NALP6 (NLRP6) (NM_001276700) Human Tagged ORF Clone
CAT#: RC234995
- TrueORF®
NLRP6 (Myc-DDK tagged) - Homo sapiens NLR family, pyrin domain containing 6 (NLRP6), transcript variant 2
ORF Plasmid: tGFP
"NM_001276700" in other vectors (2)
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CNY 7,130.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AVR; CLR11.4; NALP6; NAVR; NAVR/AVR; PAN3; PYPAF5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC234995 representing NM_001276700
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCAGCCAGAGGCCCCCTGCTCCAGCACGGGGCCGCGCCTCGCGGTGGCCCGCGAGCTGCTCCTGG CTGCGCTGGAGGAACTGAGCCAAGAGCAGCTGAAGCGCTTCCGCCACAAGCTGCGCGACGTGGGCCCGGA CGGACGCAGCATCCCGTGGGGGCGGCTGGAGCGCGCGGACGCCGTGGACCTCGCGGAGCAGCTGGCCCAG TTCTACGGCCCGGAGCCTGCCCTGGAGGTGGCCCGCAAGACCCTCAAGAGGGCGGACGCGCGCGACGTGG CGGCGCAGCTCCAGGAGCGGCGGCTGCAGCGGCTCGGGCTCGGCTCCGGGACGCTGCTCTCCGTGTCCGA GTACAAGAAGAAGTACCGGGAGCACGTGCTGCAGCTGCACGCTCGGGTGAAGGAGAGGAACGCCCGCTCC GTGAAGATCACCAAGCGCTTCACCAAGCTGCTCATCGCGCCCGAGAGCGCCGCCCCGGAGGAGGCGATGG GGCCCGCGGAAGAGCCTGAGCCGGGGCGCGCGCGGCGCTCGGACACGCACACTTTCAACCGCCTCTTCCG CCGCGACGAGGAGGGCCGGCGGCCGCTGACCGTGGTGCTGCAGGGCCCGGCGGGCATCGGCAAGACCATG GCGGCCAAAAAGATCCTGTACGACTGGGCGGCGGGCAAGCTGTACCAGGGCCAGGTGGACTTCGCCTTCT TCATGCCCTGCGGCGAGCTGCTGGAGAGGCCGGGCACGCGCAGCCTGGCTGACCTGATCCTGGACCAGTG CCCCGACCGCGGCGCGCCGGTGCCGCAGATGCTGGCCCAGCCGCAGCGGCTGCTCTTCATCCTGGACGGC GCGGACGAGCTGCCGGCGCTGGGGGGCCCCGAGGCCGCGCCCTGCACAGACCCCTTCGAGGCGGCGAGCG GCGCGCGGGTGCTAGGCGGGCTGCTGAGCAAGGCGCTGCTGCCCACGGCCCTCCTGCTGGTGACCACGCG CGCCGCCGCCCCCGGGAGGCTGCAGGGCCGCCTGTGTTCCCCGCAGTGCGCCGAGGTGCGCGGCTTCTCC GACAAGGACAAGAAGAAGTATTTCTACAAGTATTTCCGGGATGAGAGGAGGGCCGAGCGCGCCTACCGCT TCGTGAAGGAGAACGAGACGCTGTTCGCGCTGTGCTTCGTGCCCTTCGTGTGCTGGATCGTGTGCACCGT GCTGCGCCAGCAGCTGGAGCTCGGTCGGGACCTGTCGCGCACGTCCAAGACCACCACGTCAGTGTACCTG CTTTTCATCACCAGCGTTCTGAGCTCGGCTCCGGTAGCCGACGGGCCCCGGTTGCAGGGCGACCTGCGCA ATCTGTGCCGCCTGGCCCGCGAGGGCGTCCTCGGACGCAGGGCGCAGTTTGCCGAGAAGGAACTGGAGCA ACTGGAGCTTCGTGGCTCCAAAGTGCAGACGCTGTTTCTCAGCAAAAAGGAGCTGCCGGGCGTGCTGGAG ACAGAGGTCACCTACCAGTTCATCGACCAGAGCTTCCAGGAGTTCCTCGCGGCACTGTCCTACCTGCTGG AGGACGGCGGGGTGCCCAGGACCGCGGCTGGCGGCGTTGGGACACTCCTGCGTGGGGACGCCCAGCCGCA CAGCCACTTGGTGCTCACCACGCGCTTCCTCTTCGGACTGCTGAGCGCGGAGCGGATGCGCGACATCGAG CGCCACTTCGGCTGCATGGTTTCAGAGCGTGTGAAGCAGGAGGCCCTGCGGTGGGTGCAGGGACAGGGAC AGGGCTGCCCCGGAGTGGCACCAGAGGTGACCGAGGGGGCCAAAGGGCTCGAGGACACCGAAGAGCCAGA GGAGGAGGAGGAGGGAGAGGAGCCCAACTACCCACTGGAGTTGCTGTACTGCCTGTACGAGACGCAGGAG GACGCGTTTGTGCGCCAAGCCCTGTGCCGGTTCCCGGAGCTGGCGCTGCAGCGAGTGCGCTTCTGCCGCA TGGACGTGGCTGTTCTGAGCTACTGCGTGAGGTGCTGCCCTGCTGGACAGGCACTGCGGCTGATCAGCTG CAGATTGGTTGCTGCGCAGGAGAAGAAGAAGAAGAGCCTGGGGAAGCGGCTCCAGGCCAGCCTGGGTGGC GGCAGTTCTCAAGGCACCACAAAACAACTGCCAGCCTCCCTTCTTCATCCACTCTTTCAGGCAATGACTG ACCCACTGTGCCATCTGAGCAGCCTCACGCTGTCCCACTGCAAACTCCCTGACGCGGTCTGCCGAGACCT TTCTGAGGCCCTGAGGGCAGCCCCCGCACTGACGGAGCTGGGCCTCCTCCACAACAGGCTCAGTGAGGCG GGACTGCGTATGCTGAGTGAGGGCCTAGCCTGGCCGCAGTGCAGGGTGCAGACGGTCAGGGTACAGCTGC CTGACCCCCAGCGAGGGCTCCAGTACCTGGTGGGTATGCTTCGGCAGAGCCCTGCCCTGACCACCCTGGA TCTCAGCGGCTGCCAACTGCCCGCCCCCATGGTGACCTACCTGTGTGCAGTCCTGCAGCACCAGGGATGC GGCCTGCAGACCCTCAGTCTGGCCTCTGTGGAGCTGAGCGAGCAGTCACTACAGGAGCTTCAGGCTGTGA AGAGAGCAAAGCCGGATCTGGTCATCACACACCCAGCGCTGGACGGCCACCCACAACCTCCCAAGGAACT CATCTCGACCTTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC234995 representing NM_001276700
Red=Cloning site Green=Tags(s) MDQPEAPCSSTGPRLAVARELLLAALEELSQEQLKRFRHKLRDVGPDGRSIPWGRLERADAVDLAEQLAQ FYGPEPALEVARKTLKRADARDVAAQLQERRLQRLGLGSGTLLSVSEYKKKYREHVLQLHARVKERNARS VKITKRFTKLLIAPESAAPEEAMGPAEEPEPGRARRSDTHTFNRLFRRDEEGRRPLTVVLQGPAGIGKTM AAKKILYDWAAGKLYQGQVDFAFFMPCGELLERPGTRSLADLILDQCPDRGAPVPQMLAQPQRLLFILDG ADELPALGGPEAAPCTDPFEAASGARVLGGLLSKALLPTALLLVTTRAAAPGRLQGRLCSPQCAEVRGFS DKDKKKYFYKYFRDERRAERAYRFVKENETLFALCFVPFVCWIVCTVLRQQLELGRDLSRTSKTTTSVYL LFITSVLSSAPVADGPRLQGDLRNLCRLAREGVLGRRAQFAEKELEQLELRGSKVQTLFLSKKELPGVLE TEVTYQFIDQSFQEFLAALSYLLEDGGVPRTAAGGVGTLLRGDAQPHSHLVLTTRFLFGLLSAERMRDIE RHFGCMVSERVKQEALRWVQGQGQGCPGVAPEVTEGAKGLEDTEEPEEEEEGEEPNYPLELLYCLYETQE DAFVRQALCRFPELALQRVRFCRMDVAVLSYCVRCCPAGQALRLISCRLVAAQEKKKKSLGKRLQASLGG GSSQGTTKQLPASLLHPLFQAMTDPLCHLSSLTLSHCKLPDAVCRDLSEALRAAPALTELGLLHNRLSEA GLRMLSEGLAWPQCRVQTVRVQLPDPQRGLQYLVGMLRQSPALTTLDLSGCQLPAPMVTYLCAVLQHQGC GLQTLSLASVELSEQSLQELQAVKRAKPDLVITHPALDGHPQPPKELISTF SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001276700 |
ORF Size | 2673 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001276700.2 |
RefSeq Size | 2760 bp |
RefSeq ORF | 2676 bp |
Locus ID | 171389 |
UniProt ID | P59044 |
MW | 99.1 kDa |
Gene Summary | The protein encoded by this gene binds arginine-vasopressin and may be involved in the arginine-vasopressin-mediated regulation of renal salt-water balance. The encoded protein also mediates inflammatory responses in the colon to allow recovery from intestinal epithelial damage and protects against tumorigenesis and the development of colitis. Finally, this protein can increase activation of NF-kappa-B, activation of CASP1 through interaction with ASC, and cAMP accumulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013] |
Documents
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SDS |