NALP6 (NLRP6) (NM_001276700) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AVR; CLR11.4; NALP6; NAVR; NAVR/AVR; PAN3; PYPAF5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234995 representing NM_001276700 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCAGCCAGAGGCCCCCTGCTCCAGCACGGGGCCGCGCCTCGCGGTGGCCCGCGAGCTGCTCCTGG CTGCGCTGGAGGAACTGAGCCAAGAGCAGCTGAAGCGCTTCCGCCACAAGCTGCGCGACGTGGGCCCGGA CGGACGCAGCATCCCGTGGGGGCGGCTGGAGCGCGCGGACGCCGTGGACCTCGCGGAGCAGCTGGCCCAG TTCTACGGCCCGGAGCCTGCCCTGGAGGTGGCCCGCAAGACCCTCAAGAGGGCGGACGCGCGCGACGTGG CGGCGCAGCTCCAGGAGCGGCGGCTGCAGCGGCTCGGGCTCGGCTCCGGGACGCTGCTCTCCGTGTCCGA GTACAAGAAGAAGTACCGGGAGCACGTGCTGCAGCTGCACGCTCGGGTGAAGGAGAGGAACGCCCGCTCC GTGAAGATCACCAAGCGCTTCACCAAGCTGCTCATCGCGCCCGAGAGCGCCGCCCCGGAGGAGGCGATGG GGCCCGCGGAAGAGCCTGAGCCGGGGCGCGCGCGGCGCTCGGACACGCACACTTTCAACCGCCTCTTCCG CCGCGACGAGGAGGGCCGGCGGCCGCTGACCGTGGTGCTGCAGGGCCCGGCGGGCATCGGCAAGACCATG GCGGCCAAAAAGATCCTGTACGACTGGGCGGCGGGCAAGCTGTACCAGGGCCAGGTGGACTTCGCCTTCT TCATGCCCTGCGGCGAGCTGCTGGAGAGGCCGGGCACGCGCAGCCTGGCTGACCTGATCCTGGACCAGTG CCCCGACCGCGGCGCGCCGGTGCCGCAGATGCTGGCCCAGCCGCAGCGGCTGCTCTTCATCCTGGACGGC GCGGACGAGCTGCCGGCGCTGGGGGGCCCCGAGGCCGCGCCCTGCACAGACCCCTTCGAGGCGGCGAGCG GCGCGCGGGTGCTAGGCGGGCTGCTGAGCAAGGCGCTGCTGCCCACGGCCCTCCTGCTGGTGACCACGCG CGCCGCCGCCCCCGGGAGGCTGCAGGGCCGCCTGTGTTCCCCGCAGTGCGCCGAGGTGCGCGGCTTCTCC GACAAGGACAAGAAGAAGTATTTCTACAAGTATTTCCGGGATGAGAGGAGGGCCGAGCGCGCCTACCGCT TCGTGAAGGAGAACGAGACGCTGTTCGCGCTGTGCTTCGTGCCCTTCGTGTGCTGGATCGTGTGCACCGT GCTGCGCCAGCAGCTGGAGCTCGGTCGGGACCTGTCGCGCACGTCCAAGACCACCACGTCAGTGTACCTG CTTTTCATCACCAGCGTTCTGAGCTCGGCTCCGGTAGCCGACGGGCCCCGGTTGCAGGGCGACCTGCGCA ATCTGTGCCGCCTGGCCCGCGAGGGCGTCCTCGGACGCAGGGCGCAGTTTGCCGAGAAGGAACTGGAGCA ACTGGAGCTTCGTGGCTCCAAAGTGCAGACGCTGTTTCTCAGCAAAAAGGAGCTGCCGGGCGTGCTGGAG ACAGAGGTCACCTACCAGTTCATCGACCAGAGCTTCCAGGAGTTCCTCGCGGCACTGTCCTACCTGCTGG AGGACGGCGGGGTGCCCAGGACCGCGGCTGGCGGCGTTGGGACACTCCTGCGTGGGGACGCCCAGCCGCA CAGCCACTTGGTGCTCACCACGCGCTTCCTCTTCGGACTGCTGAGCGCGGAGCGGATGCGCGACATCGAG CGCCACTTCGGCTGCATGGTTTCAGAGCGTGTGAAGCAGGAGGCCCTGCGGTGGGTGCAGGGACAGGGAC AGGGCTGCCCCGGAGTGGCACCAGAGGTGACCGAGGGGGCCAAAGGGCTCGAGGACACCGAAGAGCCAGA GGAGGAGGAGGAGGGAGAGGAGCCCAACTACCCACTGGAGTTGCTGTACTGCCTGTACGAGACGCAGGAG GACGCGTTTGTGCGCCAAGCCCTGTGCCGGTTCCCGGAGCTGGCGCTGCAGCGAGTGCGCTTCTGCCGCA TGGACGTGGCTGTTCTGAGCTACTGCGTGAGGTGCTGCCCTGCTGGACAGGCACTGCGGCTGATCAGCTG CAGATTGGTTGCTGCGCAGGAGAAGAAGAAGAAGAGCCTGGGGAAGCGGCTCCAGGCCAGCCTGGGTGGC GGCAGTTCTCAAGGCACCACAAAACAACTGCCAGCCTCCCTTCTTCATCCACTCTTTCAGGCAATGACTG ACCCACTGTGCCATCTGAGCAGCCTCACGCTGTCCCACTGCAAACTCCCTGACGCGGTCTGCCGAGACCT TTCTGAGGCCCTGAGGGCAGCCCCCGCACTGACGGAGCTGGGCCTCCTCCACAACAGGCTCAGTGAGGCG GGACTGCGTATGCTGAGTGAGGGCCTAGCCTGGCCGCAGTGCAGGGTGCAGACGGTCAGGGTACAGCTGC CTGACCCCCAGCGAGGGCTCCAGTACCTGGTGGGTATGCTTCGGCAGAGCCCTGCCCTGACCACCCTGGA TCTCAGCGGCTGCCAACTGCCCGCCCCCATGGTGACCTACCTGTGTGCAGTCCTGCAGCACCAGGGATGC GGCCTGCAGACCCTCAGTCTGGCCTCTGTGGAGCTGAGCGAGCAGTCACTACAGGAGCTTCAGGCTGTGA AGAGAGCAAAGCCGGATCTGGTCATCACACACCCAGCGCTGGACGGCCACCCACAACCTCCCAAGGAACT CATCTCGACCTTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234995 representing NM_001276700 Red=Cloning site Green=Tags(s) MDQPEAPCSSTGPRLAVARELLLAALEELSQEQLKRFRHKLRDVGPDGRSIPWGRLERADAVDLAEQLAQ FYGPEPALEVARKTLKRADARDVAAQLQERRLQRLGLGSGTLLSVSEYKKKYREHVLQLHARVKERNARS VKITKRFTKLLIAPESAAPEEAMGPAEEPEPGRARRSDTHTFNRLFRRDEEGRRPLTVVLQGPAGIGKTM AAKKILYDWAAGKLYQGQVDFAFFMPCGELLERPGTRSLADLILDQCPDRGAPVPQMLAQPQRLLFILDG ADELPALGGPEAAPCTDPFEAASGARVLGGLLSKALLPTALLLVTTRAAAPGRLQGRLCSPQCAEVRGFS DKDKKKYFYKYFRDERRAERAYRFVKENETLFALCFVPFVCWIVCTVLRQQLELGRDLSRTSKTTTSVYL LFITSVLSSAPVADGPRLQGDLRNLCRLAREGVLGRRAQFAEKELEQLELRGSKVQTLFLSKKELPGVLE TEVTYQFIDQSFQEFLAALSYLLEDGGVPRTAAGGVGTLLRGDAQPHSHLVLTTRFLFGLLSAERMRDIE RHFGCMVSERVKQEALRWVQGQGQGCPGVAPEVTEGAKGLEDTEEPEEEEEGEEPNYPLELLYCLYETQE DAFVRQALCRFPELALQRVRFCRMDVAVLSYCVRCCPAGQALRLISCRLVAAQEKKKKSLGKRLQASLGG GSSQGTTKQLPASLLHPLFQAMTDPLCHLSSLTLSHCKLPDAVCRDLSEALRAAPALTELGLLHNRLSEA GLRMLSEGLAWPQCRVQTVRVQLPDPQRGLQYLVGMLRQSPALTTLDLSGCQLPAPMVTYLCAVLQHQGC GLQTLSLASVELSEQSLQELQAVKRAKPDLVITHPALDGHPQPPKELISTF SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276700
ORF Size	2673 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276700.2
RefSeq Size	2760 bp
RefSeq ORF	2676 bp
Locus ID	171389
UniProt ID	P59044
MW	99.1 kDa
Gene Summary	The protein encoded by this gene binds arginine-vasopressin and may be involved in the arginine-vasopressin-mediated regulation of renal salt-water balance. The encoded protein also mediates inflammatory responses in the colon to allow recovery from intestinal epithelial damage and protects against tumorigenesis and the development of colitis. Finally, this protein can increase activation of NF-kappa-B, activation of CASP1 through interaction with ASC, and cAMP accumulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]

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