INT11 (CPSF3L) (NM_001256462) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CPSF3L; CPSF73L; INT11; RC-68; RC68
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232873 representing NM_001256462 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGAGATCAGAGTCACGCCCTTGGGTGCCTGCCCTGTGCTGTCCTGGAACCTGGTCCTTCCACCGG GCAGGGGGTCGAGGGCACCTTCCAGCACAGACACCAGGACGGGATTCCATGTCCTCCCCCATGGCGTGAA TGATGAGCTGGAGATCAAGGCCTACTATGCAGGCCACGTGCTGGGGGCAGCCATGTTCCAGATTAAAGTG GGCTCAGAGTCTGTGGTCTACACGGGTGATTATAACATGACCCCAGACCGACACTTAGGAGCTGCCTGGA TTGACAAGTGCCGCCCCAACCTGCTCATCACAGAGTCCACGTACGCCACGACCATCCGTGACTCCAAGCG CTGCCGGGAGCGAGACTTCCTGAAGAAAGTCCACGAGACCGTGGAGCGTGGTGGGAAGGTGCTGATACCT GTGTTCGCGCTGGGCCGCGCCCAGGAGCTCTGCATCCTCCTGGAGACCTTCTGGGAGCGCATGAACCTGA AGGTGCCCATCTACTTCTCCACGGGGCTGACCGAGAAGGCCAACCACTACTACAAGCTGTTCATCCCCTG GACCAACCAGAAGATCCGCAAGACTTTCGTGCAGAGGAACATGTTTGAGTTCAAGCACATCAAGGCCTTC GACCGGGCTTTTGCTGACAACCCAGGACCGATGGTTGTGTTTGCCACGCCAGGAATGCTGCACGCTGGGC AGTCCCTGCAGATCTTCCGGAAATGGGCCGGAAACGAAAAGAACATGGTCATCATGCCCGGCTACTGCGT GCAGGGCACCGTCGGCCACAAGATCCTCAGCGGGCAGCGGAAGCTCGAGATGGAGGGGCGGCAGGTGCTG GAGGTCAAGATGCAGGTGGAGTACATGTCATTCAGCGCACACGCGGACGCCAAGGGCATCATGCAGCTGG TGGGCCAGGCAGAGCCGGAGAGCGTGCTGCTGGTGCATGGCGAGGCCAAGAAGATGGAGTTCCTGAAGCA GAAGATCGAGCAGGAGCTCCGGGTCAACTGCTACATGCCGGCCAATGGCGAGACGGTGACGCTGCCCACA AGCCCCAGCATCCCCGTAGGCATCTCGCTGGGGCTGCTGAAGCGGGAGATGGCGCAGGGGCTGCTCCCTG AGGCCAAGAAGCCTCGGCTCCTGCACGGCACCCTGATCATGAAGGACAGCAACTTCCGGCTGGTGTCCTC AGAGCAAGCCCTCAAAGAGCTGGGTCTGGCTGAGCACCAGCTGCGCTTCACCTGCCGCGTGCACCTGCAT GACACACGCAAGGAGCAGGAGACGGCATTGCGCGTCTACAGCCACCTCAAGAGCGTCCTGAAGGACCACT GTGTGCAGCACCTCCCAGACGGCTCTGTGACTGTGGAGTCCGTCCTCCTCCAGGCCGCCGCCCCTTCTGA GGACCCAGGCACCAAGGTGCTGCTGGTCTCCTGGACCTACCAGGACGAGGAGCTGGGGAGCTTCCTCACA TCTCTGCTGAAGAAGGGCCTCCCCCAGGCCCCCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232873 representing NM_001256462 Red=Cloning site Green=Tags(s) MPEIRVTPLGACPVLSWNLVLPPGRGSRAPSSTDTRTGFHVLPHGVNDELEIKAYYAGHVLGAAMFQIKV GSESVVYTGDYNMTPDRHLGAAWIDKCRPNLLITESTYATTIRDSKRCRERDFLKKVHETVERGGKVLIP VFALGRAQELCILLETFWERMNLKVPIYFSTGLTEKANHYYKLFIPWTNQKIRKTFVQRNMFEFKHIKAF DRAFADNPGPMVVFATPGMLHAGQSLQIFRKWAGNEKNMVIMPGYCVQGTVGHKILSGQRKLEMEGRQVL EVKMQVEYMSFSAHADAKGIMQLVGQAEPESVLLVHGEAKKMEFLKQKIEQELRVNCYMPANGETVTLPT SPSIPVGISLGLLKREMAQGLLPEAKKPRLLHGTLIMKDSNFRLVSSEQALKELGLAEHQLRFTCRVHLH DTRKEQETALRVYSHLKSVLKDHCVQHLPDGSVTVESVLLQAAAPSEDPGTKVLLVSWTYQDEELGSFLT SLLKKGLPQAPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256462
ORF Size	1506 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256462.1, NP_001243391.1
RefSeq Size	1874 bp
RefSeq ORF	1509 bp
Locus ID	54973
UniProt ID	Q5TA45
MW	56.8 kDa
Gene Summary	The Integrator complex contains at least 12 subunits and associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates the 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690). INTS11, or CPSF3L, is the catalytic subunit of the Integrator complex (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM, Mar 2008]

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