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C4orf21 (ZGRF1) (NM_018392) Human Tagged ORF Clone
CAT＃: RC229463
- TrueORF®
C4orf21 (Myc-DDK-tagged)-Human chromosome 4 open reading frame 21 (C4orf21)
ORF Plasmid: tGFP
"NM_018392" in other vectors (4)
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CNY 20,832.00
| Cited in 3 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C4orf21 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC229463 representing NM_018392
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAGCCAAGAATTTATTGTTCTATATACTCATCAAAAGATGAAGAAGTCAAAAGTGTGGCAAGATG GAATTCTGAAGATCACTCACTTAGGAAACAAAGCAATTTTATATGATGACAAAGGAGCATGTTTGGAGAG TCTGTTTCTTAAATGCCTTGAGGTGAAACCTGGAGATGACTTAGAAAGTGATCGATACTTAATCACAGTT GAAGAGGTTAAAGTTGCTGGAGCCATAGGTATTGTTAAGCAGAATGTCAATAAAGAAGCACCAGAGTTAA ATTCAAGGACATTTATATCCTCTGGCCGATCTCTTGGATGTCAGCCCTCTGGCTTAAAAAGGAAGTTTAC TGGTTTTCAAGGACCACGTCAGGTTCCAAAGAAAATGGTTATTATGGAAAGTGGTGAATCAGCTGCATCA CATGAGGCTAAGAAAACTGGCCCTACTATTTTTTCTCCATTCTGCAGCATGCCTCCTTTGTTTCCTACTG TTGGCAAGAAAGATGTAAATAATATACTGGCAGACCCTGAGAACATTGTGACTTACAAGAACAGGGAGAG AAATGCCATGGATTTTTCTTCGGTTTTTTCTCCATCCTTCCAGATTAACCCAGAAGTGCTGTGTGAAGAA AATTATTTTTGCTCACCTGTCAATTCTGGAAATAAGCTTTCAGACTCTTTACTGACCAATGAGCCTGTGA AAAGAGATAGTTTGGCATCTCACTATTCAGGAGTTTCACAAAACATCAGAAGCAAAGCACAGATATTAGC TCTTCTGAAGTCCGAATCATCTAGTTCATGTGAGGAACTAAATTCTGAGATGACAGAGCATTTTCCTCAA AAACAACCACAAGGAAGTTTAAAAATTGCTACTAAACCAAAGTACCTAATTCAACAGGAAGAGTGTGCTG AGATGAAGAGCACAGAAAATTTATACTACCAGCATCAATCAGAAAATACCATGAGAAATAAAAGCCGGTG GGCCATGTATTTATCCTCACAGAGTTCACCTATACATTCTTCTACTGTAGATGGGAATGATACAGAAAGG AAACCCAAGGCCCAGGAAGATGATGTAAATTCTAATTTGAAAGACCTTTCATTACAAAAAATTATACAGT TCGTTGAAACGTATGCTGAAGAGAGGAAAAAGTATAATGTAGACCAGTCAGTCGGTAATAATGATCCATC CTGGAATCAGGAAGTAAAATTAGAAATTCCTTCATTCAATGAAAGCAGTAGCTTACAGGTTACTTGTAGC AGTGCAGAAAATGATGGTATATTATCAGAATCTGACATTCAAGAAGATAATAAAATACCTTTTAATCAAA ATGACAAGGGGTGCATTAAAGGATCAGTTCTCATTAAAGAAAATGCTCAGGAGGTAAATACATGTGGAAC ACTGGAAAAGGAGTATGAACAATCAGAGTCATCTCTGCCAGAACTGAAACATCTCCAAATTGAATCTAGT AATAATTCTAGGATCTCTGATGACATTACAGACATGATTTCTGAAAGTAAAATGGATAATGAAAGTCTTA ATAGTATTCATGAATCTCTGAGTAATGTAACACAACCATTTTTGGAGGTAACTTTTAATCTGAACAATTT TGAGACCAGTGACACTGAGGAGGAATCACAGGAAAGCAACAAAATTTCCCAGGATTCAGAGAGTTGGGTA AAGGACATTTTGGTTAATGATGGCAATTCATGTTTTCAAAAGAGGTCTGAGAATACAAACTGTGAAGAGA TTGAGGGTGAACATTTGCCATTCTTAACATCTGTTAGTGACAAACCTACAGTGACATTTCCTGTTAAAGA GACTCTGCCATCACAGTTTTGTGATAAAACTTATGTGGGTTTTGACATGGGAATATGTAAAACTGAAAAC ACAGGAAAAGAAATAGAGGAGTATAGTGACACATTAAGCAATTTTGAATCTTTCAAGTGGACTGATGCTG TATACGGAGATAATAAAGAAGATGCTAATAAACCTATTCAAGAAGTCAGAATTAACTATGATTTTGCTTT ACCCCCGAATAAATCTAAAGGTATAAACATGAATTTACATATTCCTCATATTCAAAACCAGATTGCTGAA AACAGTAATCTATTTTCAGAAGATGCTCAACCTCAGCCTTTTATTTTGGGAAGTGACTTAGACAAAAATG ATGAACATGTTTTACCCTCAACTTCTAGTAGTGACAACAGTGTCCAACTATTAAATACCAATCAGAATCA CTATGAATGTATTGCACTTGATAAATCAAATACCCACATTTCCAATTCTTTGTTTTACCCACTGGGAAAA AAGCATCTTATTTCCAAAGACACAGAAGCACATATATCTGAACCTGAAGATTTGGGAAAGATTAGAAGTC CACCCCCTGACCATGTTGAGGTTGAAACTGCCAGAGAAGGCAAACAATACTGGAATCCTAGAAATTCTTC AGAACTTTCTGGATTAGTAAATACCATTTCTATTTTAAAGTCGCTATGTGAACACAGTACTGCTTTAGAC AGCTTGGAAATATTGAAAAAGAAAAATACTGTCTTTCAGCAAGGAACTCAACAGACGTATGAGCCAGATA GCCCTCCTGAAGTGAGGAAACCATTTATTACAGTAGTTTCACCAAAGTCTCCTCATCTGCACAAGGATTC TCAACAGATACTAAAAGAAGATGAAGTTGAACTAAGTGAACCACTTCAGTCTGTGCAGTTCTCTTCCTCG GGAAGTAAAGAAGAGACTGCTTTTCAAGCTGTTATTCCTAAACAAATAGAGAGAAAAACCTGTGACCCTA AGCCTGTTGAGTTTCAAGGACATCAAGTAAAAGGATCAGCTACTAGTGGTGTAATGGTCAGAGGACACAG CTCACAGCTAGGATGCAGTCAGTTTCCAGATAGCACTGAGTATGAAAACTTTATGACAGAGACACCAGAG TTACCTAGCACGTGTATGCAGATTGACTTCTTGCAGGTGACATCACCAGAAGAAAACATCTCTACATTGA GCCCTGTTTCTACCTTTTCTTTGAACTCAAGAGATGAAGACTTCATGGTAGAATTCTCTGAGACGTCCCT GAAAGCAAGAACTTTACCTGATGATCTTCATTTTCTCAACTTGGAGGGGATGAAAAAATCCCGTTCTCTG GAGAATGAGAACCTTCAAAGGCTTTCATTATTAAGTAGAACCCAGGTTCCACTTATTACTTTGCCACGTA CTGATGGGCCACCTGACTTAGACTCTCATTCGTATATGATCAACTCTAACACATACGAGTCTTCTGGCTC CCCCATGCTCAATTTGTGTGAAAAGTCAGCAGTTCTTTCGTTTAGCATTGAGCCTGAGGACCAAAATGAA ACCTTTTTCTCTGAAGAATCTAGGGAAGTGAATCCAGGGGATGTTTCACTTAATAATATATCTACTCAGA GCAAGTGGCTGAAATATCAAAACACATCCCAATGCAACGTGGCTACTCCAAACAGAGTTGATAAGAGAAT AACTGATGGCTTCTTTGCTGAGGCTGTTTCTGGGATGCATTTTAGAGACACAAGTGAAAGACAGAGTGAT GCTGTCAATGAAAGCTCTTTAGACTCTGTGCATTTGCAAATGATAAAAGGCATGCTCTATCAACAGCGGC AGGATTTTAGCAGTCAAGATTCGGTTTCCAGAAAGAAAGTACTTTCTCTGAATTTAAAGCAGACTTCTAA GACAGAGGAAATTAAAAATGTATTAGGAGGGTCTACCTGCTACAACTACAGTGTAAAGGATTTACAGGAG ATAAGTGGCTCTGAGCTGTGCTTTCCAAGTGGGCAGAAAATAAAATCTGCTTATCTTCCCCAAAGGCAAA TTCACATACCAGCTGTTTTTCAGTCTCCTGCTCATTATAAGCAGACTTTCACATCTTGCCTCATAGAACA TCTAAATATATTGCTGTTTGGGTTAGCACAAAACCTGCAGAAAGCTCTTTCAAAAGTTGACATATCATTT TATACATCATTGAAGGGAGAGAAACTGAAAAACGCAGAAAATAATGTACCATCCTGCCATCATAGTCAAC CTGCAAAACTTGTCATGGTTAAAAAGGAAGGTCCAAATAAGGGTCGTCTCTTTTATACATGTGATGGACC CAAAGCTGATCGATGTAAATTCTTTAAATGGCTTGAGGACGTGACTCCAGGATATTCAACACAGGAAGGA GCTCGACCTGGCATGGTTTTAAGTGATATTAAGAGTATTGGCTTATATTTAAGAAGTCAAAAGATACCAC TTTATGAGGAATGCCAGCTTTTGGTGAGAAAAGGATTTGATTTTCAGAGAAAACAGTATGGCAAACTAAA GAAGTTTACTACTGTAAATCCTGAGTTTTATAATGAACCAAAAACCAAACTTTATCTTAAGCTAAGTCGG AAGGAAAGATCTTCAGCTTATAGCAAAAATGATCTTTGGGTGGTTTCAAAAACCCTAGACTTTGAGCTGG ATACTTTTATCGCATGTAGTGCTTTCTTTGGACCATCATCTATCAATGAGATAGAAATACTGCCTTTGAA AGGCTATTTCCCTTCTAATTGGCCCACTAACATGGTTGTCCATGCGTTATTGGTTTGTAATGCTAGCACA GAACTGACTACTTTGAAAAACATTCAGGACTACTTTAATCCAGCTACTCTACCTCTAACACAGTACCTGT TAACAACGTCTTCGCCAACTATAGTTAGTAACAAAAGAGTCAGTAAGAGAAAATTTATCCCACCAGCCTT CACAAATGTCAGTACAAAATTTGAACTACTCAGCCTAGGAGCAACATTGAAGTTAGCTAGTGAGTTGATT CAGGTACACAAGTTAAACAAGGATCAAGCTACAGCTCTAATTCAAATAGCTCAAATGATGGCATCACATG AAAGCATTGAAGAAGTGAAGGAACTGCAAACTCATACCTTCCCTATCACAATCATACATGGTGTGTTTGG AGCAGGAAAGAGTTACTTGCTGGCAGTGGTGATTTTGTTCTTTGTACAGCTGTTTGAAAAGAGTGAAGCT CCCACCATTGGAAATGCAAGGCCGTGGAAACTTCTGATTTCTTCTTCTACTAATGTGGCTGTTGACAGAG TACTTCTTGGGCTTCTCAGTCTTGGATTTGAAAACTTTATCAGAGTTGGGAGTGTTAGGAAGATTGCCAA ACCAATTTTACCTTATAGCTTGCATGCTGGCTCAGAAAATGAAAGTGAACAGTTAAAAGAACTACATGCA CTAATGAAAGAAGACCTGACTCCTACGGAAAGAGTCTATGTGAGAAAAAGCATTGAGCAGCATAAACTGG GGACCAATAGAACCCTGCTGAAGCAGGTTCGAGTAGTTGGAGTTACCTGTGCAGCCTGCCCATTCCCATG CATGAATGATCTTAAATTTCCTGTAGTTGTGCTGGATGAGTGTAGTCAGATAACTGAACCGGCCTCTCTC CTTCCCATTGCAAGGTTTGAGTGTGAAAAGCTGATTCTTGTTGGGGATCCCAAACAGCTACCTCCTACTA TTCAGGGTTCTGATGCAGCTCATGAAAATGGATTGGAACAAACTCTTTTTGATCGACTTTGCTTAATGGG TCACAAGCCAATTCTATTGAGAACTCAATACCGTTGTCATCCTGCAATCAGTGCTATTGCTAATGATCTG TTTTACAAAGGAGCCCTCATGAATGGTGTAACAGAAATAGAGCGGAGCCCTTTATTGGAATGGCTACCAA CCCTGTGTTTTTATAATGTTAAAGGACTAGAACAGATAGAAAGAGATAACAGCTTTCATAATGTGGCAGA AGCTACGTTTACACTCAAGCTGATTCAATCACTGATTGCAAGTGGAATAGCAGGCTCTATGATTGGTGTG ATAACATTATACAAATCCCAGATGTACAAGCTTTGTCATTTACTCAGTGCTGTGGACTTTCACCATCCTG ATATTAAAACTGTGCAGGTGTCCACAGTAGATGCTTTTCAGGGAGCTGAAAAGGAGATCATTATTCTGTC CTGTGTAAGGACAAGACAAGTAGGATTCATTGATTCAGAAAAAAGAATGAATGTTGCATTGACTAGAGGA AAGAGGCATTTGTTGATTGTGGGAAATTTAGCCTGTTTGAGGAAAAATCAACTTTGGGGACGAGTGATCC AACACTGCGAAGGAAGGGAAGATGGATTGCAACATGCAAACCAGTATGAACCACAGCTGAACCATCTCCT TAAAGATTATTTTGAAAAACAAGTGGAAGAAAAACAGAAGAAAAAGAGTGAAAAAGAGAAATCTAAAGAT AAATCTCATTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC229463 representing NM_018392
Red=Cloning site Green=Tags(s) MESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGACLESLFLKCLEVKPGDDLESDRYLITV EEVKVAGAIGIVKQNVNKEAPELNSRTFISSGRSLGCQPSGLKRKFTGFQGPRQVPKKMVIMESGESAAS HEAKKTGPTIFSPFCSMPPLFPTVGKKDVNNILADPENIVTYKNRERNAMDFSSVFSPSFQINPEVLCEE NYFCSPVNSGNKLSDSLLTNEPVKRDSLASHYSGVSQNIRSKAQILALLKSESSSSCEELNSEMTEHFPQ KQPQGSLKIATKPKYLIQQEECAEMKSTENLYYQHQSENTMRNKSRWAMYLSSQSSPIHSSTVDGNDTER KPKAQEDDVNSNLKDLSLQKIIQFVETYAEERKKYNVDQSVGNNDPSWNQEVKLEIPSFNESSSLQVTCS SAENDGILSESDIQEDNKIPFNQNDKGCIKGSVLIKENAQEVNTCGTLEKEYEQSESSLPELKHLQIESS NNSRISDDITDMISESKMDNESLNSIHESLSNVTQPFLEVTFNLNNFETSDTEEESQESNKISQDSESWV KDILVNDGNSCFQKRSENTNCEEIEGEHLPFLTSVSDKPTVTFPVKETLPSQFCDKTYVGFDMGICKTEN TGKEIEEYSDTLSNFESFKWTDAVYGDNKEDANKPIQEVRINYDFALPPNKSKGINMNLHIPHIQNQIAE NSNLFSEDAQPQPFILGSDLDKNDEHVLPSTSSSDNSVQLLNTNQNHYECIALDKSNTHISNSLFYPLGK KHLISKDTEAHISEPEDLGKIRSPPPDHVEVETAREGKQYWNPRNSSELSGLVNTISILKSLCEHSTALD SLEILKKKNTVFQQGTQQTYEPDSPPEVRKPFITVVSPKSPHLHKDSQQILKEDEVELSEPLQSVQFSSS GSKEETAFQAVIPKQIERKTCDPKPVEFQGHQVKGSATSGVMVRGHSSQLGCSQFPDSTEYENFMTETPE LPSTCMQIDFLQVTSPEENISTLSPVSTFSLNSRDEDFMVEFSETSLKARTLPDDLHFLNLEGMKKSRSL ENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQNE TFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSD AVNESSLDSVHLQMIKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQE ISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISF YTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEG ARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSR KERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNAST ELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELI QVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEA PTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHA LMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASL LPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDL FYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGV ITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRG KRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKD KSHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018392 |
| ORF Size | 6312 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018392.5 |
| RefSeq Size | 6740 bp |
| RefSeq ORF | 6315 bp |
| Locus ID | 55345 |
| UniProt ID | Q86YA3 |
| MW | 236.6 kDa |
| Gene Summary | The encoded protein contains GRF zinc finger (zf-GRF) and transmembrane domains. GRF zinc fingers are found in a number of DNA-binding proteins. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2017] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Protocol for Purification of Human ZGRF1 and Its Regulatory Function on RAD51-Mediated D-Loop Formation
,Lisby, M;Xue, X;,
STAR Protocols
,PubMed ID 32984852
[ZGRF1]
|
|
The ZGRF1 Helicase Promotes Recombinational Repair of Replication-Blocking DNA Damage in Human Cells
,Brannvoll, A;Xue, X;Kwon, Y;Kompocholi, S;Simonsen, AKW;Viswalingam, KS;Gonzalez, L;Hickson, ID;Oestergaard, VH;Mankouri, HW;Sung, P;Lisby, M;,
Cell Rep
,PubMed ID 32640219
[ZGRF1]
|
|
Gene variants in ZGRF1 implicated for a rare sensory reflex epilepsy
,Choudhury, SR;Satishchandra, P;Sinha, S;Anand, A;,
bioRxiv
[ZGRF1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229463L1 | Lenti ORF clone of Human chromosome 4 open reading frame 21 (C4orf21), Myc-DDK-tagged |
CNY 18,150.00 |
|
| RG229463 | C4orf21 (tGFP-tagged) - Human chromosome 4 open reading frame 21 (C4orf21) |
CNY 22,432.00 |
|
| SC100457 | C4orf21 (untagged)-Human chromosome 4 open reading frame 21 (C4orf21) |
CNY 40,470.00 |
|
| SC328098 | C4orf21 (untagged)-Human chromosome 4 open reading frame 21 (C4orf21) |
CNY 40,470.00 |
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