NBPF3 (NM_001256416) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AE2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234587 representing NM_001256416 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACTGACTCCCACTGTCCAGGGCTTCCAGTGGACTCTCCGAGGCCCTGATGTAGAAACTTCCCCAT TCGGTGCACCAAGAGCAGCCTCACATGGTGTGGGCCGACATCAAGAGCTGCGAGATCCAACAGTCCCTGG CCCCACCTCTTCTGCCACAAACGTCAGCATGGTGGTATCTGCCGGCCCTTGGTCCGGTGAGAAGGCAGAG ATGAACATTCTAGAAATCAACAAGAAATCGCGCCCCCAGCTGGCAGAGAACAAACAGCAGTTCAGAAACC TCAAACAGAAATGTCTTGTAACTCAAGTGGCCTACTTCCTGGCCAACCGGCAAAATAATTACGACTATGA AGACTGCAAAGACCTCATAAAATCTATGCTGAGGGATGAGCGGCTGCTCACAGAAGAGAAGCTTGCAGAG GAGCTCGGGCAAGCTGAGGAGCTCAGGCAATATAAAGTCCTGGTTCACTCTCAGGAACGAGAGCTGACCC AGTTAAGGGAGAAGTTACAGGAAGGGAGAGATGCCTCCCGCTCATTGAATCAGCATCTCCAGGCCCTCCT CACTCCGGATGAGCCGGACAACTCCCAGGGACGGGACCTCCGAGAACAGCTGGCTGAGGGATGTAGGCTG GCACAGCACCTCGTCCAAAAGCTCAGCCCAGAAAATGATGACGATGAGGATGAAGATGTTAAAGTTGAGG AGGCTGAGAAAGTACAGGAATTATATGCCCCCAGGGAGGTGCAGAAGGCTGAAGAAAAGGAAGTCCCTGA GGACTCACTGGAGGAGTGTGCCATCACTTGTTCAAATAGCCACCACCCTTGTGAGTCCAACCAGCCTTAC GGGAACACCAGAATCACATTTGAGGAAGACCAAGTCGACTCAACTCTCATTGACTCATCCTCTCATGATG AATGGTTGGATGCTGTATGCATTATCCCAGAAAATGAAAGTGATCATGAGCAAGAGGAAGAAAAAGGGCC AGTGTCTCCCAGATCATCTGGAAGGTTTTGTTGTCTAATCTCTGTTGGTTATATCTTCTGTCATCCCTGT CCTGCCTGGCTCATCAGGAATCTGCAGGAGTCTGAAGAGGAGGAAGCCCCCCAGGAGTCCTGGGATGAAG GACATTGGTGTGATCAAGTGAAAAAGGAGGACCAAGAGGCCACAAGTCCCAGGCTCAGCAGGGAGCTGCT GGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATTTTATTCAACTCCTTTTGAGTACCTG GAACTGCCTGACTTATGCCAGCCCTACAGAAGTGACTTTTACTCATTGCAGGAACAACACCTTGGCTTGG CTCTTGACTTGGACAGAATGAAAAAGGACCAAGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCT CAGCAGAGAGCTGCCGGAGGTAGTAGAGCCTGAGGACTTGCAGGACTCACTGGATAGATGGTATTCGACT CCTTTCAGTTATCCAGAACTGCCTGATTCATGCCAGCCCTACGGAAGTTGCTTTTACTCATTGGAGGAAG AACACGTTGGCTTTTCTCTTGACGTGGATGAAATTGAAAAGTACCAAGAAGGGGAAGAAGATCAAAAGCC ACCATGCCCCAGGCTCAACGAGGTGCTGATGGAAGCAGAAGAGCCTGAAGTCTTGCAGGACTCACTGGAT AGATGTTATTCGACTACTTCAACTTACTTTCAACTACATGCCTCATTCCAGCAGTATAGAAGTGCCTTTT ACTCATTTGAGGAACAGGACGTCAGCTTGGCCCTTGACGTGGACAATAGGTTTTTTACTTTGACAGTGAT AAGGCACCACCTGGCCTTCCAGATGGGAGTCATATTCCCACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234587 representing NM_001256416 Red=Cloning site Green=Tags(s) MPLTPTVQGFQWTLRGPDVETSPFGAPRAASHGVGRHQELRDPTVPGPTSSATNVSMVVSAGPWSGEKAE MNILEINKKSRPQLAENKQQFRNLKQKCLVTQVAYFLANRQNNYDYEDCKDLIKSMLRDERLLTEEKLAE ELGQAEELRQYKVLVHSQERELTQLREKLQEGRDASRSLNQHLQALLTPDEPDNSQGRDLREQLAEGCRL AQHLVQKLSPENDDDEDEDVKVEEAEKVQELYAPREVQKAEEKEVPEDSLEECAITCSNSHHPCESNQPY GNTRITFEEDQVDSTLIDSSSHDEWLDAVCIIPENESDHEQEEEKGPVSPRSSGRFCCLISVGYIFCHPC PAWLIRNLQESEEEEAPQESWDEGHWCDQVKKEDQEATSPRLSRELLDEKEPEVLQDSLDRFYSTPFEYL ELPDLCQPYRSDFYSLQEQHLGLALDLDRMKKDQEEEEDQGPPCPRLSRELPEVVEPEDLQDSLDRWYST PFSYPELPDSCQPYGSCFYSLEEEHVGFSLDVDEIEKYQEGEEDQKPPCPRLNEVLMEAEEPEVLQDSLD RCYSTTSTYFQLHASFQQYRSAFYSFEEQDVSLALDVDNRFFTLTVIRHHLAFQMGVIFPH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256416
ORF Size	1863 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256416.4
RefSeq Size	3795 bp
RefSeq ORF	1866 bp
Locus ID	84224
UniProt ID	Q9H094
MW	72.1 kDa
Gene Summary	This gene is a member of the neuroblastoma breakpoint family (NBPF) which consists of dozens of recently duplicated genes primarily located in segmental duplications on human chromosome 1. This gene family has experienced its greatest expansion within the human lineage and has expanded, to a lesser extent, among primates in general. Members of this gene family are characterized by tandemly repeated copies of DUF1220 protein domains. DUF1220 copy number variations in human chromosomal region 1q21.1, where most DUF1220 domains are located, have been implicated in a number of developmental and neurogenetic diseases such as microcephaly, macrocephaly, autism, schizophrenia, cognitive disability, congenital heart disease, neuroblastoma, and congenital kidney and urinary tract anomalies. Altered expression of some gene family members is associated with several types of cancer. This gene family contains numerous pseudogenes. [provided by RefSeq, Feb 2013]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.