CLPB (NM_001258394) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ANKCLB; HSP78; MEGCANN; MGCA7; SKD3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233061 representing NM_001258394 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCGCGGCTGCCACTTGGGGACGCCTTCCTGGTCCCGAAGAAACACTCCCAGGACAGGACAGCTGG AACGGGGTCCCCAGCAGGGCCGGACTGGGCATGTGCGCCCTGGCCGCAGCGCTGGTGGTTCATTGCTACA GCAAGAGTCCGTCCAACAAGGATGCAGCCCTGTTGGAAGCTGCCCGTGCCAACAATATGCAAGAAGTCAG CAGCCCCAAGAGACTGCAAAGAATGATGCTCAGTCAAGAAGCTGGGCAGGGCTGAATGCTGGAGTCTCAC TGAAGAACACCAAGATTTCCTCCTCTGAATGGCCCCTCAGGCTGTTGTCAGAAGGTGCAGATGTCAATGC AAAGCACAGACTTGGCTGGACAGCACTCATGGTGGCAGCCATCAACCGAAACAACAGTGTGGTACAGGTC CTGCTTGCTGCTGGGGCTGATCCAAACCTTGGAGATGATTTCAGCAGTGTTTACAAGACTGCCAAGGAAC AGGGAATCCATTCTTTGGAAGATGGGGGACAGGACGGTGCAAGCCGGCACATCACAAACCAGTGGACAAG TGCCCTGGAGTTCAGGAGATGGCTAGGACTCCCCGCTGGCGTCCTGATCACCCGAGAGGATGACTTCAAC AACAGGCTGAACAACCGCGCCAGTTTCAAGGGCTGCACGGCCTTGCACTATGCTGTTCTTGCTGATGACT ACCGCACTGTCAAGGAGCTGCTTGATGGAGGAGCCAACCCCCTGCAGAGGAATGAAATGGGACACACACC CTTGGATTATGCCCGAGAAGGGGAAGTGATGAAGCTTCTGAGGACTTCTGAAGCCAAGTACCAAGAGAAG CAGCGGAAGCGTGAGGCTGAGGAGCGGCGCCGCTTCCCCCTGGAGCAGCGACTAAAGGAGCACATCATTG GCCAGGAGAGCGCCATCGCCACAGTGGGTGCTGCGATCCGGAGGAAGGAGAATGGCTGGTACGATGAAGA ACACCCTCTGGTCTTCCTCTTCTTGGGATCATCTGGAATAGGAAAAACAGAGCTGGCCAAGCAGACAGCC AAATATATGCACAAAGATGCTAAAAAGGGCTTCATCAGGCTGGACATGTCCGAGTTCCAGGAGCGACACG AGGTGGCCAAGTTTATTGGGTCTCCACCAGGCTACGTTGGCCATGAGGAGGGTGGCCAGCTGACCAAGAA GTTGAAGCAGTGCCCCAATGCTGTGGTGCTCTTTGATGAAGTAGACAAGGCCCATCCAGATGTGCTCACC ATCATGCTGCAGCTGTTTGATGAGGGCCGGCTGACAGATGGAAAAGGGAAGACCATTGATTGCAAGGACG CCATCTTCATCATGACCTCCAATGTGGCCAGCGACGAGATCGCACAGCACGCGCTGCAGCTGAGGCAGGA AGCTTTGGAGATGAGCCGTAACCGTATTGCCGAAAACCTGGGGGATGTCCAGATAAGTGACAAGATCACC ATCTCAAAGAACTTCAAGGAGAATGTGATTCGCCCTATCCTGAAAGCTCACTTCCGGAGGGATGAGTTTC TGGGACGGATCAATGAGATCGTCTACTTCCTCCCCTTCTGCCACTCGGAGCTCATCCAACTCGTCAACAA GGAACTAAACTTCTGGGCCAAGAGAGCCAAGCAAAGGCACAACATCACGCTGCTCTGGGACCGCGAGGTG GCAGATGTGCTGGTCGACGGCTACAATGTGCACTATGGCGCCCGCTCCATCAAACATGAGGTAGAACGCC GTGTGGTGAACCAGCTGGCAGCAGCCTATGAGCAGGACCTGCTGCCAGGGGGCTGTACTTTGCGCATCAC GGTGGAGGACTCAGACAAGCAGCTACTCAAAAGCCCAGAACTGCCCTCACCCCAGGCTGAGAAGCGCCTC CCCAAGCTGCGTCTGGAGATCATCGACAAGGACAGCAAGACTCGCAGACTGGACATCCGGGCACCACTGC ACCCTGAGAAGGTGTGCAACACCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233061 representing NM_001258394 Red=Cloning site Green=Tags(s) MPRGCHLGTPSWSRRNTPRTGQLERGPQQGRTGHVRPGRSAGGSLLQQESVQQGCSPVGSCPCQQYARSQ QPQETAKNDAQSRSWAGLNAGVSLKNTKISSSEWPLRLLSEGADVNAKHRLGWTALMVAAINRNNSVVQV LLAAGADPNLGDDFSSVYKTAKEQGIHSLEDGGQDGASRHITNQWTSALEFRRWLGLPAGVLITREDDFN NRLNNRASFKGCTALHYAVLADDYRTVKELLDGGANPLQRNEMGHTPLDYAREGEVMKLLRTSEAKYQEK QRKREAEERRRFPLEQRLKEHIIGQESAIATVGAAIRRKENGWYDEEHPLVFLFLGSSGIGKTELAKQTA KYMHKDAKKGFIRLDMSEFQERHEVAKFIGSPPGYVGHEEGGQLTKKLKQCPNAVVLFDEVDKAHPDVLT IMLQLFDEGRLTDGKGKTIDCKDAIFIMTSNVASDEIAQHALQLRQEALEMSRNRIAENLGDVQISDKIT ISKNFKENVIRPILKAHFRRDEFLGRINEIVYFLPFCHSELIQLVNKELNFWAKRAKQRHNITLLWDREV ADVLVDGYNVHYGARSIKHEVERRVVNQLAAAYEQDLLPGGCTLRITVEDSDKQLLKSPELPSPQAEKRL PKLRLEIIDKDSKTRRLDIRAPLHPEKVCNTI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001258394
ORF Size	1986 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258394.2
RefSeq Size	3379 bp
RefSeq ORF	1989 bp
Locus ID	81570
UniProt ID	Q9H078
MW	75 kDa
Gene Summary	This gene belongs to the ATP-ases associated with diverse cellular activities (AAA+) superfamily. Members of this superfamily form ring-shaped homo-hexamers and have highly conserved ATPase domains that are involved in various processes including DNA replication, protein degradation and reactivation of misfolded proteins. All members of this family hydrolyze ATP through their AAA+ domains and use the energy generated through ATP hydrolysis to exert mechanical force on their substrates. In addition to an AAA+ domain, the protein encoded by this gene contains a C-terminal D2 domain, which is characteristic of the AAA+ subfamily of Caseinolytic peptidases to which this protein belongs. It cooperates with Hsp70 in the disaggregation of protein aggregates. Allelic variants of this gene are associated with 3-methylglutaconic aciduria, which causes cataracts and neutropenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.