchange factors), such as RALGDS, are effectors of Ras-related GTPases (see MIM 190020) that participate in signaling for a variety of cellular processes.[supplied by OMIM, Nov 2010] |
Documents
Product Manuals |
FAQs |
SDS |