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RbAp46 (RBBP7) (NM_001198719) Human Tagged ORF Clone
CAT＃: RC231256
RBBP7 (Myc-DDK-tagged)-Human retinoblastoma binding protein 7 (RBBP7), transcript variant 1
ORF Plasmid: tGFP
"NM_001198719" in other vectors (3)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RbAp46 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC231256 representing NM_001198719
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCGAGGCGGGCGTCGTGGGAGCTGGGGCCTCCCCTGATGGGGATTGGAGAGACCAGGCCTGTG GGCTTCTGCTACACGTGCATTTGTCTTCCCGACTGGGTCGCGCAGCCCCTGTACGTACAGGTCGTCATCT TAGAACAGTGTTTGAAGATACTGTGGAGGAGCGTGTCATCAATGAAGAATATAAAATCTGGAAGAAGAAT ACACCGTTTCTATATGACCTGGTTATGACCCATGCTCTTCAGTGGCCCAGTCTTACCGTTCAGTGGCTTC CTGAAGTGACTAAACCTGAAGGAAAAGATTATGCCCTTCATTGGCTAGTGCTGGGGACTCATACGTCTGA TGAGCAGAATCATCTGGTGGTTGCTCGAGTACATATTCCCAATGATGATGCACAGTTTGATGCTTCCCAT TGTGACAGTGACAAGGGTGAATTTGGTGGCTTTGGTTCTGTAACAGGAAAAATTGAATGTGAAATTAAAA TCAATCACGAAGGAGAAGTAAACCGTGCTCGTTACATGCCGCAGAATCCTCACATCATTGCTACAAAAAC ACCATCTTCTGATGTGTTGGTTTTTGACTATACAAAACACCCTGCTAAACCAGACCCAAGTGGAGAATGT AATCCTGATCTCAGATTAAGAGGTCACCAGAAGGAAGGCTATGGTCTCTCCTGGAATTCAAATTTGAGTG GACATCTCCTAAGTGCATCTGATGACCATACTGTTTGTCTGTGGGATATAAACGCAGGACCAAAAGAAGG CAAAATTGTGGATGCTAAAGCCATCTTTACTGGCCACTCAGCTGTTGTAGAGGATGTGGCCTGGCACCTG CTGCACGAGTCATTGTTTGGATCTGTTGCTGATGATCAGAAACTTATGATATGGGACACCAGGTCCAATA CCACCTCCAAGCCGAGTCACTTGGTGGATGCGCACACTGCCGAAGTCAACTGCCTCTCATTCAATCCCTA CAGCGAATTTATTCTAGCCACCGGCTCTGCGGATAAGACCGTAGCTTTATGGGATCTGCGTAACTTAAAA TTAAAACTCCATACCTTCGAATCTCATAAAGATGAAATTTTCCAGGTCCACTGGTCTCCACATAATGAAA CTATTCTGGCTTCAAGTGGTACTGACCGCCGCCTGAATGTGTGGGATTTAAGTAAAATTGGGGAAGAACA ATCAGCAGAAGATGCAGAAGATGGGCCTCCAGAACTCCTGTTTATTCATGGAGGACACACTGCTAAGATT TCAGATTTTAGCTGGAACCCCAATGAGCCTTGGGTCATTTGCTCAGTGTCTGAGGATAACATCATGCAGA TATGGCAAATGGCTGAAAATATTTACAATGATGAAGAGTCAGATGTCACGACATCCGAACTGGAGGGACA AGGATCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC231256 representing NM_001198719
Red=Cloning site Green=Tags(s) MAAEAGVVGAGASPDGDWRDQACGLLLHVHLSSRLGRAAPVRTGRHLRTVFEDTVEERVINEEYKIWKKN TPFLYDLVMTHALQWPSLTVQWLPEVTKPEGKDYALHWLVLGTHTSDEQNHLVVARVHIPNDDAQFDASH CDSDKGEFGGFGSVTGKIECEIKINHEGEVNRARYMPQNPHIIATKTPSSDVLVFDYTKHPAKPDPSGEC NPDLRLRGHQKEGYGLSWNSNLSGHLLSASDDHTVCLWDINAGPKEGKIVDAKAIFTGHSAVVEDVAWHL LHESLFGSVADDQKLMIWDTRSNTTSKPSHLVDAHTAEVNCLSFNPYSEFILATGSADKTVALWDLRNLK LKLHTFESHKDEIFQVHWSPHNETILASSGTDRRLNVWDLSKIGEEQSAEDAEDGPPELLFIHGGHTAKI SDFSWNPNEPWVICSVSEDNIMQIWQMAENIYNDEESDVTTSELEGQGS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001198719 |
| ORF Size | 1407 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001198719.2 |
| RefSeq ORF | 1410 bp |
| Locus ID | 5931 |
| UniProt ID | Q16576 |
| Protein Families | Druggable Genome |
| MW | 52.8 kDa |
| Gene Summary | This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The RepID-CRL4 ubiquitin ligase complex regulates metaphase to anaphase transition via BUB3 degradation
,Jang, SM;Nathans, JF;Fu, H;Redon, CE;Jenkins, LM;Thakur, BL;Pongor, LS;Baris, AM;Gross, JM;OʹNeill, MJ;Indig, FE;Cappell, SD;Aladjem, MI;,
Nat Commun
,PubMed ID 31911655
[RbAp46]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC231256L3 | Lenti-ORF clone of RBBP7 (Myc-DDK-tagged)-Human retinoblastoma binding protein 7 (RBBP7), transcript variant 1 |
CNY 5,890.00 |
|
| RC231256L4 | Lenti-ORF clone of RBBP7 (mGFP-tagged)-Human retinoblastoma binding protein 7 (RBBP7), transcript variant 1 |
CNY 5,890.00 |
|
| RG231256 | RBBP7 (tGFP-tagged) - Human retinoblastoma binding protein 7 (RBBP7), transcript variant 1 |
CNY 4,370.00 |
