C16orf57 (USB1) (NM_001204911) Human Tagged ORF Clone
CAT#: RC232018
- TrueORF®
USB1 (Myc-DDK tagged) - Homo sapiens U6 snRNA biogenesis 1 (USB1), transcript variant 3
ORF Plasmid: tGFP
"NM_001204911" in other vectors (2)
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CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C16orf57; hUsb1; HVSL1; Mpn1; PN |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC232018 representing NM_001204911
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGCGGCGCCCCTGGTGGGCTACAGCAGCAGCGGCTCCGAGGATGAGTCCGAGGACGGGATGCGGA CCAGGCCGGGGGATGGGAGCCACCGTCGTGGCCAGAGCCCCCTTCCCAGGCAGAGATTTCCAGTACCTGA CAGTGTGCTGAACATGTTCCCGGGCACCGAGGAGGGGCCTGAAGATGACAGCACAAAACACGGGGGACGG GTGCGCACCTTCCCCCACGAGCGAGGCAACTGGGCCACCCACGTCTATGTACCATATGAAGCCAAGGAGG AGTTCCTGGATCTGCTTGATGTGTTGCTGCCCCATGCCCAGACATATGTCCCCCGGCTGGTAAGGATGAA GGTGTTCCACCTCAGCCTGTCCCAGAGTGTGGTTCTGCGCCACCACTGGATCCTCCCCTTCGTGCAGGCT CTGAAAGCCCGTATGACCTCCTTCCACAGCCCCCATCCAGGACCTCATTGCCTCATTGGAACCAAAGATG CCCCTGTCACCCAGGAAATTCCAAAGGATTTAGGAGCTCTGCGTCAGGAGCCAGGATCACAGACCAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC232018 representing NM_001204911
Red=Cloning site Green=Tags(s) MSAAPLVGYSSSGSEDESEDGMRTRPGDGSHRRGQSPLPRQRFPVPDSVLNMFPGTEEGPEDDSTKHGGR VRTFPHERGNWATHVYVPYEAKEEFLDLLDVLLPHAQTYVPRLVRMKVFHLSLSQSVVLRHHWILPFVQA LKARMTSFHSPHPGPHCLIGTKDAPVTQEIPKDLGALRQEPGSQTR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001204911 |
ORF Size | 558 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001204911.2 |
RefSeq Size | 3113 bp |
RefSeq ORF | 561 bp |
Locus ID | 79650 |
UniProt ID | Q9BQ65 |
MW | 21.3 kDa |
Gene Summary | This gene encodes a protein with several conserved domains, however, its exact function is not known. Mutations in this gene are associated with poikiloderma with neutropenia (PN), which shows phenotypic overlap with Rothmund-Thomson syndrome (RTS) caused by mutations in the RECQL4 gene. It is believed that this gene product interacts with RECQL4 protein via SMAD4 proteins, explaining the partial clinical overlap between PN and RTS. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011] |
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