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Myeloid leukemia factor 1 (MLF1) (NM_001195432) Human Tagged ORF Clone
CAT＃: RC231143
MLF1 (Myc-DDK-tagged)-Human myeloid leukemia factor 1 (MLF1), transcript variant 4
ORF Plasmid: tGFP
"NM_001195432" in other vectors (3)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC231143 representing NM_001195432
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAAAAGAAGTTCTTCAGAGAGAAGGAAAATCATACAAGTCAGAAACTCTGATGTACATTAAAAAAG CAAGAGCATCAGAGAATAAGTTAAGTGAGTCCATTCTTGCACACCGAGAAAATATGCGACAGATGATAAG AAGTTTTTCTGAACCCTTTGGAAGAGACTTGCTCAGTATCTCTGATGGTAGAGGGAGAGCTCATAATCGT AGAGGACATAATGATGGTGAAGATTCTTTGACTGCAACGAGTTGTTCTCTTGTGCCTTTTGGCGATTTTG GTGGTATGCATACAGATGTCAGCTCTTTCCAGACAATGGACCAAATGGTGTCAAATATGAGAAACTATAT GCAGAAATTAGAAAGAAACTTCGGTCAACTTTCAGTGGATCCAAATGGACATTCATTTTGTTCTTCCTCA GTTATGACTTATTCCAAAATAGGAGATGAACCGCCAAAGGTTTTTCAGGCCTCAACTCAAACTCGTCGAG CTCCAGGAGGAATAAAGGAAACCAGGAAAGCAATGAGAGATTCTGACAGTGGACTAGAAAAAATGGCTAT TGGTCATCATATCCATGACCGAGCTCATGTCATTAAAAAGTCAAAGAACAAGAAGACTGGAGATGAAGAG GTCAACCAGGAGTTCATCAATATGAATGAAAGTGATGCTCATGCTTTTGATGAGGAGTGGCAAAGTGAGG TTTTGAAGTACAAACCAGGACGACACAATCTAGGAAACACTAGAATGAGAAGTGTTGGCCATGAGAATCC TGGCTCCCGAGAACTTAAAAGAAGGGAGAAACCTCAACAAAGTCCAGCCATTGAACATGGAAGGAGATCA AATGTTTTGGGGGACAAACTCCACATCAAAGGCTCATCTGTGAAAAGCAACAAAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC231143 representing NM_001195432
Red=Cloning site Green=Tags(s) MLKEVLQREGKSYKSETLMYIKKARASENKLSESILAHRENMRQMIRSFSEPFGRDLLSISDGRGRAHNR RGHNDGEDSLTATSCSLVPFGDFGGMHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSS VMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEE VNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRS NVLGDKLHIKGSSVKSNKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001195432 |
| ORF Size | 897 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001195432.2 |
| RefSeq ORF | 777 bp |
| Locus ID | 4291 |
| UniProt ID | P58340 |
| Protein Families | Druggable Genome |
| MW | 34.3 kDa |
| Gene Summary | This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC231143L3 | Lenti ORF clone of Human myeloid leukemia factor 1 (MLF1), transcript variant 4, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC231143L4 | Lenti ORF clone of Human myeloid leukemia factor 1 (MLF1), transcript variant 4, mGFP tagged |
CNY 5,890.00 |
|
| RG231143 | MLF1 (tGFP-tagged) - Human myeloid leukemia factor 1 (MLF1), transcript variant 4 |
CNY 4,370.00 |
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