CARD14 (NM_001257970) Human Tagged ORF Clone
CAT#: RC234804
- TrueORF®
CARD14 (Myc-DDK tagged) - Homo sapiens caspase recruitment domain family, member 14 (CARD14), transcript variant 3
ORF Plasmid: tGFP
"NM_001257970" in other vectors (2)
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CNY 8,128.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BIMP2; CARMA2; PRP; PSORS2; PSS1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC234804 representing NM_001257970
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGAACTGTGCCGCAGGGACTCCGCACTCACGGCACTGGACGAGGAGACACTGTGGGAGATGATGG AGAGCCACCGCCACAGGATCGTACGCTGCATCTGCCCCAGCCGCCTCACCCCCTACCTGCGCCAGGCCAA GGTGCTGTGCCAGCTGGACGAGGAGGAGGTGCTGCACAGCCCCCGGCTCACCAACAGCGCCATGCGGGCC GGGCACTTGCTGGATTTGCTGAAGACTCGAGGGAAGAACGGGGCCATCGCCTTCCTGGAGAGCCTGAAGT TCCACAACCCTGACGTCTACACCCTGGTCACCGGGCTGCAGCCTGATGTTGACTTCAGTAACTTTAGCGG TCTCATGGAGACATCCAAGCTGACCGAGTGCCTGGCTGGGGCCATCGGCAGCCTGCAGGAGGAGCTGAAC CAGGAAAAGGGGCAGAAGGAGGTGCTGCTGCGGCGGTGCCAGCAGCTGCAGGAGCACCTGGGCCTGGCCG AGACCCGTGCCGAGGGCCTGCACCAGCTGGAGGCTGACCACAGCCGCATGAAGCGTGAGGTTAGCGCACA CTTCCATGAGGTGCTGAGGCTGAAGGACGAGATGCTCAGCCTCTCGCTGCACTATAGCAATGCGCTGCAG GAGAAGGAGCTGGCCGCCTCACGCTGCCGCAGCCTGCAGGAGGAGCTGTATCTACTGAAGCAGGAGCTGC AGCGAGCCAACATGGTTTCCTCCTGTGAGCTGGAATTGCAAGAGCAGTCCCTGAGGACAGCCAGCGACCA GGAGTCCGGGGATGAGGAGCTGAACCGCCTGAAGGAGGAGAATGAGAAACTGCGCTCGCTGACTTTCAGC CTGGCGGAGAAGGACATTCTGGAGCAGAGCCTGGACGAGGCGCGGGGGAGCCGACAGGAGCTGGTGGAGC GCATCCACTCGCTGCGGGAGCGGGCCGTGGCTGCCGAGAGGCAGCGAGAGCAGTACTGGGAAGAGAAGGA ACAGACCCTGCTGCAGTTCCAGAAGAGTAAGATGGCCTGCCAACTCTACAGGGAGAAGGTGAATGCGCTG CAGGCCCAGGTGTGCGAGCTGCAGAAGGAGCGAGACCAGGCGTACTCCGCGAGGGACAGTGCTCAGAGGG AGATTTCCCAGAGCCTGGTGGAGAAGGACTCCCTCCGCAGGCAGGTGTTCGAGCTGACGGACCAGGTCTG CGAGCTGCGCACACAGCTTCGCCAGCTGCAGGCAGAGCCTCCGGGTGTGCTCAAGCAGGAAGCCAGGACC AGGGAGCCCTGTCCACGGGAGAAGCAGCGGCTGGTGCGGATGCATGCCATCTGCCCCAGAGACGACAGCG ACTGCAGCCTCGTCAGCTCCACAGAGTCTCAGCTCTTGTCGGACCTGAGTGCCACGTCCAGCCGCGAGCT GGTGGACAGCTTCCGCTCCAGCAGCCCCGCGCCCCCCAGCCAGCAGTCCCTGTACAAGCGGGTGGCCGAG GACTTCGGGGAAGAACCCTGGTCTTTCAGCAGCTGCCTGGAGATCCCGGAGGGAGACCCGGGAGCCCTGC CGGGAGCTAAGGCAGGCGACCCACACCTGGATTATGAGCTCCTAGACACGGCAGACCTTCCGCAGCTGGA AAGCAGCCTGCAGCCAGTCTCCCCTGGAAGGCTTGATGTCTCGGAGAGCGGCGTCCTCATGCGGCGGAGG CCAGCCCGCAGGATCCTGAGCCAGGTCACCATGCTGGCGTTCCAGGGGGATGCATTGCTGGAGCAGATCA GCGTCATCGGCGGGAACCTCACGGGCATCTTCATCCACCGGGTCACCCCGGGCTCGGCGGCGGACCAGAT GGCCTTGCGCCCGGGCACCCAGATTGTGATGGTTGATTACGAAGCCTCAGAGCCCTTGTTCAAGGCAGTC CTGGAGGACACGACCCTGGAGGAGGCCGTGGGGCTTCTCAGGAGGGTGGACGGCTTCTGCTGCCTGTCTG TGAAGGTCAACACGGACGGTTATAAGAGGCTACTCCAGGACCTGGAGGCCAAAGTGGCGACCTCGGGGGA CTCATTCTACATCCGGGTCAACCTGGCCATGGAGGGCAGGGCCAAAGGGGAGCTGCAGGTGCATTGCAAC GAGGTCCTGCACGTCACCGACACCATGTTCCAGGGCTGCGGCTGCTGGCATGCCCACCGCGTGAACTCTT ACACCATGAAGGATACTGCCGCGCACGGCACCATCCCCAACTACTCCAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC234804 representing NM_001257970
Red=Cloning site Green=Tags(s) MGELCRRDSALTALDEETLWEMMESHRHRIVRCICPSRLTPYLRQAKVLCQLDEEEVLHSPRLTNSAMRA GHLLDLLKTRGKNGAIAFLESLKFHNPDVYTLVTGLQPDVDFSNFSGLMETSKLTECLAGAIGSLQEELN QEKGQKEVLLRRCQQLQEHLGLAETRAEGLHQLEADHSRMKREVSAHFHEVLRLKDEMLSLSLHYSNALQ EKELAASRCRSLQEELYLLKQELQRANMVSSCELELQEQSLRTASDQESGDEELNRLKEENEKLRSLTFS LAEKDILEQSLDEARGSRQELVERIHSLRERAVAAERQREQYWEEKEQTLLQFQKSKMACQLYREKVNAL QAQVCELQKERDQAYSARDSAQREISQSLVEKDSLRRQVFELTDQVCELRTQLRQLQAEPPGVLKQEART REPCPREKQRLVRMHAICPRDDSDCSLVSSTESQLLSDLSATSSRELVDSFRSSSPAPPSQQSLYKRVAE DFGEEPWSFSSCLEIPEGDPGALPGAKAGDPHLDYELLDTADLPQLESSLQPVSPGRLDVSESGVLMRRR PARRILSQVTMLAFQGDALLEQISVIGGNLTGIFIHRVTPGSAADQMALRPGTQIVMVDYEASEPLFKAV LEDTTLEEAVGLLRRVDGFCCLSVKVNTDGYKRLLQDLEAKVATSGDSFYIRVNLAMEGRAKGELQVHCN EVLHVTDTMFQGCGCWHAHRVNSYTMKDTAAHGTIPNYSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001257970 |
ORF Size | 2220 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001257970.1, NP_001244899.1 |
RefSeq Size | 2612 bp |
RefSeq ORF | 2223 bp |
Locus ID | 79092 |
UniProt ID | Q9BXL6 |
Protein Families | Druggable Genome |
MW | 83.7 kDa |
Gene Summary | This gene encodes a caspase recruitment domain-containing protein that is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. Members of this protein family are scaffold proteins that are involved in a diverse array of cellular processes including cellular adhesion, signal transduction and cell polarity control. This protein has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012] |
Documents
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FAQs |
SDS |