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Neurofascin (NFASC) (NM_001160333) Human Tagged ORF Clone
CAT＃: RC228501
NFASC (Myc-DDK-tagged)-Human neurofascin (NFASC), transcript variant 6
ORF Plasmid: tGFP
"NM_001160333" in other vectors (4)
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CNY 4,568.00
CNY 5,420.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NEDCPMD; NF; NRCAML |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC228501 representing NM_001160333
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGGCAGCCACCGCCGCCCTGGGTCCATGCAGCCTTCCTCCTCTGCCTCCTCAGTCTTGGCGGAG CCATCGAAATTCCTATGGATCTGACGCAGCCGCCAACCATCACCAAGCAGTCAGCGAAGGATCACATCGT GGACCCCCGTGATAACATCCTGATTGAGTGTGAAGCAAAAGGGAACCCTGCCCCCAGCTTCCACTGGACA CGAAACAGCAGATTCTTCAACATCGCCAAGGACCCCCGGGTGTCCATGAGGAGGAGGTCTGGGACCCTGG TGATTGACTTCCGCAGTGGCGGGCGGCCGGAGGAATATGAGGGGGAATATCAGTGCTTCGCCCGCAACAA ATTTGGCACGGCCCTGTCCAATAGGATCCGCCTGCAGGTGTCTAAATCTCCTCTGTGGCCCAAGGAAAAC CTAGACCCTGTCGTGGTCCAAGAGGGCGCTCCTTTGACGCTCCAGTGCAACCCCCCGCCTGGACTTCCAT CCCCGGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAAGACAAACGTGTCTCTCAGGGCCA TAACGGAGACCTATACTTCTCCAACGTGATGCTGCAGGACATGCAGACCGACTACAGTTGTAACGCCCGC TTCCACTTCACCCACACCATCCAGCAGAAGAACCCTTTCACCCTCAAGGTCCTCACCACCCGAGGAGTTG CAGAAAGAACACCAAGCTTCATGTATCCCCAGGGCACCGCGAGCAGCCAGATGGTGCTTCGTGGCATGGA CCTCCTGCTGGAATGCATCGCCTCCGGGGTCCCAACACCAGACATCGCATGGTACAAGAAAGGTGGGGAC CTCCCATCTGATAAGGCCAAGTTTGAGAACTTTAATAAGGCCCTGCGTATCACAAATGTCTCTGAGGAAG ACTCCGGGGAGTATTTCTGCCTGGCCTCCAACAAGATGGGCAGCATCCGGCACACGATCTCGGTGAGAGT AAAGGCTGCTCCCTACTGGCTGGACGAACCCAAGAACCTTATTCTGGCTCCTGGCGAGGATGGGAGACTG GTGTGTCGAGCCAATGGAAACCCCAAACCCACTGTCCAGTGGATGGTGAATGGGGAACCTTTGCAATCGG CACCACCTAACCCAAACCGTGAGGTGGCCGGAGACACCATCATCTTCCGGGACACCCAGATCAGCAGCAG GGCTGTGTACCAGTGCAACACCTCCAACGAGCATGGCTACCTGCTGGCCAACGCCTTTGTCAGTGTGCTG GATGTGCCGCCTCGGATGCTGTCGCCCCGGAACCAGCTCATTCGAGTGATTCTTTACAACCGGACGCGGC TGGACTGCCCTTTCTTTGGGTCTCCCATCCCCACACTGCGATGGTTTAAGAATGGGCAAGGAAGCAACCT GGATGGTGGCAACTACCATGTTTATGAGAACGGCAGTCTGGAAATTAAGATGATCCGCAAAGAGGACCAG GGCATCTACACCTGTGTCGCCACCAACATCCTGGGCAAAGCTGAAAACCAAGTCCGCCTGGAGGTCAAAG ACCCCACCAGGATCTACCGGATGCCCGAGGACCAGGTGGCCAGAAGGGGCACCACGGTGCAGCTGGAGTG TCGGGTGAAGCACGACCCCTCCCTGAAACTCACCGTCTCCTGGCTGAAGGATGACGAGCCGCTCTATATT GGAAACAGGATGAAGAAGGAAGACGACTCCCTGACCATCTTTGGGGTGGCAGAGCGGGACCAGGGCAGTT ACACGTGTGTCGCCAGCACCGAGCTAGACCAAGACCTGGCCAAGGCCTACCTCACCGTGCTAGGTAACTG CCCATGCTCACCCTGGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228501 representing NM_001160333
Red=Cloning site Green=Tags(s) MARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWT RNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKEN LDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNAR FHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGD LPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRL VCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVL DVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQ GIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYI GNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGNCPCSPWH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001160333 |
| ORF Size | 1839 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001160333.2 |
| RefSeq ORF | 1842 bp |
| Locus ID | 23114 |
| UniProt ID | O94856 |
| Protein Families | Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs) |
| MW | 68.9 kDa |
| Gene Summary | This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228501L3 | Lenti-ORF clone of NFASC (Myc-DDK-tagged)-Human neurofascin (NFASC), transcript variant 6 |
CNY 7,320.00 |
|
| RC228501L4 | Lenti-ORF clone of NFASC (mGFP-tagged)-Human neurofascin (NFASC), transcript variant 6 |
CNY 7,320.00 |
|
| RG228501 | NFASC (tGFP-tagged) - Human neurofascin (NFASC), transcript variant 6 |
CNY 5,990.00 |
|
| SC327136 | NFASC (untagged)-Human neurofascin homolog (chicken) (NFASC) transcript variant 6 |
CNY 9,880.00 |
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