beta COP (COPB1) (NM_001144062) Human Tagged ORF Clone
CAT#: RC227862
COPB1 (Myc-DDK-tagged)-Human coatomer protein complex, subunit beta 1 (COPB1), transcript variant 3
ORF Plasmid: tGFP
"NM_001144062" in other vectors (4)
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CNY 6,976.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BARMACS; COPB |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC227862 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGCGGCTGAGAACGTATGCTACACGTTAATTAACGTGCCAATGGATTCAGAACCACCATCTGAAA TTAGCTTAAAAAATGATCTAGAAAAAGGAGATGTAAAGTCAAAGACTGAAGCTTTGAAGAAAGTAATCAT TATGATTCTGAATGGTGAAAAACTTCCTGGACTTCTGATGACCATCATTCGTTTTGTGCTACCTCTTCAG GATCACACTATCAAGAAATTACTTCTGGTATTTTGGGAAATTGTTCCTAAAACAACTCCAGATGGGAGAC TTTTACATGAGATGATCCTTGTATGTGATGCATACAGAAAGGATCTTCAACATCCTAATGAATTTATTCG AGGATCTACTCTTCGTTTTCTTTGCAAATTGAAAGAAGCAGAATTGCTAGAACCTTTAATGCCAGCTATT CGTGCATGTTTGGAGCATCGACACAGCTATGTTAGAAGAAATGCTGTTTTGGCCATCTATACCATCTATA GAAATTTTGAACATCTTATACCTGATGCTCCTGAACTGATACATGATTTTCTGGTGAATGAGAAGGATGC AAGTTGCAAAAGGAATGCATTTATGATGCTAATTCATGCAGATCAGGATCGAGCTTTGGATTACTTAAGT ACTTGCATTGATCAAGTTCAAACATTTGGAGACATTCTGCAGCTGGTTATTGTTGAACTGATTTATAAGG TCTGTCATGCTAATCCATCAGAAAGAGCTCGTTTTATTCGCTGCATCTATAACTTATTACAGTCATCCAG CCCTGCTGTAAAATATGAAGCTGCTGGGACATTAGTGACACTCTCTAGTGCACCAACTGCAATCAAGGCT GCTGCTCAGTGTTACATTGATTTAATTATTAAGGAGAGCGACAACAATGTAAAACTCATAGTTTTGGATC GCTTGATAGAATTAAAAGAGCATCCTGCTCATGAACGAGTACTACAGGATCTGGTTATGGATATCCTAAG AGTATTGAGCACACCAGACTTAGAAGTACGAAAGAAAACTCTGCAGTTAGCACTGGATCTTGTCTCTTCT AGAAATGTTGAAGAGCTGGTTATTGTCCTGAAGAAGGAAGTGATAAAAACAAATAATGTGTCTGAGCATG AAGATACTGACAAATACAGACAACTCCTAGTGCGAACATTGCATTCCTGTTCTGTCCGATTTCCAGATAT GGCTGCAAATGTTATTCCTGTGTTAATGGAATTTCTCAGTGACAACAACGAAGCAGCAGCTGCTGATGTC TTGGAGTTTGTTCGTGAAGCCATTCAGCGCTTTGATAACCTGAGAATGCTTATTGTTGAGAAGATGCTTG AAGTCTTTCATGCTATTAAATCTGTCAAGATTTACCGAGGAGCATTATGGATCCTGGGAGAATACTGTAG TACCAAGGAAGACATTCAGAGTGTGATGACTGAGATCCGCAGGTCCCTTGGAGAGATCCCAATTGTAGAG TCAGAAATAAAGAAAGAAGCTGGTGAATTAAAACCTGAAGAAGAAATAACTGTAGGGCCAGTTCAGAAAT TGGTTACTGAAATGGGTACCTATGCAACTCAGAGTGCCCTTAGCAGTTCTAGACCCACCAAGAAAGAGGA AGACAGACCTCCCTTGAGAGGATTCCTTCTGGATGGAGATTTCTTTGTTGCTGCCTCCCTTGCCACAACT CTGACCAAGATTGCATTGCGCTATGTAGCTTTGGTTCAGGAGAAGAAAAAGCAAAATTCTTTTGTTGCTG AGGCTATGTTGCTCATGGCTACTATCCTGCATTTGGGAAAATCCTCTCTTCCTAAGAAGCCAATTACTGA TGATGATGTGGATCGAATTTCCCTGTGCCTCAAGGTCTTGTCTGAATGTTCACCTTTAATGAATGACATT TTCAATAAGGAATGCAGACAGTCCCTTTCTCACATGTTATCTGCTAAACTAGAAGAAGAGAAATTATCCC AAAAGAAAGAATCTGAAAAGAGGAATGTGACAGTACAGCCTGATGACCCCATTTCCTTCATGCAACTAAC TGCTAAGAATGAAATGAACTGCAAGGAAGATCAGTTTCAGCTGAGTTTACTGGCAGCAATGGGTAACACA CAGAGGAAAGAGGCAGCAGATCCCCTAGCATCTAAACTTAACAAGGTCACCCAATTGACAGGTTTCTCAG ATCCTGTATATGCAGAAGCTTACGTTCATGTCAACCAATATGATATTGTCCTGGATGTACTTGTTGTGAA CCAAACCAGTGATACTTTGCAGAATTGCACATTAGAACTAGCTACACTAGGGGATCTGAAACTTGTGGAA AAGCCGTCTCCTTTGACTCTTGCTCCTCATGACTTCGCAAATATTAAAGCTAACGTCAAAGTAGCATCAA CAGAAAATGGAATAATTTTTGGTAATATAGTTTATGATGTCTCTGGAGCAGCAAGTGACAGAAATTGTGT GGTTCTCAGTGATATTCACATCGACATCATGGACTATATCCAGCCTGCAACTTGCACTGATGCAGAATTC CGTCAGATGTGGGCCGAATTTGAATGGGAAAACAAAGTGACAGTTAACACCAACATGGTTGATTTAAATG ACTACTTACAGCACATATTAAAGTCAACCAATATGAAATGCCTGACTCCAGAAAAGGCCCTTTCTGGTTA CTGTGGCTTTATGGCAGCCAACCTTTATGCTCGTTCCATATTTGGTGAAGATGCACTTGCAAATGTCAGC ATTGAGAAGCCAATTCACCAGGGACCAGATGCTGCTGTTACCGGCCATATAAGAATTCGTGCAAAGAGCC AGGGAATGGCCTTAAGTCTTGGAGATAAAATCAACTTGTCACAGAAGAAAACTAGTATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227862 protein sequence
Red=Cloning site Green=Tags(s) MTAAENVCYTLINVPMDSEPPSEISLKNDLEKGDVKSKTEALKKVIIMILNGEKLPGLLMTIIRFVLPLQ DHTIKKLLLVFWEIVPKTTPDGRLLHEMILVCDAYRKDLQHPNEFIRGSTLRFLCKLKEAELLEPLMPAI RACLEHRHSYVRRNAVLAIYTIYRNFEHLIPDAPELIHDFLVNEKDASCKRNAFMMLIHADQDRALDYLS TCIDQVQTFGDILQLVIVELIYKVCHANPSERARFIRCIYNLLQSSSPAVKYEAAGTLVTLSSAPTAIKA AAQCYIDLIIKESDNNVKLIVLDRLIELKEHPAHERVLQDLVMDILRVLSTPDLEVRKKTLQLALDLVSS RNVEELVIVLKKEVIKTNNVSEHEDTDKYRQLLVRTLHSCSVRFPDMAANVIPVLMEFLSDNNEAAAADV LEFVREAIQRFDNLRMLIVEKMLEVFHAIKSVKIYRGALWILGEYCSTKEDIQSVMTEIRRSLGEIPIVE SEIKKEAGELKPEEEITVGPVQKLVTEMGTYATQSALSSSRPTKKEEDRPPLRGFLLDGDFFVAASLATT LTKIALRYVALVQEKKKQNSFVAEAMLLMATILHLGKSSLPKKPITDDDVDRISLCLKVLSECSPLMNDI FNKECRQSLSHMLSAKLEEEKLSQKKESEKRNVTVQPDDPISFMQLTAKNEMNCKEDQFQLSLLAAMGNT QRKEAADPLASKLNKVTQLTGFSDPVYAEAYVHVNQYDIVLDVLVVNQTSDTLQNCTLELATLGDLKLVE KPSPLTLAPHDFANIKANVKVASTENGIIFGNIVYDVSGAASDRNCVVLSDIHIDIMDYIQPATCTDAEF RQMWAEFEWENKVTVNTNMVDLNDYLQHILKSTNMKCLTPEKALSGYCGFMAANLYARSIFGEDALANVS IEKPIHQGPDAAVTGHIRIRAKSQGMALSLGDKINLSQKKTSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001144062 |
ORF Size | 2859 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001144062.1, NP_001137534.1 |
RefSeq Size | 3368 bp |
RefSeq ORF | 2862 bp |
Locus ID | 1315 |
UniProt ID | P53618 |
MW | 107.1 kDa |
Gene Summary | This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227862L3 | Lenti ORF clone of Human coatomer protein complex, subunit beta 1 (COPB1), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC227862L4 | Lenti ORF clone of Human coatomer protein complex, subunit beta 1 (COPB1), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
RG227862 | COPB1 (tGFP-tagged) - Human coatomer protein complex, subunit beta 1 (COPB1), transcript variant 3 |
CNY 4,370.00 |
|
SC325378 | COPB1 (untagged)-Human coatomer protein complex, subunit beta 1 (COPB1), transcript variant 3 |
CNY 7,220.00 |
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