NEK4 (NM_001193533) Human Tagged ORF Clone

CAT＃: RC231401

TrueORF^®

NEK4 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)-related kinase 4 (NEK4), transcript variant 2

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_001193533" in other vectors (3)

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CNY 7,510.00

货期*

4周

规格

Product images

经常一起买 (5)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

TA350920

Rabbit Polyclonal Anti-NEK4 Antibody

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NRK2; pp12301; STK2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC231401 representing NM_001193533 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCTGGCCGCCTACTGCTACCTGCGGGTCGTGGGCAAGGGGAGCTATGGAGAGGTGACGCTTGTGA AGCACCGGCGGGACGGCAAGCAGTATTTACATGAAAAACACATCCTTCATCGAGATCTGAAAACTCAAAA TGTCTTCCTAACAAGAACAAACATCATCAAAGTAGGGGACCTAGGAATTGCCCGAGTGTTAGAGAACCAC TGTGACATGGCTAGCACCCTCATTGGCACACCCTACTACATGAGCCCTGAATTGTTCTCAAACAAACCCT ACAACTATAAGTCTGATGTTTGGGCTCTAGGATGCTGTGTCTATGAAATGGCCACCTTGAAGCATGCTTT CAATGCAAAAGATATGAATTCTTTAGTTTATCGGATTATTGAAGGAAAGCTGCCACCAATGCCAAGAGAT TACAGCCCAGAGCTGGCAGAACTGATAAGAACAATGCTGAGCAAAAGGCCTGAAGAAAGGCCGTCTGTGA GGAGCATCCTGAGGCAGCCTTATATAAAGCGGCAAATCTCCTTCTTTTTGGAGGCCACAAAGATAAAAAC CTCCAAAAATAACATTAAAAATGGTGACTCTCAATCCAAGCCTTTTGCTACAGTGGTTTCTGGAGAGGCA GAATCAAATCATGAAGTAATCCACCCCCAACCACTCTCTTCTGAGGGCTCCCAGACATATATAATGGGTG AAGGCAAATGTTTGTCCCAGGAGAAACCCAGGGCCTCTGGTCTCTTGAAGTCACCTGCCAGTCTGAAAGC CCATACCTGCAAACAGGACTTGAGCAATACCACAGAACTAGCCACAATCAGTAGCGTAAATATTGACATC TTACCTGCAAAAGGGAGGGATTCAGTGAGTGATGGCTTTGTTCAGGAGAATCAGCCAAGATATTTGGATG CCTCTAATGAGTTAGGAGGTATATGCAGTATTTCTCAAGTGGAAGAGGAGATGCTGCAGGACAACACTAA ATCCAGTGCCCAGCCTGAAAACCTGATTCCCATGTGGTCCTCTGACATTGTCACTGGGGAAAAGAATGAA CCAGTGAAGCCTCTGCAGCCCCTAATCAAAGAACAAAAGCCAAAGGACCAGAGTCTTGCCCTGTCGCCCA AGCTGGAGTGCAGTGGCACAATCTTGGCTCACAGCAACCTCCGCCTCCTGGGTTCAAGTGATTCTCCAGC CTCAGCCTCCCGAGTAGCTGGGATTACAGGCGTGTGCCACCACGCCCAGGATCAAGTTGCTGGTGAATGT ATTATAGAAAAACAGGGCAGAATCCACCCAGATTTACAGCCACACAACTCTGGGTCTGAACCTTCCCTGT CTCGACAGCGACGGCAAAAGAGGAGAGAACAGACTGAGCACAGAGGGGAAAAGAGACAGGTCCGCAGAGA TCTCTTTGCTTTCCAAGAGTCGCCTCCTCGATTTTTGCCTTCTCATCCCATTGTTGGGAAAGTGGATGTC ACATCAACACAAAAAGAGGCTGAAAACCAACGTAGAGTGGTCACTGGGTCTGTGAGCAGTTCAAGGAGCA GTGAGATGTCATCATCAAAGGATCGACCATTATCAGCCAGAGAGAGGAGGCGACTAAAGCAGTCACAGGA AGAAATGTCCTCTTCAGGCCCTTCAGTGAGGAAAGCGTCTCTGAGTGTAGCAGGGCCAGGAAAACCCCAG GAAGAAGACCAGCCCTTGCCTGCCCGACGGCTCTCCTCTGACTGCAGCGTCACTCAGGAAAGGAAACAGA TTCATTGTCTGTCTGAGGATGAGTTAAGTTCTTCTACAAGTTCAACTGATAAGTCAGATGGGGATTACGG GGAAGGGAAAGGTCAGACAAATGAAATTAATGCCTTGGTACAATTGATGACTCAGACCCTGAAACTGGAT TCTAAAGAGAGCTGTGAAGATGTCCCGGTAGCAAACCCAGTGTCAGAATTCAAACTTCATCGGAAATATC GGGACACACTGATACTTCATGGGAAGGTTGCAGAAGAGGCAGAGGAAATCCATTTTAAAGAGCTACCTTC AGCTATTATGCCAGGTTCTGAAAAGATCAGGAGACTAGTTGAAGTCTTGAGAACTGATGTAATTCGTGGC CTGGGAGTTCAGCTTTTAGAGCAGGTGTATGATCTTTTGGAGGAGGAGGATGAATTTGATAGAGAGGTAC GTTTGCGGGAGCACATGGGTGAAAAGTATACAACTTACAGTGTGAAAGCTCGCCAGTTGAAATTTTTTGA AGAAAACATGAATTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC231401 representing NM_001193533 Red=Cloning site Green=Tags(s) MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENH CDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRD YSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEA ESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDI LPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNE PVKPLQPLIKEQKPKDQSLALSPKLECSGTILAHSNLRLLGSSDSPASASRVAGITGVCHHAQDQVAGEC IIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDV TSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQ EEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLD SKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRG LGVQLLEQVYDLLEEEDEFDREVRLREHMGEKYTTYSVKARQLKFFEENMNF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_001193533
ORF Size	2256 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001193533.2
RefSeq ORF	2259 bp
Locus ID	6787
UniProt ID	A5YM70
Protein Families	Druggable Genome, Protein Kinase
MW	84.8 kDa
Gene Summary	The protein encoded by this gene is a serine/threonine protein kinase required for normal entry into replicative senescence. The encoded protein also is involved in cell cycle arrest in response to double-stranded DNA damage. Finally, this protein plays a role in maintaining cilium integrity, and defects in this gene have been associated with ciliopathies. [provided by RefSeq, Jan 2017]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC231401L3	Lenti-ORF clone of NEK4 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)-related kinase 4 (NEK4), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,410.00
RC231401L4	Lenti-ORF clone of NEK4 (mGFP-tagged)-Human NIMA (never in mitosis gene a)-related kinase 4 (NEK4), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,410.00
RG231401	NEK4 (tGFP-tagged) - Human NIMA (never in mitosis gene a)-related kinase 4 (NEK4), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,270.00

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