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OTUD5 (NM_001136157) Human Tagged ORF Clone
CAT＃: RC227573
OTUD5 (Myc-DDK-tagged)-Human OTU domain containing 5 (OTUD5), transcript variant 2
ORF Plasmid: tGFP
"NM_001136157" in other vectors (4)
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CNY 4,216.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DUBA; MCAND |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC227573 representing NM_001136157
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGACTATACTCCCCAAAAAGAAGCCGCCGCCTCCCGACGCCGACCCCGCCAACGAGCCGCCGCCGCCCG GGCCGATGCCCCCGGCGCCGCGGCGCGGCGGAGGTGTGGGCGTGGGCGGCGGCGGCACGGGCGTGGGCGG CGGCGATCGCGACCGTGACTCCGGCGTCGTGGGGGCCCGTCCGCGAGCTTCGCCACCGCCTCAAGGCCCG CTACCAGGACCGCCGGGCGCTCTTCATCGCTGGGCGCTGGCCGTGCCGCCTGGTGCAGTGGCGGGTCCCC GGCCACAACAGGCTTCTCCACCTCCTTGCGGGGGCCCAGGTGGTCCCGGCGGCGGTCCCGGCGACGCGCT GGGCGCAGCGGCGGCGGGTGTGGGTGCCGCGGGCGTGGTGGTGGGTGTGGGTGGTGCCGTAGGCGTGGGC GGCTGCTGCTCCGGGCCTGGGCACAGCAAGCGGCGACGTCAAGCTCCCGGGGTTGGCGCGGTTGGCGGGG GCAGTCCCGAGCGTGAGGAGGTCGGCGCAGGCTACAACAGTGAGGACGAGTATGAGGCGGCTGCAGCACG CATCGAGGCTATGGACCCTGCCACTGTCGAGCAGCAGGAGCATTGGTTTGAAAAGGCCCTACGAGACAAG AAGGGCTTCATCATCAAGCAGATGAAGGAGGATGGCGCCTGTCTCTTCCGGGCTGTAGCTGACCAGGTGT ATGGAGACCAGGACATGCATGAGGTTGTGCGAAAGCATTGCATGGACTATCTGATGAAGAATGCCGACTA CTTCTCCAACTATGTCACAGAGGACTTTACCACCTACATTAACAGGAAGCGGAAAAACAATTGCCATGGC AACCACATTGAGATGCAGGCCATGGCAGAGATGTACAACCGTCCTGTGGAGGTGTACCAGTACAGCACAG AACCCATCAACACATTCCATGGGATACATCAAAACGAGGACGAACCCATTCGTGTTAGCTACCATCGGAA TATCCACTATAATTCAGTGGTGAATCCTAACAAGGCCACCATTGGTGTGGGGCTGGGCCTGCCATCATTC AAACCAGGGTTTGCAGAGCAGTCTCTGATGAAGAATGCCATAAAAACATCGGAGGAGTCATGGATTGAAC AGCAGATGCTAGAAGACAAGAAACGGGCCACAGACTGGGAGGCCACAAATGAAGCCATCGAGGAGCAGGT GGCTCGGGAATCCTACCTGCAGTGGTTGCGGGATCAGGAGAAACAGGCTCGCCAGGTCCGAGGCCCCAGC CAGCCCCGGAAAGCCAGCGCCACATGCAGTTCGGCCACAGCAGCAGCCTCCAGTGGCCTGGAGGAGTGGA CTAGCCGGTCCCCGCGGCAGCGGAGTTCAGCCTCGTCACCTGAGCACCCTGAGCTGCATGCTGAATTGGG CATGAAGCCCCCTTCCCCAGGCACTGTTTTAGCTCTTGCCAAACCTCCTTCGCCCTGTGCGCCAGGTACA AGCAGTCAGTTCTCGGCAGGGGCCGACCGGGCAACTTCCCCCCTTGTGTCCCTCTACCCTGCTTTGGAGT GCCGGGCCCTCATTCAGCAGATGTCCCCCTCTGCCTTTGGTCTGAATGACTGGGATGATGATGAGATCCT AGCTTCGGTGCTGGCAGTGTCCCAACAGGAATACCTAGACAGTATGAAGAAAAACAAAGTGCACAGAGAC CCGCCCCCAGACAAGAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227573 representing NM_001136157
Red=Cloning site Green=Tags(s) MTILPKKKPPPPDADPANEPPPPGPMPPAPRRGGGVGVGGGGTGVGGGDRDRDSGVVGARPRASPPPQGP LPGPPGALHRWALAVPPGAVAGPRPQQASPPPCGGPGGPGGGPGDALGAAAAGVGAAGVVVGVGGAVGVG GCCSGPGHSKRRRQAPGVGAVGGGSPEREEVGAGYNSEDEYEAAAARIEAMDPATVEQQEHWFEKALRDK KGFIIKQMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHG NHIEMQAMAEMYNRPVEVYQYSTEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGVGLGLPSF KPGFAEQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQVARESYLQWLRDQEKQARQVRGPS QPRKASATCSSATAAASSGLEEWTSRSPRQRSSASSPEHPELHAELGMKPPSPGTVLALAKPPSPCAPGT SSQFSAGADRATSPLVSLYPALECRALIQQMSPSAFGLNDWDDDEILASVLAVSQQEYLDSMKKNKVHRD PPPDKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001136157 |
| ORF Size | 1698 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001136157.2 |
| RefSeq ORF | 1701 bp |
| Locus ID | 55593 |
| UniProt ID | Q96G74 |
| Protein Families | Protease |
| Protein Pathways | RIG-I-like receptor signaling pathway |
| MW | 60 kDa |
| Gene Summary | This gene encodes a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Oct 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227573L3 | Lenti ORF clone of Human OTU domain containing 5 (OTUD5), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC227573L4 | Lenti ORF clone of Human OTU domain containing 5 (OTUD5), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG227573 | OTUD5 (tGFP-tagged) - Human OTU domain containing 5 (OTUD5), transcript variant 2 |
CNY 5,040.00 |
|
| SC326073 | OTUD5 (untagged)-Human OTU domain containing 5 (OTUD5), transcript variant 2 |
CNY 9,120.00 |
