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NSMAF (NM_001144772) Human Tagged ORF Clone
CAT＃: RC227565
- TrueORF®
NSMAF (Myc-DDK-tagged)-Human neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), transcript variant 2
ORF Plasmid: tGFP
"NM_001144772" in other vectors (4)
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CNY 7,130.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FAN; GRAMD5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC227565 representing NM_001144772
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGGCCAGGCCAGATTCCACCGCGGAGCCGAGGCATGCCGAGCTATCCGGCAGTGCAGGTCCCGGC GGCCGCGGGCAGCTCACCCGGAAGGCCGCGCGGGGTCCGGGCGTGGATCCCGGCACGCGAGCCCGGGCGT TCGCAGGGGAGGATTTTCCTTGCTGCTGCTTAACTTGGAGGAGTACTACTTTGAACAGCATAGAGCCAAT CACATTTTGCACAAGGGCAGTCACCATGAAAGGAAAATCAGAGGCTCCTTAAAAATATGTTCAAAATCGG TGATTTTTGAACCAGATTCAATATCCCAGCCCATCATCAAGATTCCTTTGAGAGACTGTATAAAAATAGG AAAGCATGGAGAAAATGGAGCCAATAGACACTTCACAAAGGCAAAATCTGGGGGTATTTCACTCATTTTC AGTCAGGTATATTTCATTAAAGAACATAATGTTGTTGCACCATATAAAATAGAAAGGGGCAAAATGGAAT ATGTTTTTGAATTGGATGTTCCCGGGAAAGTGGAAGATGTTGTGGAGACGTTGCTTCAGCTTCACAGAGC ATCCTGCCTTGACAAATTGGGTGACCAAACCGCCATGATAACAGCTATTTTGCAGTCTCGTTTAGCTAGA ACATCATTTGACAAAAACAGGTTCCAAAACATTTCTGAAAAGCTGCACATGGAATGCAAAGCAGAAATGG TGACGCCTCTGGTGACTAATCCTGGACACGTGTGCATCACGGACACAAACCTGTATTTTCAGCCCCTCAA CGGCTACCCGAAACCTGTGGTCCAGATAACACTCCAAGATGTCCGCCGCATCTACAAAAGGAGGCACGGC CTCATGCCTCTGGGCTTGGAAGTATTTTGCACAGAAGATGATCTGTGTTCCGACATCTACCTAAAGTTCT ATGAACCTCAAGATAGAGATGATCTCTATTTTTACATTGCCACATACCTAGAGCACCATGTGGCGGAGCA CACTGCTGAGAGCTACATGCTGCAGTGGCAGCGTGGACACCTTTCCAACTATCAGTACCTCCTTCACCTC AACAACCTGGCCGACCGCAGCTGCAACGACCTCTCCCAGTACCCTGTGTTTCCATGGATAATACATGATT ATTCCAGCTCAGAACTAGATTTGTCAAATCCAGGAACCTTCCGGGATCTCAGTAAGCCAGTAGGGGCCCT AAATAAGGAACGGCTGGAGAGACTACTGACACGCTACCAGGAAATGCCTGAACCAAAGTTCATGTATGGG AGTCACTACTCTTCCCCGGGTTATGTACTTTTTTATCTTGTTAGGATTGCACCAGAGTATATGCTGTGCC TGCAGAATGGAAGATTTGATAATGCAGATAGAATGTTCAACAGTATTGCAGAAACTTGGAAAAACTGTCT GGATGGTGCAACGGATTTTAAAGAGTTAATTCCAGAATTCTATGGTGATGATGTGAGCTTTCTAGTCAAT AGCCTGAAGTTGGATTTGGGAAAGAGACAAGGAGGACAGATGGTTGACGACGTGGAGCTTCCCCCTTGGG CTTCCAGTCCCGAGGACTTTCTCCAGAAGAGCAAAGATGCATTGGAAAGCAATTATGTGTCTGAACACCT TCACGAGTGGATTGATCTAATATTTGGCTACAAACAAAAAGGGAGTGATGCAGTTGGGGCCCATAATGTA TTTCATCCCCTGACCTATGAAGGAGGTGTAGACTTGAACAGCATCCAGGATCCTGATGAGAAGGTAGCCA TGCTTACGCAAATCTTGGAATTTGGGCAGACACCAAAACAACTATTTGTGACACCACATCCTCGAAGGAT CACCCCAAAGTTTAAAAGTTTGTCCCAGACCTCCAGTTATAATGCTTCTATGGCAGATTCCCCAGGTGAA GAGTCTTTTGAAGACCTGACCGAAGAAAGCAAAACACTGGCCTGGAATAACATCACCAAACTGCAGTTAC ACGAGCACTATAAAATCCACAAAGAAGCAGTTACTGGAATCACGGTCTCTCGCAATGGATCTTCAGTATT CACAACATCCCAAGATTCCACCTTGAAGATGTTTTCTAAAGAATCAAAAATGCTACAAAGAAGTATATCA TTTTCAAATATGGCTTTATCGTCTTGTTTACTTTTACCAGGAGATGCCACTGTCATAACTTCTTCATGGG ATAATAATGTCTATTTTTATTCCATAGCATTTGGAAGACGCCAGGACACGTTAATGGGACATGATGATGC TGTTAGTAAGATCTGTTGGCATGACAACAGGCTATATTCTGCATCGTGGGACTCTACAGTGAAGGTGTGG TCTGGTGTTCCTGCAGAGATGCCAGGCACCAAAAGACACCACTTTGACTTGCTGGCCGAGCTGGAACATG ATGTCAGTGTAGATACAATCAGTTTAAATGCTGCAAGCACACTGTTAGTTTCCGGCACCAAAGAAGGCAC AGTGAATATTTGGGACCTCACAACGGCCACCTTAATGCACCAGATTCCATGCCATTCAGGGATTGTATGT GACACTGCTTTTAGCCCAGATAGTCGCCATGTCCTCAGCACAGGAACAGATGGCTGTCTTAATGTCATTG ATGTGCAGACAGGAATGCTCATCTCCTCCATGACATCAGATGAGCCCCAGAGGTGCTTTGTCTGGGATGG AAATTCCGTTTTATCTGGCAGTCAGTCTGGTGAACTGCTCGTTTGGGACCTCCTTGGAGCAAAAATCAGT GAGAGAATACAGGGCCACACAGGTGCTGTGACATGTATATGGATGAATGAACAGTGTAGCAGTATCATCA CAGGAGGGGAAGACAGACAAATTATATTCTGGAAATTGCAGTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227565 representing NM_001144772
Red=Cloning site Green=Tags(s) MTGQARFHRGAEACRAIRQCRSRRPRAAHPEGRAGSGRGSRHASPGVRRGGFSLLLLNLEEYYFEQHRAN HILHKGSHHERKIRGSLKICSKSVIFEPDSISQPIIKIPLRDCIKIGKHGENGANRHFTKAKSGGISLIF SQVYFIKEHNVVAPYKIERGKMEYVFELDVPGKVEDVVETLLQLHRASCLDKLGDQTAMITAILQSRLAR TSFDKNRFQNISEKLHMECKAEMVTPLVTNPGHVCITDTNLYFQPLNGYPKPVVQITLQDVRRIYKRRHG LMPLGLEVFCTEDDLCSDIYLKFYEPQDRDDLYFYIATYLEHHVAEHTAESYMLQWQRGHLSNYQYLLHL NNLADRSCNDLSQYPVFPWIIHDYSSSELDLSNPGTFRDLSKPVGALNKERLERLLTRYQEMPEPKFMYG SHYSSPGYVLFYLVRIAPEYMLCLQNGRFDNADRMFNSIAETWKNCLDGATDFKELIPEFYGDDVSFLVN SLKLDLGKRQGGQMVDDVELPPWASSPEDFLQKSKDALESNYVSEHLHEWIDLIFGYKQKGSDAVGAHNV FHPLTYEGGVDLNSIQDPDEKVAMLTQILEFGQTPKQLFVTPHPRRITPKFKSLSQTSSYNASMADSPGE ESFEDLTEESKTLAWNNITKLQLHEHYKIHKEAVTGITVSRNGSSVFTTSQDSTLKMFSKESKMLQRSIS FSNMALSSCLLLPGDATVITSSWDNNVYFYSIAFGRRQDTLMGHDDAVSKICWHDNRLYSASWDSTVKVW SGVPAEMPGTKRHHFDLLAELEHDVSVDTISLNAASTLLVSGTKEGTVNIWDLTTATLMHQIPCHSGIVC DTAFSPDSRHVLSTGTDGCLNVIDVQTGMLISSMTSDEPQRCFVWDGNSVLSGSQSGELLVWDLLGAKIS ERIQGHTGAVTCIWMNEQCSSIITGGEDRQIIFWKLQY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001144772 |
| ORF Size | 2844 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001144772.1, NP_001138244.1 |
| RefSeq ORF | 2847 bp |
| Locus ID | 8439 |
| UniProt ID | Q92636 |
| Protein Families | Druggable Genome |
| MW | 107.1 kDa |
| Gene Summary | This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227565L3 | Lenti-ORF clone of NSMAF (Myc-DDK-tagged)-Human neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), transcript variant 2 |
CNY 9,030.00 |
|
| RC227565L4 | Lenti-ORF clone of NSMAF (mGFP-tagged)-Human neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), transcript variant 2 |
CNY 9,030.00 |
|
| RG227565 | NSMAF (tGFP-tagged) - Human neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), transcript variant 2 |
CNY 7,890.00 |
|
| SC326295 | NSMAF (untagged)-Human neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), transcript variant 2 |
CNY 18,340.00 |
