USP10 (NM_001272075) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	UBPO
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233142 representing NM_001272075 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTGGTTGCCCTCTCCTGGAATAGGGCAGTATATTTTTGGAGATTTTAGCCCTGATGAATTCAATC AATTCTTTGTGACTCCTCGATCTTCAGTTGAGCTTCCTCCATACAGTGGAACAGTTCTGTGTGGCACACA GGCTGTGGATAAACTACCTGATGGACAAGAATATCAGAGAATTGAGTTTGGTGTCGATGAAGTCATTGAA CCCAGTGACACTTTGCCGAGAACCCCCAGCTACAGTATTTCAAGCACACTGAACCCTCAGGCCCCTGAAT TTATTCTCGGTTGTACAGCTTCCAAAATAACCCCTGATGGTATCACTAAAGAAGCAAGCTATGGCTCCAT CGACTGCCAGTACCCAGGCTCTGCCCTCGCTTTGGATGGAAGTTCTAATGTGGAGGCGGAAGTTTTGGAA AATGATGGTGTCTCAGGTGGTCTTGGACAAAGGGAGCGTAAAAAGAAGAAAAAGCGGCCACCTGGATATT ACAGCTATTTGAAAGATGGTGGCGATGATAGTATCTCCACAGAAGCCCTGGTCAATGGCCATGCCAATTC AGCAGTCCCGAACAGTGTCAGTGCAGAGGATGCAGAATTTATGGGTGACATGCCCCCGTCAGTTACGCCC AGGACTTGTAACAGCCCCCAGAACTCCACAGACTCTGTCAGTGACATTGTGCCTGACAGTCCTTTCCCCG GAGCACTCGGCAGTGACACCAGGACTGCAGGGCAGCCAGAGGGGGGCCCCGGGGCTGATTTTGGTCAGTC CTGCTTCCCTGCAGAGGCTGGCAGAGACACCCTGTCAAGGACAGCTGGGGCTCAGCCCTGCGTTGGTACC GATACTACTGAAAACCTTGGAGTTGCTAATGGACAAATACTTGAATCCTCGGGTGAGGGCACAGCTACCA ACGGGGTGGAGTTGCACACCACGGAAAGCATAGACTTGGACCCAACCAAACCCGAGAGTGCATCACCTCC TGCTGACGGCACGGGCTCTGCATCAGGCACCCTTCCTGTCAGCCAGCCCAAGTCCTGGGCCAGCCTCTTT CATGATTCTAAGCCCTCTTCCTCCTCGCCGGTGGCCTATGTGGAAACTAAGTATTCCCCTCCCGCCATAT CTCCCCTGGTTTCTGAAAAGCAGGTTGAAGTCAAAGAAGGGCTTGTTCCGGTTTCAGAGGATCCTGTAGC CATAAAGATTGCAGAGTTGCTGGAGAATGTAACCCTAATCCATAAACCAGTGTCGTTGCAACCCCGTGGG CTGATCAATAAAGGGAACTGGTGCTACATTAATGCTACACTGCAGGCATTGGTTGCTTGCCCGCCGATGT ACCACCTGATGAAGTTCATTCCTCTGTATTCCAAAGTGCAAAGGCCTTGTACGTCAACACCCATGATAGA CAGCTTTGTTCGGCTAATGAATGAGTTCACTAATATGCCAGTACCTCCAAAACCCCGACAAGCTCTTGGA GATAAAATCGTGAGGGATATTCGCCCTGGAGCTGCCTTTGAGCCCACATATATTTACAGACTCCTGACAG TTAACAAGTCAAGCCTGTCTGAAAAGGGTCGACAAGAAGATGCTGAGGAATACTTAGGCTTCATTCTAAA TGGACTTCATGAGGAAATGTTGAACCTAAAGAAGCTTCTCTCACCAAGTAATGAAAAACTTACGATTTCC AACGGCCCCAAAAACCACTCGGTCAATGAAGAAGAGCAGGAAGAACAAGGTGAAGGAAGCGAGGATGAAT GGGAACAAGTGGGCCCCCGGAACAAGACTTCCGTCACCCGCCAGGCGGATTTTGTTCAGACTCCAATCAC CGGCATTTTTGGTGGACACATCAGGTCTGTGGTTTACCAGCAGAGTTCAAAAGAATCTGCCACTTTGCAG CCATTTTTCACGTTGCAGTTGGATATCCAGTCAGACAAGATACGCACAGTCCAGGATGCACTGGAGAGCT TGGTGGCAAGAGAATCTGTCCAAGGTTATACCACAAAAACCAAACAAGAGGTTGAGATAAGTCGAAGAGT GACTCTGGAAAAACTCCCTCCTGTCCTCGTGCTGCACCTGAAACGATTCGTTTATGAGAAGACTGGTGGG TGCCAGAAGCTTATCAAAAATATTGAATATCCTGTGGACTTGGAAATTAGTAAAGAACTGCTTTCTCCAG GGGTTAAAAATAAGAATTTTAAATGCCACCGAACCTATCGGCTCTTTGCAGTGGTCTACCATCACGGCAA CAGTGCGACGGGCGGCCATTACACTACAGACGTCTTCCAGATCGGTCTGAATGGCTGGCTGCGCATCGAT GACCAGACAGTCAAGGTGATCAACCAGTACCAGGTGGTGAAACCAACTGCTGAACGCACAGCCTACCTCC TGTATTACCGCCGAGTGGACCTGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233142 representing NM_001272075 Red=Cloning site Green=Tags(s) MPWLPSPGIGQYIFGDFSPDEFNQFFVTPRSSVELPPYSGTVLCGTQAVDKLPDGQEYQRIEFGVDEVIE PSDTLPRTPSYSISSTLNPQAPEFILGCTASKITPDGITKEASYGSIDCQYPGSALALDGSSNVEAEVLE NDGVSGGLGQRERKKKKKRPPGYYSYLKDGGDDSISTEALVNGHANSAVPNSVSAEDAEFMGDMPPSVTP RTCNSPQNSTDSVSDIVPDSPFPGALGSDTRTAGQPEGGPGADFGQSCFPAEAGRDTLSRTAGAQPCVGT DTTENLGVANGQILESSGEGTATNGVELHTTESIDLDPTKPESASPPADGTGSASGTLPVSQPKSWASLF HDSKPSSSSPVAYVETKYSPPAISPLVSEKQVEVKEGLVPVSEDPVAIKIAELLENVTLIHKPVSLQPRG LINKGNWCYINATLQALVACPPMYHLMKFIPLYSKVQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALG DKIVRDIRPGAAFEPTYIYRLLTVNKSSLSEKGRQEDAEEYLGFILNGLHEEMLNLKKLLSPSNEKLTIS NGPKNHSVNEEEQEEQGEGSEDEWEQVGPRNKTSVTRQADFVQTPITGIFGGHIRSVVYQQSSKESATLQ PFFTLQLDIQSDKIRTVQDALESLVARESVQGYTTKTKQEVEISRRVTLEKLPPVLVLHLKRFVYEKTGG CQKLIKNIEYPVDLEISKELLSPGVKNKNFKCHRTYRLFAVVYHHGNSATGGHYTTDVFQIGLNGWLRID DQTVKVINQYQVVKPTAERTAYLLYYRRVDLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001272075
ORF Size	2406 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001272075.2
RefSeq Size	3520 bp
RefSeq ORF	2409 bp
Locus ID	9100
UniProt ID	Q14694
Protein Families	Druggable Genome, Protease
MW	88 kDa
Gene Summary	Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. Several transcript variants, some protein-coding and others not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2013]

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