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NTE (PNPLA6) (NM_001166114) Human Tagged ORF Clone
CAT＃: RC228676
- TrueORF®
PNPLA6 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 6 (PNPLA6), transcript variant 5
ORF Plasmid: tGFP
"NM_001166114" in other vectors (4)
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CNY 13,590.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS; SPG39; sws |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC228676 representing NM_001166114
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACATCGAGTCACGGGCTGGCTACGAACTCCTCGGGGGCGAAGGTGGCGGAGAGGGATGGGTTCC AGGACGTCCTGGCGCCCGGGGAAGGCTCGGCGGGACGGATTTGCGGTGCGCAGCCAGTGCCGTTCGTCCC TCAGGTGCTTGGCGTGATGATCGGGGCCGGAGTGGCGGTGGTGGTCACGGCCGTGCTCATCCTCCTGGTG GTGCGGAGGCTGCGAGTGCCAAAAACCCCAGCCCCGGATGGCCCCCGGTATCGGTTCCGGAAGAGGGACA AAGTGCTCTTCTATGGCCGGAAGATTATGCGGAAGGTGTCACAATCCACCTCCTCCCTCGTGGATACCTC TGTCTCCGCCACCTCCCGGCCACGCATGAGGAAGAAACTGAAGATGCTCAACATTGCCAAGAAGATCCTG CGCATCCAGAAAGAGACGCCCACGCTGCAGCGGAAGGAGCCCCCGCCCGCAGTGCTAGAAGCTGACCTGA CCGAGGGCGACCTGGCTAACTCCCATCTGCCCTCTGAAGTGCTTTATATGCTCAAGAACGTCCGGGTGCT GGGCCACTTCGAGAAGCCACTCTTCCTGGAGCTCTGCCGCCACATGGTCTTCCAGCGGCTGGGCCAGGGT GACTACGTCTTCCGGCCGGGCCAGCCAGATGCCAGCATCTACGTGGTGCAGGACGGGCTGCTGGAGCTCT GTCTGCCAGGGCCTGACGGGAAGGAGTGTGTGGTGAAGGAAGTGGTTCCTGGGGACAGCGTCAACAGCCT TCTCAGCATCCTGGATGTCATCACCGGTCACCAGCATCCCCAGCGGACCGTGTCTGCCCGGGCGGCCCGG GACTCCACGGTGCTGCGCCTGCCGGTGGAAGCATTCTCCGCGGTCTTCACCAAGTACCCGGAGAGCTTGG TGCGGGTCGTGCAGATCATCATGGTGCGGCTGCAGCGAGTCACCTTCCTGGCACTGCACAACTACCTGGG TCTGACCAATGAGCTCTTCAGCCACGAGATCCAGCCCCTGCGTCTGTTCCCCAGCCCCGGCCTCCCAACT CGCACCAGCCCTGTGCGGGGCTCCAAGAGAATGGTCAGCACCTCAGCTACAGACGAGCCCAGGGAGACCC CAGGGCGGCCACCCGATCCCACCGGGGCCCCGCTGCCTGGACCTACAGGGGACCCTGTGAAGCCCACATC CCTGGAAACCCCCTCGGCCCCTCTGCTGAGCCGCTGCGTCTCCATGCCAGGGGACATCTCAGGCTTGCAG GGTGGCCCCCGCTCCGACTTCGACATGGCCTATGAGCGTGGCCGGATCTCCGTGTCCCTGCAGGAAGAGG CCTCCGGGGGGTCCCTGGCAGCCCCCGCTCGGACCCCCACTCAGGAGCCTCGTGAGCAGCCGGCAGGCGC CTGTGAATACAGCTACTGTGAGGATGAGTCGGCCACTGGTGGCTGCCCTTTCGGGCCCTACCAGGGCCGC CAGACCAGCAGCATCTTCGAGGCAGCAAAGCAGGAGCTGGCCAAGCTGATGCGGATTGAGGACCCCTCCC TCCTGAACAGCAGAGTCTTGCTGCACCACGCCAAAGCTGGCACCATCATTGCCCGCCAGGGAGACCAGGA CGTGAGCCTGCACTTCGTGCTCTGGGGCTGCCTGCACGTGTACCAGCGCATGATCGACAAGGCGGAGGAC GTGTGCCTGTTCGTAGCGCAGCCCGGGGAACTGGTGGGGCAGCTGGCGGTGCTCACTGGCGAACCTCTCA TCTTCACACTGCGAGCCCAACGCGACTGCACCTTCCTGCGGATCTCCAAGTCCGACTTCTATGAGATCAT GCGCGCACAGCCCAGTGTGGTGCTGAGTGCGGCGCACACGGTGGCAGCCAGGATGTCGCCCTTCGTGCGC CAGATGGACTTCGCCATCGACTGGACTGCAGTGGAGGCGGGACGCGCGCTGTACAGGCAGGGCGACCGCT CCGACTGCACTTACATCGTGCTCAATGGGCGGCTGCGTAGCGTGATCCAGCGAGGCAGTGGCAAGAAGGA GCTGGTGGGCGAGTACGGCCGCGGCGACCTCATCGGCGTGGTGGAGGCACTGACCCGGCAGCCGCGAGCC ACGACGGTGCACGCGGTGCGCGACACGGAGCTGGCCAAGCTTCCCGAGGGCACCTTGGGTCACATCAAAC GCCGGTACCCGCAGGTCGTGACCCGCCTTATCCACCTACTGAGCCAGAAAATTCTAGGGAATTTGCAGCA GCTGCAAGGACCCTTCCCAGGCTCTGGGTTGGGTGTGCCCCCACACTCGGAACTCACCAACCCAGCCAGC AACCTGGCAACTGTGGCAATCCTGCCTGTGTGTGCTGAGGTCCCCATGGTGGCCTTCACGCTGGAGCTGC AGCACGCCCTGCAGGCCATCGGTCCGACGCTACTCCTTAACAGTGACATCATCCGGGCACGCCTGGGGGC CTCCGCACTGGATAGCATCCAAGAGTTCCGGCTGTCAGGGTGGCTGGCCCAGCAGGAGGATGCACACCGT ATCGTACTCTACCAGACGGACGCCTCGCTGACGCCCTGGACCGTGCGCTGCCTGCGACAGGCCGACTGCA TCCTCATTGTGGGCCTGGGGGACCAGGAGCCTACCCTCGGCCAGCTGGAGCAGATGCTGGAGAACACGGC TGTGCGCGCCCTTAAGCAGCTAGTCCTGCTCCACCGAGAGGAGGGCGCGGGCCCCACGCGCACCGTGGAG TGGCTAAATATGCGCAGCTGGTGCTCGGGGCACCTGCACCTGCGCTGTCCGCGCCGCCTCTTTTCGCGCC GCAGCCCTGCCAAGCTGCATGAGCTCTACGAGAAGGTTTTCTCCAGGCGCGCGGACCGGCACAGCGACTT CTCCCGCTTGGCGAGGGTGCTCACGGGGAACACCATTGCCCTTGTGCTAGGCGGGGGCGGGGCCAGGGGC TGCTCGCACATCGGAGTACTAAAGGCATTAGAGGAGGCGGGGGTCCCCGTGGACCTGGTGGGCGGCACGT CCATTGGCTCTTTCATCGGAGCGTTGTACGCGGAGGAGCGCAGCGCCAGCCGCACGAAGCAGCGGGCCCG GGAGTGGGCCAAGAGCATGACTTCGGTGCTGGAACCTGTGTTGGACCTCACGTACCCAGTCACCTCCATG TTCACTGGGTCTGCCTTTAACCGCAGCATCCATCGGGTCTTCCAGGATAAGCAGATTGAGGACCTGTGGC TGCCTTACTTCAACGTGACCACAGATATCACCGCCTCAGCCATGCGAGTCCACAAAGATGGCTCCCTGTG GCGGTACGTGCGCGCCAGCATGACGCTGTCGGGCTACCTGCCCCCGCTGTGCGACCCCAAGGACGGGCAC CTACTCATGGATGGCGGCTACATCAACAATCTGCCAGCGGACATCGCCCGCAGCATGGGTGCCAAAACGG TCATCGCCATTGACGTGGGGAGCCAGGATGAGACGGACCTCAGCACCTACGGGGACAGCCTGTCCGGCTG GTGGCTGCTGTGGAAGCGGCTGAATCCCTGGGCTGACAAGGTAAAGGTTCCAGACATGGCTGAAATCCAG TCCCGCCTGGCCTACGTGTCCTGTGTGCGGCAGCTAGAGGTTGTCAAGTCCAGCTCCTACTGCGAGTACC TGCGCCCGCCCATCGACTGCTTCAAGACCATGGACTTTGGGAAGTTCGACCAGATCTATGATGTGGGCTA CCAGTACGGGAAGGCGGTGTTTGGAGGCTGGAGCCGTGGCAACGTCATTGAGAAAATGCTCACAGACCGG CGGTCTACAGACCTTAATGAGAGCCGCCGTGCAGACGTGCTTGCCTTCCCAAGCTCTGGCTTCACTGACT TGGCAGAGATTGTGTCCCGGATTGAGCCCCCCACGAGCTATGTCTCTGATGGCTGTGCTGACGGAGAGGA GTCAGATTGTCTGACAGAGTATGAGGAGGACGCCGGACCCGACTGCTCGAGGGATGAAGGGGGGTCCCCC GAGGGCGCAAGCCCCAGCACTGCCTCCGAGATGGAGGAGGAGAAGTCGATTCTCCGGCAACGACGCTGTC TGCCCCAGGAGCCGCCCGGCTCAGCCACAGATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228676 representing NM_001166114
Red=Cloning site Green=Tags(s) MGTSSHGLATNSSGAKVAERDGFQDVLAPGEGSAGRICGAQPVPFVPQVLGVMIGAGVAVVVTAVLILLV VRRLRVPKTPAPDGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSATSRPRMRKKLKMLNIAKKIL RIQKETPTLQRKEPPPAVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQG DYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAAR DSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPT RTSPVRGSKRMVSTSATDEPRETPGRPPDPTGAPLPGPTGDPVKPTSLETPSAPLLSRCVSMPGDISGLQ GGPRSDFDMAYERGRISVSLQEEASGGSLAAPARTPTQEPREQPAGACEYSYCEDESATGGCPFGPYQGR QTSSIFEAAKQELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAED VCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSDFYEIMRAQPSVVLSAAHTVAARMSPFVR QMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRA TTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLGVPPHSELTNPAS NLATVAILPVCAEVPMVAFTLELQHALQAIGPTLLLNSDIIRARLGASALDSIQEFRLSGWLAQQEDAHR IVLYQTDASLTPWTVRCLRQADCILIVGLGDQEPTLGQLEQMLENTAVRALKQLVLLHREEGAGPTRTVE WLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARG CSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSM FTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGH LLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQ SRLAYVSCVRQLEVVKSSSYCEYLRPPIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWSRGNVIEKMLTDR RSTDLNESRRADVLAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSP EGASPSTASEMEEEKSILRQRRCLPQEPPGSATDA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001166114 |
| ORF Size | 4095 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001166114.2 |
| RefSeq ORF | 4098 bp |
| Locus ID | 10908 |
| UniProt ID | Q8IY17 |
| Protein Families | Transmembrane |
| MW | 149.7 kDa |
| Gene Summary | This gene encodes a phospholipase that deacetylates intracellular phosphatidylcholine to produce glycerophosphocholine. It is thought to function in neurite outgrowth and process elongation during neuronal differentiation. The protein is anchored to the cytoplasmic face of the endoplasmic reticulum in both neurons and non-neuronal cells. Mutations in this gene result in autosomal recessive spastic paraplegia, and the protein is the target for neurodegeneration induced by organophosphorus compounds and chemical warfare agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228676L3 | Lenti-ORF clone of PNPLA6 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 6 (PNPLA6), transcript variant 5 |
CNY 15,490.00 |
|
| RC228676L4 | Lenti-ORF clone of PNPLA6 (mGFP-tagged)-Human patatin-like phospholipase domain containing 6 (PNPLA6), transcript variant 5 |
CNY 15,490.00 |
|
| RG228676 | PNPLA6 (tGFP-tagged) - Human patatin-like phospholipase domain containing 6 (PNPLA6), transcript variant 5 |
CNY 14,920.00 |
|
| SC327311 | PNPLA6 (untagged)-Human patatin-like phospholipase domain containing 6 (PNPLA6) transcript variant 5 |
CNY 26,220.00 |
