FRMD3 (NM_001244961) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4.1O; EPB41L4O; EPB41LO; P410
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232581 representing NM_001244961 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTTCAGAAGGGGGCAGAGCCCACCAGTTGCTGAATTTAACTTGCTCCTGAAAGCTCACACTTTGG AAACCTACGGGGTGGATCCTCACCCATGCAAGGATTCAACAGGCACAACAACATTTTTAGGATTCACAGC TGCAGGCTTTGTGGTCTTTCAGGGAAATAAGAGAATCCATTTGATAAAATGGCCAGATGTCTGCAAATTG AAGTTTGAAGGGAAGACATTTTATGTGATTGGCACCCAGAAGGAGAAAAAAGCCATGTTGGCATTCCATA CTTCAACACCAGCTGCCTGCAAACATCTTTGGAAGTGTGGAGTGGAAAACCAGGCCTTTTATAAGTATGC AAAATCCAGTCAGATCAAGACTGTATCAAGCAGCAAGATATTTTTTAAAGGAAGTAGATTTCGATATAGT GGGAAAGTTGCCAAAGAGGTGGTGGAGGCCAGTTCCAAGATCCAGAGGGAGCCTCCTGAGGTGCACAGAG CCAACATTACTCAGAGCCGCAGTTCCCACTCCTTGAACAAACAGCTCATCATTAACATGGAACCCCTGCA GCCCCTGCTTCCTTCCCCCAGCGAGCAAGAAGAAGAACTTCCTCTGGGTGAGGGTGTTCCATTGCCTAAA GAGGAGAACATTTCTGCTCCCTTGATCTCCAGCTCCCCAGTGAAGGCAGCCCGGGAGTATGAAGATCCCC CTAGTGAAGAGGAAGATAAAATAAAAGAAGAACCTTTAACCATCTCTGAACTAGTGTACAACCCAAGTGC CAGCCTGCTCCCCACCCCTGTGGATGACGATGAGATTGACATGCTCTTTGACTGTCCTTCTAGGCTTGAG TTGGAAAGAGAAGACACAGATTCATTTGAGGATCTGGAAGCAGATGAAAACGCCTTTTTGATTGCTGAAG AAGAGGAGCTGAAGGAGGCTCGCCGTGCTTTGTCGTGGAGCTATGACATTCTGACTGGCCATATTCGGGT GAACCCACTGGTCAAGAGTTTTTCCAGGCTCCTTGTGGTGGGCCTGGGACTGCTGCTCTTTGTATTTCCC CTGCTCCTCCTCCTTTTGGAGTCAGTCTCCATGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232581 representing NM_001244961 Red=Cloning site Green=Tags(s) MVFRRGQSPPVAEFNLLLKAHTLETYGVDPHPCKDSTGTTTFLGFTAAGFVVFQGNKRIHLIKWPDVCKL KFEGKTFYVIGTQKEKKAMLAFHTSTPAACKHLWKCGVENQAFYKYAKSSQIKTVSSSKIFFKGSRFRYS GKVAKEVVEASSKIQREPPEVHRANITQSRSSHSLNKQLIINMEPLQPLLPSPSEQEEELPLGEGVPLPK EENISAPLISSSPVKAAREYEDPPSEEEDKIKEEPLTISELVYNPSASLLPTPVDDDEIDMLFDCPSRLE LEREDTDSFEDLEADENAFLIAEEEELKEARRALSWSYDILTGHIRVNPLVKSFSRLLVVGLGLLLFVFP LLLLLLESVSMQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001244961
ORF Size	1086 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001244961.1, NP_001231890.1
RefSeq Size	1415 bp
RefSeq ORF	1089 bp
Locus ID	257019
UniProt ID	A2A2Y4
Protein Families	Transmembrane
MW	41.1 kDa
Gene Summary	The protein encoded by this gene is a single pass membrane protein primarily found in ovaries. A similar protein in erythrocytes helps determine the shape of red blood cells, but the function of the encoded protein has not been determined. There is some evidence that this is a tumor suppressor gene, and there is also evidence linking defects in this gene to susceptibility to diabetic nephropathy in type 1 diabetes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

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