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TCHP (NM_001143852) Human Tagged ORF Clone
CAT＃: RC227291
TCHP (Myc-DDK-tagged)-Human trichoplein, keratin filament binding (TCHP), transcript variant 2
ORF Plasmid: tGFP
"NM_001143852" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TpMs |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC227291 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTCCCGACGCTGCCGTCCTACTGGTGCAGCCAGCAGCGCCTGAATCAGCAGCTAGCACGACAGC GAGAGCAGGAGGCCCGGCTTCGGCAGCAGTGGGAGCAGAACAGCCGTTACTTCAGGATGTCTGACATCTG CAGCTCCAAACAGGCAGAATGGAGCTCTAAAACCTCCTACCAGCGGAGCATGCATGCCTATCAGCGGGAG AAGATGAAGGAGGAGAAGAGGAGGAGTCTGGAGGCCCGACGGGAAAAGCTCAGGCAGCTCATGCAGGAGG AGCAGGACCTGCTGGCCAGAGAACTGGAGGAGCTGAGGCTGAGCATGAACTTGCAGGAAAGAAGAATCCG GGAGCAGCACGGGAAGCTGAAATCAGCCAAAGAAGAGCAGAGGAAACTGATTGCTGAACAACTTTTGTAC GAACACTGGAAAAAGAACAACCCGAAACTTCGAGAGATGGAGCTGGACCTTCACCAGAAGCATGTCGTAA ACTCTTGGGAAATGCAGAAAGAAGAAAAAAAACAGCAAGAAGCCACCGCAGAGCAAGAGAACAAACGGTA TGAAAATGAATATGAAAGGGCCCGAAGGGAGGCGCTAGAAAGGATGAAAGCTGAAGAGGAGAGGAGGCAG CTGGAGGACAAGCTCCAGGCCGAGGCACTGCTGCAACAGATGGAGGAGCTGAAGCTGAAGGAGGTGGAGG CGACCAAACTAAAGAAGGAGCAGGAGAATCTGTTGAAGCAGCGGTGGGAGCTAGAGAGGCTGGAGGAAGA GCGAAAGCAGATGGAAGCCTTCCGGCAGAAGGCAGAGCTGGGGCGTTTCTTGAGACATCAGTATAACGCT CAACTCAGCAGACGCACACAGCAGATCCAAGAGGAGCTGGAGGCAGACAGGCGGATCCTGCAGGCCCTCC TCGAGAAGGAGGACGAGAGCCAGCGCCTCCACCTGGCCAGGCGGGAGCAGGTCATGGCCGATGTGGCCTG GATGAAGCAGGCCATTGAGGAGCAGCTGCAGCTGGAGCGGGCGCGGGAGGCAGAGCTGCAGATGCTGCTG AGGGAGGAGGCCAAGGAGATGTGGGAAAAGAGAGAGGCAGAGTGGGCCCGAGAGCGCAGCGCACGGGACA GACTGATGAGCGAGGTTCTGACAGGGAGACAACAGCAAATACAAGAGAAGATTGAGCAGAACCGACGGGC ACAAGAGGAATCCCTGAAACACAGGGAGCAACTTATTCGAAATCTTGAGGAGGTGAGAGAGTTGGCTCGT CGCGAGAAAGAGGAGAGTGAAAAGCTGAAATCGGCCAGGAAGCAGGAGCTGGAAGCCCAGGTTGCAGAGC GCCGGCTGCAGGCATGGGAAGCAGACCAGCAGGAGGAGGAGGAAGAGGAGGAGGCCCGGCGGGTCGAGCA GCTCTCAGATGCCCTGCTGCAGCAGGAGGCGGAGACTATGGCTGAGCAGGGCTACCGGCCTAAGCCTTAC GGACATCCAAAAATTGCTTGGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227291 protein sequence
Red=Cloning site Green=Tags(s) MALPTLPSYWCSQQRLNQQLARQREQEARLRQQWEQNSRYFRMSDICSSKQAEWSSKTSYQRSMHAYQRE KMKEEKRRSLEARREKLRQLMQEEQDLLARELEELRLSMNLQERRIREQHGKLKSAKEEQRKLIAEQLLY EHWKKNNPKLREMELDLHQKHVVNSWEMQKEEKKQQEATAEQENKRYENEYERARREALERMKAEEERRQ LEDKLQAEALLQQMEELKLKEVEATKLKKEQENLLKQRWELERLEEERKQMEAFRQKAELGRFLRHQYNA QLSRRTQQIQEELEADRRILQALLEKEDESQRLHLARREQVMADVAWMKQAIEEQLQLERAREAELQMLL REEAKEMWEKREAEWARERSARDRLMSEVLTGRQQQIQEKIEQNRRAQEESLKHREQLIRNLEEVRELAR REKEESEKLKSARKQELEAQVAERRLQAWEADQQEEEEEEEARRVEQLSDALLQQEAETMAEQGYRPKPY GHPKIAWN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001143852 |
| ORF Size | 1494 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001143852.2 |
| RefSeq Size | 3111 bp |
| RefSeq ORF | 1497 bp |
| Locus ID | 84260 |
| UniProt ID | Q9BT92 |
| MW | 61.1 kDa |
| Gene Summary | Tumor suppressor which has the ability to inhibit cell growth and be pro-apoptotic during cell stress. Inhibits cell growth in bladder and prostate cancer cells by a down-regulation of HSPB1 by inhibiting its phosphorylation. May act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. May regulate K8/K18 filament and desmosome organization mainly at the apical or peripheral regions of simple epithelial cells (PubMed:15731013, PubMed:18931701). Is a negative regulator of ciliogenesis (PubMed:25270598).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227291L3 | Lenti ORF clone of Human trichoplein, keratin filament binding (TCHP), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC227291L4 | Lenti ORF clone of Human trichoplein, keratin filament binding (TCHP), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG227291 | TCHP (tGFP-tagged) - Human trichoplein, keratin filament binding (TCHP), transcript variant 2 |
CNY 4,370.00 |
|
| SC325135 | TCHP (untagged)-Human trichoplein, keratin filament binding (TCHP), transcript variant 2 |
CNY 6,270.00 |
