ANKRD6 (NM_001242809) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234782 representing NM_001242809 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCAGCAAGATGCGGTCGCTGCACTTTCAGAGCGCCTTCTCGTAGCTGCGTACAAAGGCCAAACAG AGAATGTGGTTCAGCTCATCAACAAGGGCGCCAGGGTAGCGGTTACCAAGCATGGCCGGACTCCCCTGCA TCTTGCTGCCAATAAGGGCCATCTTCCTGTGGTCCAGATCTTGCTGAAGGCTGGCTGCGACCTTGATGTC CAGGATGATGGGGACCAGACCGCCTTGCACCGGGCCACAGTGGTGGGGAACACGGAGATCATCGCGGCGC TCATCCACGAAGGGTGTGCCCTGGACAGACAAGACAAGGATGGGAATACAGCCTTGCATGAAGCATCCTG GCATGGTTTCAGCCAGTCAGCCAAGCTGCTCATTAAAGCAGGAGCCAACGTGCTTGCCAAGAACAAGGCG GGGAACACAGCTCTGCACCTGGCCTGCCAGAACAGCCACTCCCAGAGCACGCGCGTCCTCCTGCTGGCCG GGTCCCGCGCTGACCTCAAAAATAATGCAGGAGACACCTGTTTGCACGTTGCTGCGCGCTATAATCACTT GTCCATCATTAGGCTCCTCCTCACTGCTTTCTGTTCTGTCCATGAAAAGAACCAGGCTGGAGACACAGCA CTTCACGTTGCTGCTGCCCTAAATCACAAGAAGGTGGCCAAAATCTTACTGGAAGCCGGAGCAGATACGA CCATTGTTAACAATGCAGGCCAGACTCCGCTGGAGACTGCCCGCTACCACAATAACCCGGAAGTTGCTCT TCTCCTTACTAAAGCTCCCCAGGTCTTGCGCTTCAGTCGTGGGCGAAGCCTGAGGAAAAAGAGAGAGAGG CTCAAGGAAGAGAGGAGAGCCCAGTCTGTGCCAAGAGATGAGGTGGCCCAAAGCAAGGGCAGTGTCTCAG CAGGAGACACCCCCAGCAGTGAACAGGCTGTGGCCAGAAAAGAAGAAGCCAGAGAAGAGTTCCTGTCAGC CTCCCCAGAACCCAGAGCAAAGGATGACAGGAGGAGAAAGTCAAGGCCCAAGGTGTCAGCATTTTCTGAC CCCACCCCACCAGCCGACCAACAGCCTGGACACCAGAAGAACCTGCATGCTCATAATCACCCTAAAAAGA GGAACAGGCATCGGTGTTCATCCCCACCCCCACCCCATGAGTTCAGGGCGTATCAGCTCTACACATTGTA CCGGGGCAAGGATGGGAAAGTGATGCAGGCACCAATAAATGGTTGTCGATGTGAACCTCTAATCAACAAG CTGGAGAATCAGTTGGAGGCTACTGTGGAGGAGATAAAAGCAGAGCTGGGATCGGTTCAGGACAAAATGA ATACAAAGCTGGGGCAGATGGAGAATAAGACCCAGCACCAAATGCGTGTTTTGGACAAGCTGATGGTTGA GCGACTTTCTGCAGAGAGGACGGAGTGCCTGAACCGCCTGCAACAGCACTCAGACACAGAGAAGCATGAG GGGGAGAAACGACAGATATCCTTGGTGGATGAATTAAAAACCTGGTGCATGTTAAAGATTCAGAATCTGG AGCAGAAGCTTTCTGGAGATTCTAGGGCCTGCAGAGCTAAATCCACACCATCTACTTGTGAGTCCTCTAC AGGTGTGGACCAATTAGTGGTGACTGCAGGTCCAGCAGCAGCTTCCGACAGCTCCCCTCCAGTGGTTAGG CCCAAAGAGAAGGCCCTCAACTCCACTGCTACCCAGAGACTCCAGCAGGAGCTGTCGTCTTCTGACTGTA CAGGCTCCCGACTGAGAAACGTCAAGGTCCAGACAGCCTTGCTACCCATGAATGAGGCAGCCAGATCTGA TCAGCAGGCTGGGCCCTGCGTCAACAGAGGCACTCAAACTAAGAAGTCTGGGAAGAGTGGGCCAACAAGG CATCGTGCCCAGCAACCCGCAGCCAGCAGCACCTGTGGGCAGCCGCCACCAGCCACAGGCAGCGAGCAGA CTGGCCCTCACATTCGGGACACCTCCCAAGCTCTGGAGCTTACCCAGTATTTTTTTGAGGCTGTTTCTAC CCAGATGGAAAAGTGGTATGAAAGGAAGATTGAAGAAGCACGAAGCCAAGCCAATCAGAAAGCCCAGCAA GATAAGGCTACATTGAAGGAACACATTAAAAGTTTAGAAGAGGAACTTGCCAAACTAAGGACTAGGGTGC AGAAGGAAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234782 representing NM_001242809 Red=Cloning site Green=Tags(s) MSQQDAVAALSERLLVAAYKGQTENVVQLINKGARVAVTKHGRTPLHLAANKGHLPVVQILLKAGCDLDV QDDGDQTALHRATVVGNTEIIAALIHEGCALDRQDKDGNTALHEASWHGFSQSAKLLIKAGANVLAKNKA GNTALHLACQNSHSQSTRVLLLAGSRADLKNNAGDTCLHVAARYNHLSIIRLLLTAFCSVHEKNQAGDTA LHVAAALNHKKVAKILLEAGADTTIVNNAGQTPLETARYHNNPEVALLLTKAPQVLRFSRGRSLRKKRER LKEERRAQSVPRDEVAQSKGSVSAGDTPSSEQAVARKEEAREEFLSASPEPRAKDDRRRKSRPKVSAFSD PTPPADQQPGHQKNLHAHNHPKKRNRHRCSSPPPPHEFRAYQLYTLYRGKDGKVMQAPINGCRCEPLINK LENQLEATVEEIKAELGSVQDKMNTKLGQMENKTQHQMRVLDKLMVERLSAERTECLNRLQQHSDTEKHE GEKRQISLVDELKTWCMLKIQNLEQKLSGDSRACRAKSTPSTCESSTGVDQLVVTAGPAAASDSSPPVVR PKEKALNSTATQRLQQELSSSDCTGSRLRNVKVQTALLPMNEAARSDQQAGPCVNRGTQTKKSGKSGPTR HRAQQPAASSTCGQPPPATGSEQTGPHIRDTSQALELTQYFFEAVSTQMEKWYERKIEEARSQANQKAQQ DKATLKEHIKSLEEELAKLRTRVQKEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242809
ORF Size	2181 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242809.2
RefSeq Size	5355 bp
RefSeq ORF	2184 bp
Locus ID	22881
UniProt ID	Q9Y2G4
MW	80.4 kDa
Gene Summary	Recruits CKI-epsilon to the beta-catenin degradation complex that consists of AXN1 or AXN2 and GSK3-beta and allows efficient phosphorylation of beta-catenin, thereby inhibiting beta-catenin/Tcf signals.[UniProtKB/Swiss-Prot Function]

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