Phospholipase c eta 1 (PLCH1) (NM_001130960) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PLC-L3; PLC eta 1; PLCL3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC226422 representing NM_001130960 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACCTTGAAGTGTATAAAAACTTAAGTCCAGAAAAAGTTGAAAGATGCATGAGTGTGATGCAGT CCGGGACACAGATGATCAAGCTGAAACGTGGAACCAAAGGGCTTGTCCGCCTCTTTTACCTGGATGAGCA CCGGACACGCCTCCGATGGCGACCCTCTAGGAAGAGTGAGAAGGCAAAAATACTTATTGATTCCATTTAC AAAGTGACTGAGGGCCGGCAGTCTGAAATATTCCACAGACAAGCTGAGGGGAACTTCGACCCCAGCTGCT GCTTCACCATCTACCATGGCAACCACATGGAGTCCCTGGACCTCATCACCTCCAACCCCGAGGAGGCCCG CACCTGGATCACAGGCCTCAAGTACCTGATGGCTGGCATCAGTGATGAAGACTCCCTTGCCAAAAGGCAG AGGACCCATGACCAATGGGTGAAGCAGACCTTTGAGGAAGCTGATAAGAATGGTGACGGCTTGCTGAATA TTGAAGAGATACATCAGCTGATGCATAAACTGAATGTTAATCTGCCCCGAAGAAAAGTCAGACAAATGTT TCAGGAAGCCGACACAGATGAGAATCAGGGAACTTTGACATTTGAAGAGTTCTGTGTTTTTTACAAAATG ATGTCTTTGAGACGAGACCTTTATTTGTTACTTTTGAGCTACAGTGACAAGAAAGATCACCTAACTGTGG AAGAACTGGCTCAGTTTTTGAAGGTGGAGCAAAAGATGAATAATGTGACAACGGACTATTGTCTTGACAT CATAAAGAAGTTTGAAGTTTCAGAAGAAAATAAGGTGAAAAATGTTCTTGGCATAGAAGGCTTCACGAAC TTCATGCGTAGTCCTGCCTGTGACATATTTAACCCATTGCACCATGAAGTGTACCAAGACATGGATCAGC CCCTCTGCAACTACTACATTGCTTCCTCTCACAATACATACCTGACTGGAGACCAGCTCCTTTCTCAGTC CAAAGTGGATATGTATGCACGGGTGCTGCAAGAGGGCTGTCGCTGTGTGGAAGTTGACTGTTGGGATGGC CCAGATGGAGAGCCAGTAGTACATCATGGTTACACTCTCACTTCAAAAATTCTCTTCAGAGATGTTGTGG AGACCATCAACAAGCATGCCTTTGTGAAGAATGAGTTTCCTGTTATATTGTCTATCGAGAATCACTGCAG TATCCAGCAGCAAAGGAAGATTGCTCAGTACCTGAAAGGAATATTCGGAGACAAACTGGACCTGTCATCT GTTGATACAGGGGAGTGCAAGCAGCTTCCAAGCCCTCAAAGTTTGAAAGGCAAAATTCTAGTGAAGGGTA AGAAGTTGCCTTATCACCTTGGGGATGATGCAGAGGAAGGGGAAGTTTCTGATGAGGACAGTGCAGATGA AATTGAAGACGAGTGCAAATTCAAGCTCCATTATAGTAATGGGACCACTGAGCATCAGGTGGAATCTTTC ATAAGGAAAAAACTGGAGTCACTGTTAAAAGAATCTCAAATTCGAGATAAAGAAGATCCTGATAGTTTCA CAGTGCGGGCACTACTGAAGGCCACGCATGAAGGCTTAAATGCACACCTGAAGCAGAGTCCAGATGTAAA GGAAAGTGGAAAGAAATCACATGGACGATCCCTCATGACCAACTTTGGAAAACATAAGAAAACTACAAAA TCACGGTCTAAATCTTACAGTACTGATGATGAGGAAGACACACAGCAGAGTACTGGCAAGGAGGGTGGCC AGCTGTACAGATTGGGTCGCCGAAGGAAAACCATGAAGCTCTGCCGAGAACTCTCTGATTTGGTTGTGTA CACAAACTCCGTGGCCGCTCAGGACATTGTGGATGACGGAACCACAGGAAATGTGTTATCATTCAGTGAA ACAAGAGCACATCAGGTTGTTCAGCAAAAATCAGAGCAGTTCATGATTTATAATCAAAAGCAACTCACGA GGATTTACCCCTCTGCCTACCGCATTGATTCCAGTAACTTCAACCCTCTCCCCTACTGGAACGCAGGCTG CCAGCTAGTGGCACTGAATTATCAATCTGAAGGACGAATGATGCAGTTAAACCGAGCCAAATTCAAGGCA AATGGCAATTGTGGCTATGTCCTCAAACCCCAGCAAATGTGCAAAGGTACTTTCAACCCTTTCTCTGGTG ACCCTCTTCCTGCCAACCCCAAAAAGCAGCTCATCCTGAAAGTTATCAGTGGACAGCAACTCCCCAAACC TCCAGACTCCATGTTTGGAGATCGAGGCGAGATCATTGACCCTTTTGTTGAAGTTGAAATTATTGGATTG CCAGTAGATTGTTGTAAAGATCAAACCCGTGTGGTAGATGACAATGGATTTAACCCTGTGTGGGAAGAAA CACTGACATTTACAGTACACATGCCAGAAATAGCTTTGGTTCGGTTCCTTGTGTGGGATCACGATCCCAT TGGACGAGACTTTGTTGGACAAAGAACTGTGACCTTCAGCAGCTTAGTGCCTGGCTACCGGCATGTCTAT TTGGAAGGACTGACAGAAGCATCCATATTTGTACACATAACCATCAATGAAATCTATGGAAAGTGGAGCC CTTTAATACTCAACCCCAGTTATACTATATTGCACTTTCTAGGAGCTACAAAGAACAGACAACTCCAGGG TCTGAAGGGACTGTTCAATAAGAATCCTAGGCACAGTTCTTCAGAAAACAATTCCCATTATGTACGGAAG CGATCCATTGGAGATAGAATTCTGCGACGCACAGCTAGCGCCCCAGCCAAAGGCAGGAAAAAGAGCAAAA TGGGCTTCCAAGAAATGGTGGAGATAAAGGATTCTGTGTCCGAGGCCACAAGAGATCAAGATGGCGTGCT GAGGAGGACCACACGCAGTTTGCAAGCACGCCCTGTCTCTATGCCTGTTGACAGAAACCTTCTGGGAGCT TTGTCGCTGCCTGTATCTGAAACAGCAAAAGACATTGAAGGAAAAGAAAACTCTCTAGCAGAAGATAAAG ATGGCAGAAGAAAAGGGAAAGCAAGTATAAAAGATCCACATTTTCTAAATTTCAACAAAAAGTTATCATC CTCCTCCAGTGCTCTGCTCCACAAAGATACCAGCCAAGGGGACACCATTGTATCTACTGCCCACATGTCA GTCACAGGAGAACAGCTGGGCATGTCAAGTCCTAGGGGTGGGAGAACCACATCAAATGCCACAAGCAATT GCCAGGAAAACCCCTGTCCCAGCAAGTCTCTCTCCCCAAAGCAGCATTTGGCTCCCGATCCTGTAGTTAA CCCCACACAAGATCTGCATGGTGTGAAAATCAAGGAAAAGGGTAATCCTGAGGACTTTGTGGAAGGGAAA AGCATCTTGTCAGGAAGCGTCCTTTCTCATAGCAACCTAGAAATTAAGAACCTGGAAGGTAATAGGGGTA AGGGCCGAGCTGCAACATCCTTTTCTTTGTCAGACGTCTCCATGCTCTGTTCTGACATACCTGACCTACA TTCAACTGCAATTCTGCAGGAGAGTGTAATTTCCCATCTTATTGACAATGTCACTTTAACAAATGAGAAT GAGCCGGGCAGTTCCATCTCAGCCCTGATTGGCCAGTTTGATGAGACCAACAATCAGGCTCTCACAGTTG TTTCTCATCTTCATAATACCAGTGTGATGTCAGGCCATTGTCCCTTGCCTAGCCTGGGCCTAAAAATGCC CATCAAGCATGGTTTTTGCAAGGGAAAATCCAAGTCTTCCTTCCTGTGCTCATCTCCGGAGCTGATAGCA CTCTCGAGTTCTGAGACCACCAAACATGCAACGAACACAGTTTATGAAACTACCTGCACTCCCATCTCTA AAACCAAACCAGATGATGACCTTTCTAGTAAGGCCAAGACAGCGGCCTTAGAAAGCAACCTGCCTGGATC CCCTAATACTTCTCGTGGCTGGTTACCAAAAAGTCCTACCAAGGGAGAAGACTGGGAAACACTGAAGAGC TGCAGCCCTGCCTCTTCCCCTGATTTGACCCTGGAGGATGTAATAGCTGATCCCACTCTCTGTTTCAATT CTGGGGAGAGCAGCCTTGTGGAAATTGATGGAGAATCAGAAAATCTTTCTCTAACAACCTGTGAATATAG GAGAGAGGGCACAAGTCAACTTGCTTCTCCTTTAAAACTCAAGTACAATCAGGGTGTGGTAGAACACTTT CAAAGAGGTTTGAGAAACGGCTACTGTAAAGAGACCCTCCGCCCTTCTGTCCCTGAAATATTCAACAATA TTCAAGATGTCAAAACTCAAAGTATTTCTTATCTAGCCTATCAGGGTGCTGGCTTTGTGCATAATCATTT CTCAGATTCAGATGCAAAAATGTTCCAGACCTGTGTGCCCCAGCAATCTAGTGCTCAAGATATGCATGTC CCTGTACCCAAGCAGTTGGCACATCTTCCTTTGCCTGCTCTGAAACTGCCTAGTCCTTGCAAATCCAAAA GTCTGGGGGACTTAACATCAGAGGACATTGCCTGCAATTTTGAGAGCAAGTACCAGTGTATTAGTAAGAG TTTTGTTACAACTGGCATTAGAGACAAGAAGGGCGTGACTGTGAAGACAAAGTCGTTAGAGCCTATAGAT GCCCTGACCGAGCAGCTTCGGAAGCTTGTGTCCTTTGACCAGGAAGACAACTGCCAAGTGCTATATTCAA AGCAGGATGCCAATCAGCTCCCCCGGGCACTGGTCAGGAAGTTGTCATCCAGAAGTCAGAGCAGAGTGCG CAATATTGCTAGTCGTGCCAAGGAGAAACAGGAAGCCAACAAGCAGAAAGTTCCAAACCCCAGCAATGGG GCAGGAGTGGTTCTTAGAAACAAACCCTCAGCACCCACCCCTGCAGTGAATCGCCACTCCACCGGCTCCT ACATCGCAGGCTACCTGAAGAACACGAAAGGGGGTGGCCTTGAAGGCCGGGGCATCCCAGAGGGGGCATG CACGGCTCTTCACTATGGCCACGTTGACCAGTTTTGTTCAGATAATTCTGTTTTGCAGACTGAGCCAAGC AGTGATGATAAACCAGAAATTTATTTTCTTTTGAGACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC226422 representing NM_001130960 Red=Cloning site Green=Tags(s) MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIY KVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQ RTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKM MSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTN FMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDG PDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSS VDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESF IRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTK SRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSE TRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKA NGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGL PVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVY LEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRK RSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGA LSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMS VTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGK SILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNEN EPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIA LSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKS CSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHF QRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHV PVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPID ALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNG AGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPS SDDKPEIYFLLRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001130960
ORF Size	5079 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001130960.2
RefSeq ORF	5082 bp
Locus ID	23007
UniProt ID	Q4KWH8
Protein Families	Druggable Genome
MW	189 kDa
Gene Summary	PLCH1 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to generate second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) (Hwang et al., 2005 [PubMed 15702972]).[supplied by OMIM, Jun 2009]

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